Q9V3B4 (BG4_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Death domain-containing adapter protein BG4

Alternative name(s):
FAS-associated death domain-containing protein
Short name=dFADD

Gene names

Name:	BG4
ORF Names:	CG12297

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the IMD signaling pathway and is required for the host defense against Gram-negative bacteria. BG4 interacts with Dredd, it promotes cleavage of Dredd and is necessary and sufficient for enhancing Dredd-induced apoptosis. Ref.1 Ref.6
Subunit structure	N-terminus interacts with Dredd. Interacts with imd. Ref.1 Ref.6
Subcellular location	Cytoplasm Ref.1.
Developmental stage	Expressed between 3-12 hours of embryonic development. Ref.1
Sequence similarities	Contains 1 death domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of pro-apoptotic gene products Inferred from direct assay Ref.1. Source: FlyBase defense response to Gram-negative bacterium Inferred from mutant phenotype Ref.6. Source: UniProtKB immune response Inferred from mutant phenotype. Source: FlyBase peptidoglycan recognition protein signaling pathway Inferred from genetic interaction Ref.6. Source: FlyBase positive regulation of antibacterial peptide biosynthetic process Traceable author statement. Source: FlyBase sperm individualization Inferred from mutant phenotype. Source: FlyBase
Cellular component	cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	CARD domain binding Inferred from physical interaction Ref.1. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Death domain-containing adapter protein BG4		PRO_0000271422
Regions
	Domain	151 – 237	87	Death
	Region	1 – 105	105	Death-inducing Ref.1
Natural variations
	Natural variant	71	1	I → M in strain: G130 and G140. Ref.5
	Natural variant	87	1	K → P in strain: G125, G130 and G140. Ref.5
	Natural variant	135	1	T → P in strain: G02, G125, G130 and G140. Ref.5
	Natural variant	143	1	P → T in strain: G02, G130 and G140. Ref.5
	Natural variant	149	1	H → Q in strain: G125. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9V3B4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F43CFAA546C3FCD9	FASTA	239	27,421
	10 20 30 40 50 60 MTAGRHWSYD SLKQIAIDGC TENVEQLKLI FVEEIGSRRR SDCIRTIEDL IDCLERADEL 70 80 90 100 110 120 SEYNVEPLRR ISGNMPQLIE ALSAYTKPEN ILGHPVNLYQ ELRLAEELRQ QLRIAPASQN 130 140 150 160 170 180 AQPSVSELAA AVPPTAIQNY ATPAAFTDHK RTMVFKKISE ELGRYWRRLG RSAGIGEGQM 190 200 210 220 230 DTIEERYPHD LKSQILRLLQ LIEEDDCHDP KHFLLRLCRA LGDCGRNDLR KRVEQIMSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"dFADD, a novel death domain-containing adapter protein for the Drosophila caspase DREDD." Hu S., Yang X. J. Biol. Chem. 275:30761-30764(2000) [PubMed: 10934188] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH DREDD, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[2]	"BG4_a potential Drosophila homologue of FAS-associating death-domain containing protein (FADD)." Zhou L., Steller H. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A screen for immunity genes evolving under positive selection in Drosophila." Jiggins F.M., Kim K.W. J. Evol. Biol. 20:965-970(2007) [PubMed: 17465907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-201, VARIANTS MET-71; PRO-87; PRO-135; THR-143 AND GLN-149. Strain: G02, G125, G130 and G140.
[6]	"The Drosophila immune defense against Gram-negative infection requires the death protein dFADD." Naitza S., Rosse C., Kappler C., Georgel P., Belvin M., Gubb D., Camonis J., Hoffmann J.A., Reichhart J.-M. Immunity 17:575-581(2002) [PubMed: 12433364] [Abstract] Cited for: FUNCTION, INTERACTION WITH IMD.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF295103 mRNA. Translation: AAG22535.1. AF222005 mRNA. Translation: AAF44325.1. AE014297 Genomic DNA. Translation: AAF55950.1. AM412815 Genomic DNA. Translation: CAL85438.1. AM412816 Genomic DNA. Translation: CAL85439.1. AM412817 Genomic DNA. Translation: CAL85440.1. AM412818 Genomic DNA. Translation: CAL85441.1.
RefSeq	NP_651006.1. NM_142749.2.
UniGene	Dm.1273.
3D structure databases
HSSP	HSSP built from PDB template 1FAD based on UniProtKB Q61160.
ProteinModelPortal	Q9V3B4.
SMR	Q9V3B4. Positions 10-91, 147-237.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-18912N.
IntAct	Q9V3B4. 6 interactions.
MINT	MINT-142235.
STRING	Q9V3B4.
Proteomic databases
PRIDE	Q9V3B4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0084176; FBpp0083574; FBgn0038928.
GeneID	42594.
KEGG	dme:Dmel_CG12297.
UCSC	CG12297-RA. d. melanogaster.
Organism-specific databases
CTD	42594.
FlyBase	FBgn0038928. BG4.
Phylogenomic databases
eggNOG	inNOG11852.
GeneTree	EMGT00050000016483.
InParanoid	Q9V3B4.
OMA	VEQIMSH.
OrthoDB	EOG4B2RDG.
PhylomeDB	Q9V3B4.
Gene expression databases
Bgee	Q9V3B4.
Family and domain databases
InterPro	IPR000488. Death. IPR011029. DEATH-like. [Graphical view]
Gene3D	G3DSA:1.10.533.10. DEATH_like. 2 hits.
Pfam	PF00531. Death. 1 hit. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit.
PROSITE	PS50017. DEATH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	829592.

Entry information

Entry name

BG4_DROME

Accession

Primary (citable) accession number: Q9V3B4
Secondary accession number(s): A4V9R5, A4V9R6, A4V9R7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project