Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peroxiredoxin 1

Gene

Jafrac1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. As a reducing substrate, thioredoxin 2 is preferred over thioredoxin 1.2 Publications

Miscellaneous

The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH rapidly reacts with Cys-168-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin (By similarity).By similarity
Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO3H) upon oxidative stress.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei47Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

  • thioredoxin peroxidase activity Source: UniProtKB

GO - Biological processi

  • cell adhesion Source: FlyBase
  • cell redox homeostasis Source: UniProtKB
  • cellular response to DNA damage stimulus Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • germ cell development Source: FlyBase
  • germ cell migration Source: FlyBase
  • hydrogen peroxide catabolic process Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • response to oxidative stress Source: FlyBase
  • response to starvation Source: FlyBase

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

ReactomeiR-DME-5628897. TP53 Regulates Metabolic Genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin 1 (EC:1.11.1.15)
Alternative name(s):
Cytosolic thioredoxin peroxidase
Short name:
DPx-4783
Short name:
DmTPx-1
Thioredoxin peroxidase
Gene namesi
Name:Jafrac1
Synonyms:TPX-1
ORF Names:CG1633
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0040309. Jafrac1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001350851 – 194Peroxiredoxin 1Add BLAST194

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47Interchain (with C-168); in linked formBy similarity
Disulfide bondi168Interchain (with C-47); in linked formBy similarity
Modified residuei193Phosphothreonine1 Publication1
Modified residuei194Phosphoserine1 Publication1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9V3P0.
PRIDEiQ9V3P0.

PTM databases

iPTMnetiQ9V3P0.

Expressioni

Developmental stagei

Highly expressed during embryogenesis, weakly expressed during larval stages.2 Publications

Gene expression databases

BgeeiFBgn0040309.
ExpressionAtlasiQ9V3P0. differential.
GenevisibleiQ9V3P0. DM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MtnBP119562EBI-82319,EBI-88468

Protein-protein interaction databases

BioGridi72802. 17 interactors.
DIPiDIP-17916N.
IntActiQ9V3P0. 16 interactors.
MINTiMINT-763596.
STRINGi7227.FBpp0073594.

Structurei

3D structure databases

ProteinModelPortaliQ9V3P0.
SMRiQ9V3P0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 160ThioredoxinPROSITE-ProRule annotationAdd BLAST159

Sequence similaritiesi

Belongs to the AhpC/TSA family.Curated

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
InParanoidiQ9V3P0.
KOiK13279.
OMAiCPANWEE.
OrthoDBiEOG091G0IE5.
PhylomeDBiQ9V3P0.

Family and domain databases

InterProiView protein in InterPro
IPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9V3P0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQLQKPAPA FAGTAVVNGV FKDIKLSDYK GKYLVLFFYP LDFTFVCPTE
60 70 80 90 100
IIAFSESAAE FRKINCEVIG CSTDSQFTHL AWINTPRKQG GLGSMDIPLL
110 120 130 140 150
ADKSMKVARD YGVLDEETGI PFRGLFIIDD KQNLRQITVN DLPVGRSVEE
160 170 180 190
TLRLVQAFQY TDKYGEVCPA NWKPGQKTMV ADPTKSKEYF ETTS
Length:194
Mass (Da):21,738
Last modified:May 1, 2000 - v1
Checksum:i93ED319BE144E8D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167098 mRNA. Translation: AAF42985.1.
AF321615 mRNA. Translation: AAK06770.1.
AF321616 mRNA. Translation: AAK06771.1.
AE014298 Genomic DNA. Translation: AAF48253.1.
AY070534 mRNA. Translation: AAL48005.1.
BT014630 mRNA. Translation: AAT27254.1.
RefSeqiNP_001285202.1. NM_001298273.1.
NP_001285203.1. NM_001298274.1.
NP_001285204.1. NM_001298275.1.
NP_477510.1. NM_058162.3.
NP_727689.1. NM_167359.2.
UniGeneiDm.3464.

Genome annotation databases

EnsemblMetazoaiFBtr0073763; FBpp0073594; FBgn0040309.
FBtr0073764; FBpp0073595; FBgn0040309.
FBtr0339699; FBpp0308756; FBgn0040309.
FBtr0339700; FBpp0308757; FBgn0040309.
FBtr0345161; FBpp0311371; FBgn0040309.
GeneIDi53578.
KEGGidme:Dmel_CG1633.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPRDX1_DROME
AccessioniPrimary (citable) accession number: Q9V3P0
Secondary accession number(s): Q0KHT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: May 1, 2000
Last modified: July 5, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families