Reviewed, UniProtKB/Swiss-Prot Q9V3C0 (DDX41_DROME)

Names and origin

Protein names

Recommended name:
    ATP-dependent RNA helicase abstrakt
      Short name=DEAD box protein abstrakt
    EC=3.6.1.-

Gene names

Name:	abs
ORF Names:	CG14637

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ATP-dependent RNA helicase. Is essential for the directed and fasciculated early outgrowth of the bolwig nerves, as well as for its navigation at later stages. Is required during post-transcriptional gene expression. Plays a role during morphogenetic process, apoptosis and the establishment of cell polarity.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the DEAD box helicase family. DDX41 subfamily. Contains 1 CCHC-type zinc finger. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VB74	1	EBI-134927,EBI-169306

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	ATP-dependent RNA helicase abstrakt		PRO_0000054974
Regions
	Domain	208 – 392	185	Helicase ATP-binding
	Domain	403 – 563	161	Helicase C-terminal
	Nucleotide binding	221 – 228	8	ATP By similarity
	Zinc finger	577 – 594	18	CCHC-type
	Motif	177 – 205	29	Q motif
	Motif	340 – 343	4	DEAD box
Amino acid modifications
	Modified residue	11	1	Phosphoserine Ref.6
	Modified residue	13	1	Phosphoserine Ref.6
	Modified residue	14	1	Phosphoserine Ref.6
	Modified residue	56	1	Phosphoserine Ref.6
	Modified residue	57	1	Phosphoserine Ref.6
	Modified residue	58	1	Phosphoserine Ref.6
	Modified residue	66	1	Phosphoserine Ref.6
Natural variations
	Natural variant	241	1	E → K in allele 14B; temperature sensitive.
	Natural variant	431	1	V → M in allele 33B; temperature sensitive.
Experimental info
	Sequence conflict	5	1	K → Q in AAF04040. Ref.2
	Sequence conflict	26 – 30	5	Missing Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9V3C0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F148D277A1FD0BEC	FASTA	619	69,488
	10 20 30 40 50 60 MAHVKRYRRS SKSSEEGDLD NEDYVPYVPV KERKKQHMIK LGRIVQLVSE TAQPKSSSEN 70 80 90 100 110 120 ENEDDSQGAH DVETWGRKYN ISLLDQHTEL KKIAEAKKLS AVEKQLREEE KIMESIAQQK 130 140 150 160 170 180 ALMGVAELAK GIQYEQPIKT AWKPPRYIRE MSEEEREAVR HELRILVEGE TPSPPIRSFR 190 200 210 220 230 240 EMKFPKGILN GLAAKGIKNP TPIQVQGLPT VLAGRDLIGI AFTGSGKTLV FVLPVIMFAL 250 260 270 280 290 300 EQEYSLPFER NEGPYGLIIC PSRELAKQTH EIIQHYSKHL QACGMPEIRS CLAMGGLPVS 310 320 330 340 350 360 EALDVISRGV HIVVATPGRL MDMLDKKILT LDMCRYLCMD EADRMIDMGF EEDVRTIFSF 370 380 390 400 410 420 FKGQRQTLLF SATMPKKIQN FARSALVKPV TINVGRAGAA SMNVTQQVEY VKQEAKVVYL 430 440 450 460 470 480 LDCLQKTAPP VLIFAEKKQD VDCIHEYLLL KGVEAVAIHG GKDQEERSRA VDAYRVGKKD 490 500 510 520 530 540 VLVATDVASK GLDFPNVQHV INYDMPDDIE NYVHRIGRTG RSNTKGLATT LINKTTEQSV 550 560 570 580 590 600 LLDLKHLLIE GKQEVPDFLD ELAPETEHQH LDLGDSHGCT YCGGLGHRIT ECPKLEAVQN 610 KQASNIGRRD YLSNTAADY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila gene abstrakt, required for visual system development, encodes a putative RNA helicase of the DEAD box protein family." Schmucker D., Vorbrueggen G., Yeghiayan P., Fan H.Q., Jaeckle H., Gaul U. Mech. Dev. 91:189-196(2000) [PubMed: 10704843] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Developmental and cell biological functions of the Drosophila DEAD-box protein abstrakt." Irion U., Leptin M. Curr. Biol. 9:1373-1381(1999) [PubMed: 10607561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS 14B AND 33B.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11; SER-13; SER-14; SER-56; SER-57; SER-58 AND SER-66, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF212866 mRNA. Translation: AAF19985.1. AF187729 Genomic DNA. Translation: AAF04040.1. AE014297 Genomic DNA. Translation: AAF52165.1. AY051752 mRNA. Translation: AAK93176.1.
RefSeq	NP_524220.1.
UniGene	Dm.3300
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:21861N.
IntAct	Q9V3C0. 5 interactions.
STRING	Q9V3C0.
Proteomic databases
PRIDE	Q9V3C0.
Genome annotation databases
Ensembl	FBtr0078967; FBpp0078606; FBgn0015331; Drosophila melanogaster. [Genome view]
GeneID	40530.
KEGG	dme:Dmel_CG14637.
UCSC	CG14637-RA. d. melanogaster.
Organism-specific databases
CTD	40530.
FlyBase	FBgn0015331. abs.
Phylogenomic databases
HOGENOM	Q9V3C0.
OMA	MGFEEDV
OrthoDB	EOG98WC7V
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-009859-MON.
Gene expression databases
Bgee	Q9V3C0.
GermOnline	CG14637. Drosophila melanogaster.
Family and domain databases
InterPro	IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR014014. RNA_helicase_DEAD_Q_motif. IPR001878. Znf_CCHC. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. SM00343. ZnF_C2HC. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. False negative. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. PS50158. ZF_CCHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	819209.

Entry information

Entry name

DDX41_DROME

Accession

Primary (citable) accession number: Q9V3C0
Secondary accession number(s): Q9U6D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	November 24, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project