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Protein

26S proteasome non-ATPase regulatory subunit 1

Gene

Rpn2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.1 Publication

GO - Molecular functioni

  • enzyme regulator activity Source: InterPro
  • zinc ion binding Source: FlyBase

GO - Biological processi

  • cellular response to DNA damage stimulus Source: FlyBase
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase
  • regulation of protein catabolic process Source: InterPro

Enzyme and pathway databases

BRENDAi3.4.25.1. 1994.
ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-446652. Interleukin-1 family signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-6798695. Neutrophil degranulation.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-8948751. Regulation of PTEN stability and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 1
Alternative name(s):
26S proteasome regulatory complex subunit p110
26S proteasome regulatory subunit RPN2
Gene namesi
Name:Rpn2
ORF Names:CG11888
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0028692. Rpn2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738041 – 102026S proteasome non-ATPase regulatory subunit 1Add BLAST1020

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei291Phosphothreonine1 Publication1
Modified residuei298Phosphoserine1 Publication1
Modified residuei303Phosphoserine1 Publication1
Modified residuei305Phosphoserine1 Publication1
Modified residuei310Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9V3P6.
PRIDEiQ9V3P6.

PTM databases

iPTMnetiQ9V3P6.

Expressioni

Gene expression databases

BgeeiFBgn0028692.
ExpressionAtlasiQ9V3P6. differential.
GenevisibleiQ9V3P6. DM.

Interactioni

Protein-protein interaction databases

BioGridi68320. 39 interactors.
IntActiQ9V3P6. 8 interactors.
MINTiMINT-745147.
STRINGi7227.FBpp0303405.

Structurei

3D structure databases

ProteinModelPortaliQ9V3P6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati418 – 452PC 1Add BLAST35
Repeati456 – 489PC 2Add BLAST34
Repeati491 – 525PC 3Add BLAST35
Repeati526 – 560PC 4Add BLAST35
Repeati562 – 595PC 5Add BLAST34
Repeati596 – 631PC 6Add BLAST36
Repeati632 – 664PC 7Add BLAST33
Repeati666 – 701PC 8Add BLAST36
Repeati702 – 742PC 9Add BLAST41
Repeati745 – 777PC 10Add BLAST33

Sequence similaritiesi

Belongs to the proteasome subunit S1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2062. Eukaryota.
COG5116. LUCA.
GeneTreeiENSGT00550000074915.
InParanoidiQ9V3P6.
KOiK03032.
OrthoDBiEOG091G01IW.
PhylomeDBiQ9V3P6.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiView protein in InterPro
IPR016642. 26S_Psome_Rpn2.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002015. Proteasome/cyclosome_rpt.
IPR035266. PSMD1.
PANTHERiPTHR10943:SF5. PTHR10943:SF5. 1 hit.
PfamiView protein in Pfam
PF01851. PC_rep. 3 hits.
PIRSFiPIRSF015947. 26S_Psome_Rpn2. 1 hit.
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q9V3P6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLTSAAGII SLLDEPMPDL KVFALKKLDN IVDEFWPEIS ESIEKIEMLH
60 70 80 90 100
EDRSFPENKL AGMVASKVFY HLGSFEDALT YALGAGDLFD VNARNEYTET
110 120 130 140 150
IIAKCIDFYI AQRVEFIENP KEASVVDERL EGIVNRMIQR CLDDNQFRQA
160 170 180 190 200
LGIALETRRM DTFKDAIMKS DDVRGMLAYA YNVTMSLIQN RGFRNEVLRC
210 220 230 240 250
LVSLYRDLGV PDYVNMCQCL IFLEDPFAVA EMLDNLTRSS VETNNLMAYQ
260 270 280 290 300
IAFDLYESAT QEFLGNVLQH LKNTAPIPTA LPSTFKPQGT TSEDGAKSEG
310 320 330 340 350
DKSKSDEDIT EETPADDKVE RTIDSLNEVE KLHQKNIEKL ISILSGEVSI
360 370 380 390 400
DLQLQFLIRS NHADLQVLRG TKEAVRVSIC HTATVIANAF MHSGTTSDQF
410 420 430 440 450
LRDNLDWLAR ATNWAKLTAT ASLGVIHRGH EKDSLALMQS YLPKEAGPSS
460 470 480 490 500
GYSEGGALYA LGLIHANHGA NIIDYLLQQL KDAQNENVRH GGCLGLGLAG
510 520 530 540 550
MGTHRQDLYE QLKFNLYQDD AVTGEAAGIA MGMVMLGSKN AQAIEDMVSY
560 570 580 590 600
AQETQHEKIL RGLAVGISLT MFSRLEEADP LVTSLSSDKD PVLRRSGMYT
610 620 630 640 650
IAMAYNGTGS NKAIRKLLHV AVSDVNDDVR RAAVTAIGFI LFRSPEQCPS
660 670 680 690 700
VVSLLAESYN PHVRYGAAMA LGIACAGTGL REAIALLEPM VKFDPVNFVR
710 720 730 740 750
QGALIASAMI LIQHTDQSCP KSTFFRQLYA EVISNKHEDV MAKYGAILAQ
760 770 780 790 800
GIIDAGGRNA TLSLQSRTGH TNLQAVVGML AFTQYWYWFP LAHTLSLAFT
810 820 830 840 850
PTCVIGLNSD LKMPKMEYKS AAKPSLYAYP APLEEKKSEE REKVATAVLS
860 870 880 890 900
IAARQKRREN ADKKEDEKMD VDEDSKEGAA VKKDEEAKAD EKMVTDEKPK
910 920 930 940 950
KKDEKEKKKE EDKEKEAAGT SSEKDKDKEK DKKEKKEPEP TSEILQNPAR
960 970 980 990 1000
VLRQQLKVLS VIDGQSYEPL KDVTIGGIIV FQHTGKAEDQ ELVEPVAAFG
1010 1020
PMNDEEKEPE PPEPFEYIED
Length:1,020
Mass (Da):113,199
Last modified:May 1, 2000 - v1
Checksum:i8DE7AA5264742EB1
GO
Isoform B (identifier: Q9V3P6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     41-114: Missing.

Show »
Length:946
Mass (Da):104,810
Checksum:iD8C651BB1188974D
GO

Sequence cautioni

The sequence AAL39648 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti646E → G in AAN71353 (PubMed:12537569).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01161141 – 114Missing in isoform B. 1 PublicationAdd BLAST74

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145303 mRNA. Translation: AAF08384.1.
AF186594 Genomic DNA. Translation: AAG16726.2.
AF312231 mRNA. Translation: AAG33625.1.
AE014297 Genomic DNA. Translation: AAF56868.1.
AY069503 mRNA. Translation: AAL39648.1. Different initiation.
BT001598 mRNA. Translation: AAN71353.1.
BT044102 mRNA. Translation: ACH92167.1.
RefSeqiNP_001263058.1. NM_001276129.1. [Q9V3P6-1]
NP_651677.2. NM_143420.3. [Q9V3P6-1]
UniGeneiDm.6875.

Genome annotation databases

EnsemblMetazoaiFBtr0085385; FBpp0084754; FBgn0028692. [Q9V3P6-1]
FBtr0334532; FBpp0306599; FBgn0028692. [Q9V3P6-1]
GeneIDi43449.
KEGGidme:Dmel_CG11888.
UCSCiCG11888-RA. d. melanogaster. [Q9V3P6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSMD1_DROME
AccessioniPrimary (citable) accession number: Q9V3P6
Secondary accession number(s): B5RIH8
, Q8IGU4, Q8T075, Q9GSE5, Q9GU70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: May 1, 2000
Last modified: September 27, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families