Reviewed, UniProtKB/Swiss-Prot Q9V3H2 (PSDE_DROME)

Names and origin

Protein names

Recommended name:
    26S proteasome non-ATPase regulatory subunit 14
    EC=3.1.2.15
Alternative name(s):
    26S proteasome regulatory subunit rpn11
    26S proteasome regulatory complex subunit p37B
    Yippee-interacting protein 5

Gene names

Name:	Rpn11
Synonyms:	yip5
ORF Names:	CG18174

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear By similarity.
Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Subunit structure	Component of the 19S regulatory cap of the 26S proteasome.
Sequence similarities	Belongs to the peptidase M67A family. PSMD14 subfamily. Contains 1 MPN (JAB/Mov34) domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Proteasome
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	proteasome regulatory particle Ref.1 Inferred from direct assay. Source: FlyBase
Molecular function	endopeptidase activity Ref.1 Inferred from direct assay. Source: FlyBase metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG17337	Q9V9H1	1	EBI-178674,EBI-144341
Mov34	P26270	1	EBI-178674,EBI-253612

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	26S proteasome non-ATPase regulatory subunit 14		PRO_0000213955
Regions
	Domain	24 – 137	114	MPN
	Motif	111 – 124	14	JAMM motif
Sites
	Metal binding	111	1	Zinc; catalytic By similarity
	Metal binding	113	1	Zinc; catalytic By similarity
	Metal binding	124	1	Zinc; catalytic By similarity
Experimental info
	Sequence conflict	144 – 145	2	DP → YS in AAF27818. Ref.5
	Sequence conflict	249	1	L → F in AAF27818. Ref.5
	Sequence conflict	268	1	L → C AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9V3H2-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: CA43EEBD13B42A61	FASTA	308	34,401
	10 20 30 40 50 60 MDRLLRLGGA MPQAAPPTDA PVVDTAEQVY ISSLALLKML KHGRAGVPME VMGLMLGEFV 70 80 90 100 110 120 DDYTVQVIDV FAMPQTGTGV SVEAVDPVFQ AKMLDMLKQT GRPEMVVGWY HSHPGFGCWL 130 140 150 160 170 180 SGVDINTQQS FEALSERAVA VVVDPIQSVK GKVVIDAFRL INPNMLVLGQ EPRQTTSNLG 190 200 210 220 230 240 HLQKPSVQAL IHGLNRHYYS ISINYRKNEL EQKMLLNLHK KSWKDGLTLS DYNEHCSINE 250 260 270 280 290 300 DTVAEMLDLA KNYNKSLEDE EKMTPEQLAI KNVGKQDPKR HLEEKVDKVM QNNIVQCLGA MLDTIVFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The regulatory complex of Drosophila melanogaster 26S proteasomes. Subunit composition and localization of a deubiquitylating enzyme." Hoelzl H., Kapelari B., Kellermann J., Seemueller E., Suemegi M., Udvardy A., Medalia O., Sperling J., Mueller S.A., Engel A., Baumeister W. J. Cell Biol. 150:119-130(2000) [PubMed: 10893261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 263-271, FUNCTION. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	Roxstrom-Lindquist K., Faye I. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 80-308. Tissue: Imaginal disk.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF145313 mRNA. Translation: AAF08394.1. AE014134 Genomic DNA. Translation: AAF52215.1. AY070977 mRNA. Translation: AAL48599.1. AF195189 mRNA. Translation: AAF27818.1.
RefSeq	NP_608905.1.
UniGene	Dm.3343
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9V3H2. 7 interactions.
STRING	Q9V3H2.
Protein family/group databases
MEROPS	M67.001.
Proteomic databases
PRIDE	Q9V3H2.
Genome annotation databases
Ensembl	FBtr0079027; FBpp0078664; FBgn0028694; Drosophila melanogaster. [Genome view]
GeneID	33738.
KEGG	dme:Dmel_CG18174.
NMPDR	fig|7227.3.peg.837.
Organism-specific databases
CTD	33738.
FlyBase	FBgn0028694. Rpn11.
Phylogenomic databases
HOGENOM	Q9V3H2.
OMA	QDPKKHL.
Gene expression databases
ArrayExpress	Q9V3H2.
Bgee	Q9V3H2.
GermOnline	CG18174. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000555. Mov34_MPN_PAD1. [Graphical view]
Pfam	PF01398. Mov34. 1 hit. [Graphical view]
ProDom	PD363422. Mov34-1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00232. JAB_MPN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	785010.

Entry information

Entry name

PSDE_DROME

Accession

Primary (citable) accession number: Q9V3H2
Secondary accession number(s): Q9NIU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project