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Protein

26S proteasome non-ATPase regulatory subunit 14

Gene

Rpn11

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi111Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi113Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi124Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase
  • protein deubiquitination Source: FlyBase

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.25.1 1994
ReactomeiR-DME-1169091 Activation of NF-kappaB in B cells
R-DME-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-DME-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-DME-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-DME-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-DME-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-DME-187577 SCF(Skp2)-mediated degradation of p27/p21
R-DME-195253 Degradation of beta-catenin by the destruction complex
R-DME-202424 Downstream TCR signaling
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209406 Degradation of NF-kappa-B inhibitor, CACT
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-216167 Nuclear CI is degraded
R-DME-2871837 FCERI mediated NF-kB activation
R-DME-432395 Degradation of TIM
R-DME-432524 Degradation of PER
R-DME-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-DME-4608870 Asymmetric localization of PCP proteins
R-DME-4641257 Degradation of AXIN
R-DME-4641258 Degradation of DVL
R-DME-5358346 Hedgehog ligand biogenesis
R-DME-538848 Degradation of CLK
R-DME-538864 Degradation of CRY
R-DME-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-DME-5607764 CLEC7A (Dectin-1) signaling
R-DME-5610785 GLI3 is processed to GLI3R by the proteasome
R-DME-5632684 Hedgehog 'on' state
R-DME-5658442 Regulation of RAS by GAPs
R-DME-5676590 NIK-->noncanonical NF-kB signaling
R-DME-5689603 UCH proteinases
R-DME-5689880 Ub-specific processing proteases
R-DME-5689901 Metalloprotease DUBs
R-DME-6798695 Neutrophil degranulation
R-DME-68949 Orc1 removal from chromatin
R-DME-69017 CDK-mediated phosphorylation and removal of Cdc6
R-DME-69229 Ubiquitin-dependent degradation of Cyclin D1
R-DME-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-DME-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-DME-8939902 Regulation of RUNX2 expression and activity
R-DME-8941858 Regulation of RUNX3 expression and activity
R-DME-8948751 Regulation of PTEN stability and activity
R-DME-9020702 Interleukin-1 signaling
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiM67.001

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 14 (EC:3.4.19.-)
Alternative name(s):
26S proteasome regulatory complex subunit p37B
26S proteasome regulatory subunit rpn11
Yippee-interacting protein 5
Gene namesi
Name:Rpn11
Synonyms:yip5
ORF Names:CG18174
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0028694 Rpn11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002139551 – 30826S proteasome non-ATPase regulatory subunit 14Add BLAST308

Proteomic databases

PaxDbiQ9V3H2
PRIDEiQ9V3H2

Expressioni

Gene expression databases

BgeeiFBgn0028694
GenevisibleiQ9V3H2 DM

Interactioni

Subunit structurei

Component of the 19S regulatory cap of the 26S proteasome.

Protein-protein interaction databases

BioGridi59913, 32 interactors
IntActiQ9V3H2, 8 interactors
STRINGi7227.FBpp0078664

Structurei

3D structure databases

ProteinModelPortaliQ9V3H2
SMRiQ9V3H2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 164MPNPROSITE-ProRule annotationAdd BLAST136

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi111 – 124JAMM motifPROSITE-ProRule annotationAdd BLAST14

Sequence similaritiesi

Belongs to the peptidase M67A family. PSMD14 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1555 Eukaryota
COG1310 LUCA
GeneTreeiENSGT00730000111116
InParanoidiQ9V3H2
KOiK03030
OMAiQTGRPET
OrthoDBiEOG091G0CMC
PhylomeDBiQ9V3H2

Family and domain databases

InterProiView protein in InterPro
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
IPR035299 PSMD14
IPR024969 Rpn11/EIF3F_C
PANTHERiPTHR10410:SF13 PTHR10410:SF13, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
PF13012 MitMem_reg, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit

Sequencei

Sequence statusi: Complete.

Q9V3H2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRLLRLGGA MPQAAPPTDA PVVDTAEQVY ISSLALLKML KHGRAGVPME
60 70 80 90 100
VMGLMLGEFV DDYTVQVIDV FAMPQTGTGV SVEAVDPVFQ AKMLDMLKQT
110 120 130 140 150
GRPEMVVGWY HSHPGFGCWL SGVDINTQQS FEALSERAVA VVVDPIQSVK
160 170 180 190 200
GKVVIDAFRL INPNMLVLGQ EPRQTTSNLG HLQKPSVQAL IHGLNRHYYS
210 220 230 240 250
ISINYRKNEL EQKMLLNLHK KSWKDGLTLS DYNEHCSINE DTVAEMLDLA
260 270 280 290 300
KNYNKSLEDE EKMTPEQLAI KNVGKQDPKR HLEEKVDKVM QNNIVQCLGA

MLDTIVFK
Length:308
Mass (Da):34,401
Last modified:May 1, 2000 - v1
Checksum:iCA43EEBD13B42A61
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144 – 145DP → YS in AAF27818 (Ref. 5) Curated2
Sequence conflicti249L → F in AAF27818 (Ref. 5) Curated1
Sequence conflicti268L → C AA sequence (PubMed:10893261).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145313 mRNA Translation: AAF08394.1
AE014134 Genomic DNA Translation: AAF52215.1
AY070977 mRNA Translation: AAL48599.1
AF195189 mRNA Translation: AAF27818.1
RefSeqiNP_608905.1, NM_135061.4
UniGeneiDm.3343

Genome annotation databases

EnsemblMetazoaiFBtr0079027; FBpp0078664; FBgn0028694
GeneIDi33738
KEGGidme:Dmel_CG18174

Similar proteinsi

Entry informationi

Entry nameiPSDE_DROME
AccessioniPrimary (citable) accession number: Q9V3H2
Secondary accession number(s): Q9NIU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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