Q9V3U0 (BGML_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Long-chain-fatty-acid--CoA ligase bubblegum-like
EC=6.2.1.3

Gene names

ORF Names:

CG4500

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	681 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mediates activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.
Sequence caution	The sequence AAM12254.1 differs from that shown. Reason: Erroneous termination at position 195. Translated as Gln.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	long-chain fatty acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB mesoderm development Inferred from expression pattern. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain fatty acid-CoA ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 681	681	Long-chain-fatty-acid--CoA ligase bubblegum-like		PRO_0000315818
Regions
	Nucleotide binding	223 – 231	9	ATP By similarity
	Nucleotide binding	414 – 419	6	ATP By similarity
Sites
	Binding site	491	1	ATP By similarity
	Binding site	506	1	ATP By similarity
	Binding site	639	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V3U0 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 6310902CC494712E	FASTA	681	75,152
	10 20 30 40 50 60 MNDLKPATSY RSTSLHDAVK LRLDEPSSFS QTVPPQTIPE FFKESCEKYS DLPALVWETP 70 80 90 100 110 120 GSGNDGWTTL TFGEYQERVE QAALMLLSVG VEERSSVGIL AFNCPEWFFA EFGALRAGAV 130 140 150 160 170 180 VAGVYPSNSA EAVHHVLATG ESSVCVVDDA QQMAKLRAIK ERLPRLKAVI QLHGPFEAFV 190 200 210 220 230 240 DHEPGYFSWQ KLQEQTFSSE LKEELLARES RIRANECAML IFTSGTVGMP KAVMLSHDNL 250 260 270 280 290 300 VFDTKSAAAH MQDIQVGKES FVSYLPLSHV AAQIFDVFLG LSHAGCVTFA DKDALKGTLI 310 320 330 340 350 360 KTFRKARPTK MFGVPRVFEK LQERLVAAEA KARPYSRLLL ARARAAVAEH QTTLMAGKSP 370 380 390 400 410 420 SIYGNAKYWL ACRVVKPIRE MIGVDNCRVF FTGGAPTSEE LKQFFLGLDI ALGECYGMSE 430 440 450 460 470 480 TSGAITLNVD ISNLYSAGQA CEGVTLKIHE PDCNGQGEIL MRGRLVFMGY LGLPDKTEET 490 500 510 520 530 540 VKEDGWLHSG DLGYIDPKGN LIISGRLKEL IITAGGENIP PVHIEELIKK ELPCVSNVLL 550 560 570 580 590 600 IGDHRKYLTV LLSLKTKCDA KTGIPLDALR EETIEWLRDL DIHETRLSEL LNIPADLQLP 610 620 630 640 650 660 NDTAALAATL EITAKPKLLE AIEEGIKRAN KYAISNAQKV QKFALIAHEF SVATGELGPT 670 680 LKIRRNIVHA KYAKVIERLY K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-681. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014134 Genomic DNA. Translation: AAF53370.1. BT021247 mRNA. Translation: AAX33395.1. AY095523 mRNA. Translation: AAM12254.1. Sequence problems.
RefSeq	NP_609696.1. NM_135852.3.
3D structure databases
ProteinModelPortal	Q9V3U0.
SMR	Q9V3U0. Positions 505-556.
ModBase	Search...
Proteomic databases
PRIDE	Q9V3U0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0080591; FBpp0080168; FBgn0028519.
GeneID	34822.
KEGG	dme:Dmel_CG4500.
NMPDR	fig|7227.3.peg.2375.
UCSC	CG4500-RA. d. melanogaster.
Organism-specific databases
FlyBase	FBgn0028519. CG4500.
Phylogenomic databases
eggNOG	inNOG08537.
GeneTree	EMGT00050000004540.
InParanoid	Q9V3U0.
OMA	CAMLIFT.
OrthoDB	EOG45HQCB.
PhylomeDB	Q9V3U0.
Gene expression databases
ArrayExpress	Q9V3U0.
Bgee	Q9V3U0.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	790390.

Entry information

Entry name

BGML_DROME

Accession

Primary (citable) accession number: Q9V3U0
Secondary accession number(s): Q8SWR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project