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Protein

Serine protease 7

Gene

Sp7

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease which, by cleaving and activating prophenoloxidase (PPO1) after immune challenge, plays an essential role in the melanization immune response to septic wounding (PubMed:16256951, PubMed:16322759, PubMed:19071960, PubMed:24260243, PubMed:16861233). May function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins; Spn27A in the hemolymph and Spn77BA in the trachea (PubMed:18854145, PubMed:24260243, PubMed:16322759). Important for the innate immune response to fungi (PubMed:16861233). Regulation of melanization and PPO1 activation appears to be largely independent of the Toll signaling pathway (PubMed:16861233).6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei182Charge relay systemPROSITE-ProRule annotation1
Active sitei244Charge relay systemPROSITE-ProRule annotation1
Active sitei341Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • melanization defense response Source: FlyBase
  • positive regulation of melanization defense response Source: FlyBase
  • proteolysis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.203.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease 71 Publication (EC:3.4.21.-1 Publication)
Alternative name(s):
Melanization protease 21 Publication
Gene namesi
Name:Sp7Imported
Synonyms:MP21 Publication, PAE11 Publication
ORF Names:CG3066Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0037515. Sp7.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown is semi-pupal lethal, with 50% of pupae dying at the late pupal stage or during eclosion (PubMed:16861233). Adults display impaired melanization at the site of septic infection (septic injury) using either Gram-negative or Gram-positive bacteria (PubMed:16256951, PubMed:16861233, PubMed:19071960). Reduced survival, PPO1 activity and induction of the antimicrobial peptide Drs following septic injury using the fungus B.bassiana (PubMed:16256951, PubMed:16861233). Septic injury with various bacteria reduces survival (L.monocytogenes, S.typhimurium and S.aureus) and in some cases decreases (E.faecelis) or increases bacterial growth (L.monocytogenes, S.typhimurium and B.cepacia). Aseptic injury reduces survival (PubMed:19071960). Aseptic wounding has no effect on survival (PubMed:22227521). Significant increase in survival after immune challenge with S.pneumoniae although there is no increase in melanization (PubMed:19071960). No effect on the survival following infection with various bacteria (E.coli, B.cepacia and E.faecelis) (PubMed:19071960). No induction of the antimicrobial peptides Drs and Dpt following infection with E.carotovora (PubMed:16861233).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000043811328 – 136Activation peptide1 PublicationAdd BLAST109
ChainiPRO_5007718041137 – 391Serine protease 71 PublicationAdd BLAST255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi141N-linked (GlcNAc...)PROSITE-ProRule annotation1
Disulfide bondi167 ↔ 183PROSITE-ProRule annotation
Disulfide bondi310 ↔ 327PROSITE-ProRule annotation
Disulfide bondi337 ↔ 366PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei136 – 137Cleavage1 Publication2

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiF7VJU2.
Q6BD09.
PRIDEiQ6BD09.

Expressioni

Developmental stagei

First detected in stage 11 embryos, in cells close to the gnathal region. At stage 13, expressed in numerous cells that are dispersed throughout the embryo. During organogenesis predominantly expressed under the cuticle in the proventricular zone close to the hindgut. In L3 larvae, expressed in the lymph glands, the hematopoietic organ that flanks the dorsal vessel and in mature hemolymph crystal cells that appear in clusters attached to diverse organs.1 Publication

Inductioni

Up-regulated after wounding. Levels are higher with septic wounding, using either Gram-negative or Gram-positive bacteria.1 Publication

Gene expression databases

BgeeiFBgn0037515.

Interactioni

Subunit structurei

Interacts with Spn27A.1 Publication

Protein-protein interaction databases

MINTiMINT-336596.
STRINGi7227.FBpp0081237.

Structurei

3D structure databases

ProteinModelPortaliQ9V3Z2.
SMRiQ9V3Z2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini137 – 390Peptidase S1PROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00860000133744.
OMAiFATRNIH.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ9V3Z2.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR022700. CLIP.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF12032. CLIP. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00680. CLIP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9V3Z2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSTRKVVGI FLATCLLPFT VLQNVAAQGS CRNPNQKQGQ CLSIYDCQSL
60 70 80 90 100
LSVIQQSYVS PEDRTFLRNS QCLDGVGRQP YVCCTSDRSF GSQEATSAAP
110 120 130 140 150
PPTTTSSSSR GQDGQAGLGN LLPSPPKCGP HSFSNKVYNG NDTAIDEFNW
160 170 180 190 200
MALLEYVDNR GRRELSCGGS LINNRYVLTA AHCVIGAVET EVGHLTTVRL
210 220 230 240 250
GEYDTSKDVD CIDDICNQPI LQLGIEQATV HPQYDPANKN RIHDIALLRL
260 270 280 290 300
DRPVVLNEYI QPVCLPLVST RMAINTGELL VVSGWGRTTT ARKSTIKQRL
310 320 330 340 350
DLPVNDHDYC ARKFATRNIH LISSQLCVGG EFYRDSCDGD SGGPLMRRGF
360 370 380 390
DQAWYQEGVV SFGNRCGLEG WPGVYTRVAD YMDWIVETIR P
Length:391
Mass (Da):43,128
Last modified:May 1, 2000 - v1
Checksum:i3E1416F12EDF89F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF54143.1.
AE014297 Genomic DNA. Translation: AAG22126.1.
AE014297 Genomic DNA. Translation: AAG22127.2.
AF233093 mRNA. Translation: AAF43410.1.
AY665379 mRNA. Translation: AAT76546.1.
BT128742 mRNA. Translation: AEH59645.1.
RefSeqiNP_649734.2. NM_141477.2.
NP_731174.1. NM_169192.3.
NP_731175.2. NM_169193.3.
UniGeneiDm.6983.

Genome annotation databases

EnsemblMetazoaiFBtr0081740; FBpp0081237; FBgn0037515.
FBtr0339180; FBpp0308321; FBgn0037515.
FBtr0339181; FBpp0308322; FBgn0037515.
GeneIDi40918.
KEGGidme:Dmel_CG3066.
UCSCiCG3066-RA. d. melanogaster.
CG3066-RB. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF54143.1.
AE014297 Genomic DNA. Translation: AAG22126.1.
AE014297 Genomic DNA. Translation: AAG22127.2.
AF233093 mRNA. Translation: AAF43410.1.
AY665379 mRNA. Translation: AAT76546.1.
BT128742 mRNA. Translation: AEH59645.1.
RefSeqiNP_649734.2. NM_141477.2.
NP_731174.1. NM_169192.3.
NP_731175.2. NM_169193.3.
UniGeneiDm.6983.

3D structure databases

ProteinModelPortaliQ9V3Z2.
SMRiQ9V3Z2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-336596.
STRINGi7227.FBpp0081237.

Protein family/group databases

MEROPSiS01.203.

Proteomic databases

PaxDbiF7VJU2.
Q6BD09.
PRIDEiQ6BD09.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081740; FBpp0081237; FBgn0037515.
FBtr0339180; FBpp0308321; FBgn0037515.
FBtr0339181; FBpp0308322; FBgn0037515.
GeneIDi40918.
KEGGidme:Dmel_CG3066.
UCSCiCG3066-RA. d. melanogaster.
CG3066-RB. d. melanogaster.

Organism-specific databases

CTDi121340.
FlyBaseiFBgn0037515. Sp7.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00860000133744.
OMAiFATRNIH.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ9V3Z2.

Miscellaneous databases

GenomeRNAii40918.

Gene expression databases

BgeeiFBgn0037515.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR022700. CLIP.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF12032. CLIP. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00680. CLIP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSER7_DROME
AccessioniPrimary (citable) accession number: Q9V3Z2
Secondary accession number(s): F7VJU2, Q6BD09, Q9I7L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2016
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.