Q9V3R1 (A36DE_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Accessory gland protein Acp36DE

Gene names

Name:	Acp36DE
ORF Names:	CG7157

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	912 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for physiological and behavioral changes in mated female flies. Associates with sperm and localizes to specific regions of the female reproductive tract, including the sperm storage organs. It accelerates sperm accumulation into storage but does not mediate the entry of the first sperm into storage. Once sperm storage has initiated it seems to acts as a guidance factor helping subsequent sperm move into storage, a corral concentrating sperm around the SSO entrances and/or a trigger for responses within the female that accelerate storage of sperm. Ref.1 Ref.8
Subcellular location	Secreted Probable.
Tissue specificity	Main cells of accessory gland and seminal fluid. Ref.1

Ontologies

Keywords
Biological process	Behavior
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mating plug formation Traceable author statement. Source: FlyBase negative regulation of female receptivity, post-mating Non-traceable author statement Ref.1. Source: UniProtKB sperm storage Inferred from mutant phenotype. Source: FlyBase
Cellular component	extracellular space Inferred from direct assay. Source: FlyBase
Molecular function	hormone activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 912	889	Accessory gland protein Acp36DE		PRO_0000020583
Regions
	Compositional bias	183 – 897	715	Gln/Ser-rich
Natural variations
	Natural variant	148	1	N → S in strain: WSII16.
	Natural variant	190	1	A → V in strain: WSII16.
	Natural variant	198	1	T → S in strain: SENGWA2, SENGWA22, SENGWA29, SENGWA32, SENGWA37, SENGWA51, WSII19 and WSII49.
	Natural variant	223	1	Missing in strain: SENGWA22.
	Natural variant	231	1	E → G in strain: WSII6.
	Natural variant	253	1	Q → QSQ in strain: SENGWA2, SENGWA29, SENGWA32, SENGWA51, WSII19 and WSII49.
	Natural variant	307	1	E → G in strain: WSII6.
	Natural variant	316	1	I → F in strain: WSII16.
	Natural variant	325	1	K → T in strain: SENGWA37.
	Natural variant	335	1	S → P in strain: SENGWA30.
	Natural variant	352	1	Q → QLLREAQQK in strain: SENGWA2, SENGWA22, SENGWA29, SENGWA30, SENGWA32, SENGWA37, SENGWA51, WSII16, WSII19 and WSII49.
	Natural variant	385	1	F → HQN in strain: WSII1.
	Natural variant	430	1	V → I in strain: WSII16.
	Natural variant	464	1	L → F in strain: Canton-S, SENGWA2, SENGWA22, SENGWA29, SENGWA32 and SENGWA51.
	Natural variant	496	1	E → G in strain: WSII6.
	Natural variant	507	1	L → V in strain: SENGWA22, SENGWA29, SENGWA37 and SENGWA51.
	Natural variant	575	1	L → Q in strain: WSII16.
	Natural variant	601	1	S → I in strain: WSII26 and WSII49.
	Natural variant	613	1	E → K in strain: SENGWA30.
	Natural variant	650	1	P → A in strain: SENGWA22, SENGWA30 and WSII16.
	Natural variant	666	1	L → V in strain: SENGWA29, SENGWA30, SENGWA37 and SENGWA51.
	Natural variant	670	1	G → E in strain: SENGWA29, SENGWA30, SENGWA37 and SENGWA51.
	Natural variant	758	1	T → A in strain: SENGWA37 and SENGWA51.
	Natural variant	782 – 786	5	Missing in strain: SENGWA37 and SENGWA51.
	Natural variant	830	1	Q → E in strain: WSII26.
	Natural variant	844	1	Q → R in strain: WSII16.

Sequences

	Sequence		Length	Mass (Da)
	Q9V3R1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 81E2C53431EAFBC5	FASTA	912	101,921
	10 20 30 40 50 60 MWTLTCQQFI ALILLGTLVP SESFLCKHCF RKNLEKVHES FRDILSPPIF GVNPQPLIEV 70 80 90 100 110 120 QQPKVTPKPE SSQVIHVHQP QVILKPIYYP KVDTISTKNQ IGIHGPYSQY PSLLPSANLL 130 140 150 160 170 180 GIPNQQLINA QDVLSDKDQK QTQVQNNNLH IRFGVSALRE GRNNPSLETI SRDKVDKISP 190 200 210 220 230 240 ALQLQLLRYA DSQSQSQTQS QSASQSESNA SSQFQAQEQS NRLLENPPVS ESQSQSESQS 250 260 270 280 290 300 QSESQKQSQS QSQRQQQIQT QLQILRQLQQ KSNEQSAAQS ASQIQSQRQS DSQSNLQLQE 310 320 330 340 350 360 QSQSQSEQGK PIQSQIQILQ GLQQKELDDK SASQSQSESK TRQEQQKQLN LQQLEELSSS 370 380 390 400 410 420 LSQSRLGLGQ QIQSQLQKNQ LDKQFASQFQ SQSKSQLEQQ MQLQLQSLRQ LQQKQLDEQS 430 440 450 460 470 480 ASQSQPQSQV AQQIQSHLQL LRLLQSRLKT QSALKSDLEQ QILLQLKKLT EVQQKQLAEQ 490 500 510 520 530 540 PTLRPSSKSQ SPGQLEQQIL LQLQNLLQFQ QNQLKSDTQT QSQLQESKSN SLSQSQSQSQ 550 560 570 580 590 600 EQLQLQRDQN LRQLEQIKLE MQNIRELLQK GKSELQTQSD SQRRIHELYQ NILQLNKEKL 610 620 630 640 650 660 SYQLKQLKLK ELEDQKKSQA EISKGSNPSN LFIIGQLPSE GKPAPGNQGP SIEPKLVPQP 670 680 690 700 710 720 GSLDKLPSGG GLIGKPASTG LYILSPDFND LSDYRDQFRL QQELKKHQNI LSLLQRRQND 730 740 750 760 770 780 IKKQQNAQLL LGQQQKEQQA QESINKQQSS SAGSSSQTKL QQDIQSTGAQ GSQQGLQAGS 790 800 810 820 830 840 TGLQTSSLQG TESSASQSAA LQRLKEQEQL RIQTENDQKT SSSSSHSNSQ NSQSSSSQSS 850 860 870 880 890 900 QASQSEAQRQ EAGNRNTLLL DQSSSKTQSE SKSESSSQSS SHSSSQSTSN SSSNVQSKLQ 910 GESQALLNNL SG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New genes for male accessory gland proteins in Drosophila melanogaster." Wolfner M.F., Harada H.A., Bertram M.J., Stelick T.J., Kraus K.W., Kalb J.M., Lung Y.O., Neubaum D.M., Park M., Tram U.K. Insect Biochem. Mol. Biol. 27:825-834(1997) [PubMed: 9474779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, TISSUE SPECIFICITY. Strain: Canton-S. Tissue: Male accessory gland.
[2]	Wolfner M.F., Harada H.A., Bertram M.J., Stelick T.J., Kraus K.W., Kalb J.M., Lung Y.O., Neubaum D.M., Park M., Tram U.K. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO N-TERMINUS AND C-TERMINUS.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[6]	"Molecular population genetics of male accessory gland proteins in Drosophila." Begun D.J., Whitley P., Todd B.L., Waldrip-Dail H.M., Clark A.G. Genetics 156:1879-1888(2000) [PubMed: 11102381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 144-889. Strain: SENGWA2, SENGWA22, SENGWA29, SENGWA30, SENGWA32, SENGWA37, SENGWA51, WSII1, WSII12, WSII16, WSII19, WSII26, WSII47, WSII49, WSII6 and WSII9.
[7]	"A Drosophila complementary DNA resource." Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M., Harvey D.A. Science 287:2222-2224(2000) [PubMed: 10731138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 401-912. Strain: Berkeley. Tissue: Head.
[8]	"An early role for the Drosophila melanogaster male seminal protein Acp36DE in female sperm storage." Bloch-Qazi M.C., Wolfner M.F. J. Exp. Biol. 206:3521-3528(2003) [PubMed: 12939382] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U85759 mRNA. Translation: AAB96383.2. AF157488 Genomic DNA. Translation: AAD40185.1. AE014134 Genomic DNA. Translation: AAF53664.1. AY113344 mRNA. Translation: AAM29349.1. AY010577 Genomic DNA. Translation: AAG37359.1. AY010578 Genomic DNA. Translation: AAG37360.1. AY010579 Genomic DNA. Translation: AAG37361.1. AY010580 Genomic DNA. Translation: AAG37362.1. AY010581 Genomic DNA. Translation: AAG37363.1. AY010582 Genomic DNA. Translation: AAG37364.1. AY010583 Genomic DNA. Translation: AAG37365.1. AY010584 Genomic DNA. Translation: AAG37366.1. AY010585 Genomic DNA. Translation: AAG37367.1. AY010586 Genomic DNA. Translation: AAG37368.1. AY010587 Genomic DNA. Translation: AAG37369.1. AY010588 Genomic DNA. Translation: AAG37370.1. AY010589 Genomic DNA. Translation: AAG37371.1. AY010590 Genomic DNA. Translation: AAG37372.1. AY010591 Genomic DNA. Translation: AAG37373.1. AY010592 Genomic DNA. Translation: AAG37374.1. AF145666 mRNA. Translation: AAD38641.1.
RefSeq	NP_523594.1. NM_078870.2.
UniGene	Dm.29426.
3D structure databases
ProteinModelPortal	Q9V3R1.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24017N.
MINT	MINT-1010454.
STRING	Q9V3R1.
Proteomic databases
PRIDE	Q9V3R1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0081054; FBpp0080607; FBgn0011559.
GeneID	35096.
KEGG	dme:Dmel_CG7157.
NMPDR	fig|7227.3.peg.2822.
Organism-specific databases
CTD	35096.
FlyBase	FBgn0011559. Acp36DE.
Phylogenomic databases
eggNOG	euNOG20341.
GeneTree	EMGT00050000000917.
OrthoDB	EOG4VQ84V.
PhylomeDB	Q9V3R1.
Gene expression databases
Bgee	Q9V3R1.
GermOnline	CG7157. Drosophila melanogaster.
Family and domain databases
ProtoNet	Search...
Other
NextBio	791836.

Entry information

Entry name

A36DE_DROME

Accession

Primary (citable) accession number: Q9V3R1
Secondary accession number(s): O46198, Q9U9Y5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project