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Protein

26S proteasome non-ATPase regulatory subunit 14

Gene

Rpn11

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi111Zinc; catalyticBy similarity1
Metal bindingi113Zinc; catalyticBy similarity1
Metal bindingi124Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase
  • protein deubiquitination Source: FlyBase

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.25.1. 1994.
ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-446652. Interleukin-1 signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-5689901. Metalloprotease DUBs.
R-DME-6798695. Neutrophil degranulation.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiM67.001.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 14 (EC:3.4.19.-)
Alternative name(s):
26S proteasome regulatory complex subunit p37B
26S proteasome regulatory subunit rpn11
Yippee-interacting protein 5
Gene namesi
Name:Rpn11
Synonyms:yip5
ORF Names:CG18174
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0028694. Rpn11.

Subcellular locationi

GO - Cellular componenti

  • microtubule associated complex Source: FlyBase
  • proteasome complex Source: FlyBase
  • proteasome regulatory particle Source: FlyBase
  • proteasome regulatory particle, lid subcomplex Source: FlyBase

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002139551 – 30826S proteasome non-ATPase regulatory subunit 14Add BLAST308

Proteomic databases

PaxDbiQ9V3H2.
PRIDEiQ9V3H2.

Expressioni

Gene expression databases

BgeeiFBgn0028694.
GenevisibleiQ9V3H2. DM.

Interactioni

Subunit structurei

Component of the 19S regulatory cap of the 26S proteasome.

Protein-protein interaction databases

BioGridi59913. 31 interactors.
IntActiQ9V3H2. 8 interactors.
MINTiMINT-819727.
STRINGi7227.FBpp0078664.

Structurei

3D structure databases

ProteinModelPortaliQ9V3H2.
SMRiQ9V3H2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 137MPNAdd BLAST114

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi111 – 124JAMM motifAdd BLAST14

Sequence similaritiesi

Belongs to the peptidase M67A family. PSMD14 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1555. Eukaryota.
COG1310. LUCA.
GeneTreeiENSGT00730000111116.
InParanoidiQ9V3H2.
KOiK03030.
OMAiQTGRPET.
OrthoDBiEOG091G0CMC.
PhylomeDBiQ9V3H2.

Family and domain databases

InterProiView protein in InterPro
IPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
PfamiView protein in Pfam
PF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
SMARTiView protein in SMART
SM00232. JAB_MPN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9V3H2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRLLRLGGA MPQAAPPTDA PVVDTAEQVY ISSLALLKML KHGRAGVPME
60 70 80 90 100
VMGLMLGEFV DDYTVQVIDV FAMPQTGTGV SVEAVDPVFQ AKMLDMLKQT
110 120 130 140 150
GRPEMVVGWY HSHPGFGCWL SGVDINTQQS FEALSERAVA VVVDPIQSVK
160 170 180 190 200
GKVVIDAFRL INPNMLVLGQ EPRQTTSNLG HLQKPSVQAL IHGLNRHYYS
210 220 230 240 250
ISINYRKNEL EQKMLLNLHK KSWKDGLTLS DYNEHCSINE DTVAEMLDLA
260 270 280 290 300
KNYNKSLEDE EKMTPEQLAI KNVGKQDPKR HLEEKVDKVM QNNIVQCLGA

MLDTIVFK
Length:308
Mass (Da):34,401
Last modified:May 1, 2000 - v1
Checksum:iCA43EEBD13B42A61
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144 – 145DP → YS in AAF27818 (Ref. 5) Curated2
Sequence conflicti249L → F in AAF27818 (Ref. 5) Curated1
Sequence conflicti268L → C AA sequence (PubMed:10893261).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145313 mRNA. Translation: AAF08394.1.
AE014134 Genomic DNA. Translation: AAF52215.1.
AY070977 mRNA. Translation: AAL48599.1.
AF195189 mRNA. Translation: AAF27818.1.
RefSeqiNP_608905.1. NM_135061.4.
UniGeneiDm.3343.

Genome annotation databases

EnsemblMetazoaiFBtr0079027; FBpp0078664; FBgn0028694.
GeneIDi33738.
KEGGidme:Dmel_CG18174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145313 mRNA. Translation: AAF08394.1.
AE014134 Genomic DNA. Translation: AAF52215.1.
AY070977 mRNA. Translation: AAL48599.1.
AF195189 mRNA. Translation: AAF27818.1.
RefSeqiNP_608905.1. NM_135061.4.
UniGeneiDm.3343.

3D structure databases

ProteinModelPortaliQ9V3H2.
SMRiQ9V3H2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59913. 31 interactors.
IntActiQ9V3H2. 8 interactors.
MINTiMINT-819727.
STRINGi7227.FBpp0078664.

Protein family/group databases

MEROPSiM67.001.

Proteomic databases

PaxDbiQ9V3H2.
PRIDEiQ9V3H2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079027; FBpp0078664; FBgn0028694.
GeneIDi33738.
KEGGidme:Dmel_CG18174.

Organism-specific databases

CTDi33738.
FlyBaseiFBgn0028694. Rpn11.

Phylogenomic databases

eggNOGiKOG1555. Eukaryota.
COG1310. LUCA.
GeneTreeiENSGT00730000111116.
InParanoidiQ9V3H2.
KOiK03030.
OMAiQTGRPET.
OrthoDBiEOG091G0CMC.
PhylomeDBiQ9V3H2.

Enzyme and pathway databases

BRENDAi3.4.25.1. 1994.
ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-446652. Interleukin-1 signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-5689901. Metalloprotease DUBs.
R-DME-6798695. Neutrophil degranulation.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii33738.
PROiPR:Q9V3H2.

Gene expression databases

BgeeiFBgn0028694.
GenevisibleiQ9V3H2. DM.

Family and domain databases

InterProiView protein in InterPro
IPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
PfamiView protein in Pfam
PF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
SMARTiView protein in SMART
SM00232. JAB_MPN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPSDE_DROME
AccessioniPrimary (citable) accession number: Q9V3H2
Secondary accession number(s): Q9NIU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: May 1, 2000
Last modified: June 7, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.