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Protein

Vacuolar protein sorting-associated protein 28 homolog

Gene

Vps28

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process.By similarity

GO - Biological processi

  • actin cytoskeleton organization Source: FlyBase
  • autophagosome maturation Source: FlyBase
  • autophagy Source: FlyBase
  • compound eye development Source: FlyBase
  • embryo development ending in birth or egg hatching Source: FlyBase
  • endosome transport via multivesicular body sorting pathway Source: FlyBase
  • negative regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
  • negative regulation of growth of symbiont in host Source: FlyBase
  • neuron remodeling Source: FlyBase
  • protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
  • regulation of multivesicular body size Source: FlyBase
  • regulation of Notch signaling pathway Source: FlyBase
  • sperm individualization Source: FlyBase
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: FlyBase

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-DME-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 28 homolog
Alternative name(s):
ESCRT-I complex subunit VPS28
Gene namesi
Name:Vps28
Synonyms:l(2)k16503
ORF Names:CG12770
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0021814 Vps28

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome

Pathology & Biotechi

Disruption phenotypei

Death at the transition from the first to second instar. Morphological changes in MVBs and developmental defects in the compound eye. However, for the ligands and receptors tested, changes in their cell surface levels or their delivery to lysosomes have not been detected. In early embryos and during sperm individualization defects in actin cytoskeleton organization ocurr.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001209541 – 210Vacuolar protein sorting-associated protein 28 homologAdd BLAST210

Proteomic databases

PaxDbiQ9V359
PRIDEiQ9V359

Expressioni

Gene expression databases

BgeeiFBgn0021814
ExpressionAtlasiQ9V359 differential
GenevisibleiQ9V359 DM

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I).By similarity

Protein-protein interaction databases

BioGridi707939 interactors.
DIPiDIP-20897N
IntActiQ9V359 9 interactors.
STRINGi7227.FBpp0302051

Structurei

3D structure databases

ProteinModelPortaliQ9V359
SMRiQ9V359
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 108VPS28 N-terminalPROSITE-ProRule annotationAdd BLAST108
Domaini112 – 208VPS28 C-terminalPROSITE-ProRule annotationAdd BLAST97

Sequence similaritiesi

Belongs to the VPS28 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3284 Eukaryota
ENOG4111IQ4 LUCA
GeneTreeiENSGT00390000007486
InParanoidiQ9V359
KOiK12184
OMAiKYRLDCP
OrthoDBiEOG091G0LT0
PhylomeDBiQ9V359

Family and domain databases

Gene3Di1.20.120.11301 hit
1.20.1440.2001 hit
InterProiView protein in InterPro
IPR037202 ESCRT_assembly_dom
IPR007143 Vps28
IPR017899 VPS28_C
IPR037206 VPS28_C_sf
IPR017898 VPS28_N
IPR038358 VPS28_N_sf
PANTHERiPTHR12937 PTHR12937, 1 hit
PfamiView protein in Pfam
PF03997 VPS28, 1 hit
PIRSFiPIRSF017535 VPS28, 1 hit
SUPFAMiSSF140111 SSF140111, 1 hit
SSF140427 SSF140427, 1 hit
PROSITEiView protein in PROSITE
PS51310 VPS28_C, 1 hit
PS51313 VPS28_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q9V359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQSPELYE EVKLFRNARE REKYDNMADL YAIINTIQQL EKAYIRDCIT
60 70 80 90 100
PQEYTAACSK YLVQYKVAFK QVQCDEFPSV ETFVKKFRLD CPAALERIRE
110 120 130 140 150
DRPITIRDDK GNTSKCIAEI VSLFITIMDK LRLQINTMDA LQPDVKDLAD
160 170 180 190 200
NMNRLSLIPE DFDAKLKVEK WLGSLNEMQA SDELSEGQVR QFLFDLESAY
210
ADFNKLLHSQ
Length:210
Mass (Da):24,512
Last modified:May 1, 2000 - v1
Checksum:i843DB9820ADE9D96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA Translation: AAF59143.1
AY060620 mRNA Translation: AAL28168.1
RefSeqiNP_001260796.1, NM_001273867.2
NP_652053.1, NM_143796.4
UniGeneiDm.3935

Genome annotation databases

EnsemblMetazoaiFBtr0088825; FBpp0087901; FBgn0021814
FBtr0310370; FBpp0302051; FBgn0021814
GeneIDi47408
KEGGidme:Dmel_CG12770
UCSCiCG12770-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiVPS28_DROME
AccessioniPrimary (citable) accession number: Q9V359
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome