Reviewed, UniProtKB/Swiss-Prot Q9V3B7 (PGSC1_DROME)

Names and origin

Protein names

Recommended name:
    Peptidoglycan-recognition protein SC1a/b
    EC=3.5.1.28

Gene names

Name:	PGRP-SC1a
ORF Names:	CG14746
AND
Name:	PGRP-SC1b
ORF Names:	CG8577

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN). Plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Has no direct bacteriolytic activity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. Ref.7
Cofactor	Zinc By similarity.
Subcellular location	Secreted Potential.
Tissue specificity	Constitutively expressed at high level in gut, in addition to the induced expression in fat body. Ref.1
Induction	Up-regulated by PGN from B.subtilis. Ref.1
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Ontologies

Keywords
Biological process	Immune response Innate immunity
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	innate immune response Ref.1 Non-traceable author statement. Source: UniProtKB peptidoglycan catabolic process Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	extracellular region Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Ref.7 Inferred from direct assay. Source: FlyBase peptidoglycan binding Ref.1 Non-traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9W0H1	1	EBI-109541,EBI-143367

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 185	164	Peptidoglycan-recognition protein SC1a/b		PRO_0000023910
Sites
	Metal binding	52	1	Zinc By similarity
	Metal binding	86	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity
	Metal binding	168	1	Zinc By similarity
Amino acid modifications
	Disulfide bond	58 ↔ 64		By similarity
Natural variations
	Natural variant	6	1	A → T in PGRP-SC1a; strain: KY038 and in PGRP-SC1b; strain: KY024 and KY038.
	Natural variant	13	1	V → I in PGRP-SC1b; strain: KY024.
Experimental info
	Mutagenesis	168	1	C → A: Abolishes enzyme activity but retains PGN-binding. Ref.7
	Sequence conflict	178	1	R → P in AAL28193. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9V3B7-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F23F8D80A33541AC	FASTA	185	20,395
	10 20 30 40 50 60 MVSKVALLLA VLVCSQYMAQ GVYVVSKAEW GGRGAKWTVG LGNYLSYAII HHTAGSYCET 70 80 90 100 110 120 RAQCNAVLQS VQNYHMDSLG WPDIGYNFLI GGDGNVYEGR GWNNMGAHAA EWNPYSIGIS 130 140 150 160 170 180 FLGNYNWDTL EPNMISAAQQ LLNDAVNRGQ LSSGYILYGH RQVSATECPG THIWNEIRGW SHWSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster." Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D. Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed: 11106397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (PGRP-SC1B), PGN-BINDING, TISSUE SPECIFICITY, INDUCTION.
[2]	"The evolution of parasite recognition genes in the innate immune system: purifying selection on Drosophila melanogaster peptidoglycan recognition proteins." Jiggins F.M., Hurst G.D.D. J. Mol. Evol. 57:598-605(2003) [PubMed: 14738318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (PGRP-SC1A AND PGRP-SC1B). Strain: DI7, Draveil, KY024, KY038, Loua, Monty5, P.bourg, S30, Tahiti, Texas and ZW141.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (PGRP-SC1A AND PGRP-SC1B). Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (PGRP-SC1B). Strain: Berkeley. Tissue: Head.
[6]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (PGRP-SC1B). Strain: Berkeley.
[7]	"A scavenger function for a Drosophila peptidoglycan recognition protein." Mellroth P., Karlsson J., Steiner H. J. Biol. Chem. 278:7059-7064(2003) [PubMed: 12496260] [Abstract] Cited for: ENZYME ACTIVITY (PGRP-SC1B), PGN-BINDING, MUTAGENESIS OF CYS-168.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF207542 mRNA. Translation: AAG23736.1. AJ556588 Genomic DNA. Translation: CAD89153.1. AJ556589 Genomic DNA. Translation: CAD89154.1. AJ556590 Genomic DNA. Translation: CAD89155.1. AJ556591 Genomic DNA. Translation: CAD89156.1. AJ556592 Genomic DNA. Translation: CAD89157.1. AJ556593 Genomic DNA. Translation: CAD89158.1. AJ556594 Genomic DNA. Translation: CAD89159.1. AJ556595 Genomic DNA. Translation: CAD89160.1. AJ556596 Genomic DNA. Translation: CAD89161.1. AJ556597 Genomic DNA. Translation: CAD89162.1. AJ556598 Genomic DNA. Translation: CAD89163.1. AJ556599 Genomic DNA. Translation: CAD89164.1. AJ556600 Genomic DNA. Translation: CAD89165.1. AJ556601 Genomic DNA. Translation: CAD89166.1. AJ556602 Genomic DNA. Translation: CAD89167.1. AJ556603 Genomic DNA. Translation: CAD89168.1. AJ556604 Genomic DNA. Translation: CAD89169.1. AJ556605 Genomic DNA. Translation: CAD89170.1. AJ556606 Genomic DNA. Translation: CAD89171.1. AJ556607 Genomic DNA. Translation: CAD89172.1. AJ556608 Genomic DNA. Translation: CAD89173.1. AJ556609 Genomic DNA. Translation: CAD89174.1. AE013599 Genomic DNA. Translation: AAF59052.1. AE013599 Genomic DNA. Translation: AAF59054.1. AY060645 mRNA. Translation: AAL28193.2. Different initiation. BT044352 mRNA. Translation: ACH92417.1. Different initiation.
RefSeq	NP_610407.1. NP_610409.1.
UniGene	Dm.3372 Dm.36623
3D structure databases
HSSP	HSSP built from PDB template 1OHT based on UniProtKB Q9VGN3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9V3B7. 1 interaction.
Proteomic databases
PRIDE	Q9V3B7.
Genome annotation databases
Ensembl	FBtr0088707; FBpp0087786; FBgn0043576; Drosophila melanogaster. [Genome view] FBtr0088708; FBpp0087787; FBgn0033327; Drosophila melanogaster. [Genome view]
GeneID	35859. 35861.
KEGG	dme:Dmel_CG14746. dme:Dmel_CG8577.
UCSC	CG14746-RA. d. melanogaster.
Organism-specific databases
CTD	35859. 35861.
FlyBase	FBgn0043576. PGRP-SC1a. FBgn0033327. PGRP-SC1b.
Phylogenomic databases
HOGENOM	Q9V3B7.
OMA	THIWNEI.
Enzyme and pathway databases
BRENDA	3.5.1.28. 48.
Gene expression databases
GermOnline	CG14746. Drosophila melanogaster. CG8577. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002502. Amidase_2. IPR017331. Peptidoglycan_recognition. IPR015510. PGRP. IPR006619. PGRP_met/bac. [Graphical view]
Gene3D	G3DSA:3.40.80.10. Amidase_2. 1 hit.
PANTHER	PTHR11022. PGRPs. 1 hit.
Pfam	PF01510. Amidase_2. 1 hit. [Graphical view]
PIRSF	PIRSF037945. PGRPs. 1 hit.
SMART	SM00644. Ami_2. 1 hit. SM00701. PGRP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	795548.

Entry information

Entry name

PGSC1_DROME

Accession

Primary (citable) accession number: Q9V3B7
Secondary accession number(s): B5RJ78 , Q70PU9, Q70PV0, Q95SQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project