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Q9V407 (AXN_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Axin
Alternative name(s):
Axis inhibition protein
d-Axin
Short name=dAxin
Gene names
Name:Axn
ORF Names:CG7926
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length745 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitor of the WG signaling pathway. Down-regulates beta-catenin (armadillo=ARM). Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B (zeste-white 3=ZW3). Ref.5

Subunit structure

Interacts with ZW3 and ARM. The interaction between AXN and ARM occurs via the armadillo repeats contained in ARM.

Subcellular location

Cytoplasm By similarity.

Developmental stage

Ubiquitously expressed throughout the development.

Sequence similarities

Contains 1 DIX domain.

Contains 1 RGS domain.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentCytoplasm
   Molecular functionDevelopmental protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from mutant phenotype PubMed 18561909. Source: FlyBase

eye-antennal disc morphogenesis

Inferred from mutant phenotype PubMed 11290291. Source: FlyBase

heart development

Inferred from mutant phenotype PubMed 22398840. Source: FlyBase

imaginal disc pattern formation

Inferred from mutant phenotype Ref.5. Source: FlyBase

imaginal disc-derived wing morphogenesis

Inferred from mutant phenotype Ref.5. Source: FlyBase

negative regulation of Wnt signaling pathway

Inferred from direct assay PubMed 16314428. Source: FlyBase

oogenesis

Inferred from mutant phenotype PubMed 12783796. Source: FlyBase

phagocytosis

Inferred from mutant phenotype PubMed 16336044. Source: FlyBase

positive regulation of GTPase activity

Inferred from Biological aspect of Ancestor. Source: GOC

somatic stem cell maintenance

Inferred from mutant phenotype PubMed 12783796. Source: FlyBase

termination of G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 14981260PubMed 21245303. Source: FlyBase

nucleus

Inferred from direct assay PubMed 14981260. Source: FlyBase

plasma membrane

Inferred from direct assay PubMed 21245303. Source: FlyBase

   Molecular_functionGTPase activator activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

beta-catenin binding

Inferred from physical interaction PubMed 19850033. Source: FlyBase

protein kinase binding

Inferred from physical interaction PubMed 19850033. Source: FlyBase

signal transducer activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 745745Axin
PRO_0000220894

Regions

Domain54 – 172119RGS
Domain663 – 74583DIX
Compositional bias640 – 6467Poly-Ser

Amino acid modifications

Modified residue5131Phosphothreonine Ref.6
Modified residue5171Phosphoserine Ref.6
Modified residue5221Phosphoserine Ref.6

Experimental info

Sequence conflict4541R → Q in AAD24886. Ref.1
Sequence conflict644 – 6452Missing in AAD24886. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9V407 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 31A502528CEE84BA

FASTA74581,718
        10         20         30         40         50         60 
MSGHPSGIRK HDDNECSGPR PPVPGEESRV KKMTEGVADT SKNSSPSYLN WARTLNHLLE 

        70         80         90        100        110        120 
DRDGVELFKK YVEEEAPAYN DHLNFYFACE GLKQQTDPEK IKQIIGAIYR FLRKSQLSIS 

       130        140        150        160        170        180 
DDLRAQIKAI KTNPEIPLSP HIFDPMQRHV EVTIRDNIYP TFLCSEMYIL YIQQMSAQQE 

       190        200        210        220        230        240 
RCTSSGATGS GSAGSSGSGG SSLAGACALP PTTASGKQQL PQLVPPGAFI NLPVSSVSGP 

       250        260        270        280        290        300 
PAGTCSASGS VYGPSTSASS SGSISATDTL PRSSTLPTLH EDSVLSLCDD FEKVQMQEGG 

       310        320        330        340        350        360 
GSLGSGSVGA GARAPDYPIR LTRDLLIATQ KRRLEIRPPG AHGYVYNPST TNTSYVPNSR 

       370        380        390        400        410        420 
VDSERASVSS GGRTDSDTMS ISSCSMDGRP YIQRRHSSTE SKAIRQSAMA NKETNTFQVI 

       430        440        450        460        470        480 
PRTQRLHSNE HRPLKEEELV SLLIPKLEEV KRKRDLEERA RERNPGAALL TNERSSASDR 

       490        500        510        520        530        540 
AFAEAIREKF ALDEDNDQDI LDQHVSRVWK DQTPHRSPGT MSPCPPIPSR RRTATHDSGM 

       550        560        570        580        590        600 
VSDGAMSLSG HSMKHSKSMP DHSSCSRKLT NKWPSMNTDS GISMFSADTV TKYKDASSRS 

       610        620        630        640        650        660 
GSSTASKLEE AKRRLEDEPR RSRRYAQPPM QHLSQQPLAS FSSSSSGGSI SLPHQPPPLP 

       670        680        690        700        710        720 
AKPPETIVVF SFCEEPVPYR IKIPGTQPTL RQFKDYLPRR GHFRFFFKTH CEDPDSPVIQ 

       730        740 
EEIVNDSDIL PLFGDKAMGL VKPSD 

« Hide

References

« Hide 'large scale' references
[1]"Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin."
Hamada F., Tomoyasu Y., Takatsu Y., Nakamura M., Nagai S., Suzuki A., Fujita F., Shibuya H., Toyoshima K., Ueno N., Akiyama T.
Science 283:1739-1742(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[2]"A Drosophila homolog of the axin gene is involved in the transduction of the wingless signal regulating the stability of the armadillo protein."
Ruel L., Anthopoulos N., Goncalves J., Manoukian A.S., Woodgett J.R.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[5]"A Drosophila Axin homolog, Daxin, inhibits Wnt signaling."
Willert K., Logan C.Y., Arora A., Fish M., Nusse R.
Development 126:4165-4173(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-513; SER-517 AND SER-522, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF086811 mRNA. Translation: AAD24886.1.
AF091813 mRNA. Translation: AAF21293.1.
AE014297 Genomic DNA. Translation: AAF56993.1.
RefSeqNP_733336.1. NM_170457.3.
NP_733337.1. NM_170458.2.
UniGeneDm.7154.

3D structure databases

ProteinModelPortalQ9V407.
SMRQ9V407. Positions 46-171, 663-745.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid68421. 14 interactions.
DIPDIP-20929N.
IntActQ9V407. 1 interaction.
MINTMINT-926493.

Proteomic databases

PaxDbQ9V407.
PRIDEQ9V407.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0085553; FBpp0084919; FBgn0026597.
FBtr0085555; FBpp0084921; FBgn0026597.
GeneID43565.
KEGGdme:Dmel_CG7926.
UCSCCG7926-RA. d. melanogaster.

Organism-specific databases

CTD43565.
FlyBaseFBgn0026597. Axn.

Phylogenomic databases

eggNOGNOG238205.
GeneTreeENSGT00390000010011.
InParanoidQ9V407.
KOK02157.
OMARQSAMAN.
OrthoDBEOG79PJQ0.
PhylomeDBQ9V407.

Enzyme and pathway databases

SignaLinkQ9V407.

Gene expression databases

BgeeQ9V407.

Family and domain databases

Gene3D1.10.196.10. 2 hits.
InterProIPR014936. Axin_b-cat-bd.
IPR001158. DIX.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamPF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
SMARTSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEPS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi43565.
NextBio834579.
PROQ9V407.

Entry information

Entry nameAXN_DROME
AccessionPrimary (citable) accession number: Q9V407
Secondary accession number(s): Q9XYC1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase