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Protein

Vacuolar protein sorting-associated protein 28 homolog

Gene

Vps28

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process.By similarity

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: FlyBase
  • autophagosome maturation Source: FlyBase
  • autophagy Source: FlyBase
  • compound eye development Source: FlyBase
  • embryo development ending in birth or egg hatching Source: FlyBase
  • endosome transport via multivesicular body sorting pathway Source: FlyBase
  • epithelium development Source: FlyBase
  • germarium-derived egg chamber formation Source: FlyBase
  • negative regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
  • negative regulation of growth of symbiont in host Source: FlyBase
  • neuron remodeling Source: FlyBase
  • ovarian follicle cell stalk formation Source: FlyBase
  • protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
  • regulation of multivesicular body size Source: FlyBase
  • regulation of Notch signaling pathway Source: FlyBase
  • sperm individualization Source: FlyBase
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-DME-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 28 homolog
Alternative name(s):
ESCRT-I complex subunit VPS28
Gene namesi
Name:Vps28
Synonyms:l(2)k16503
ORF Names:CG12770
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0021814. Vps28.

Subcellular locationi

GO - Cellular componenti

  • ESCRT I complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Endosome

Pathology & Biotechi

Disruption phenotypei

Death at the transition from the first to second instar. Morphological changes in MVBs and developmental defects in the compound eye. However, for the ligands and receptors tested, changes in their cell surface levels or their delivery to lysosomes have not been detected. In early embryos and during sperm individualization defects in actin cytoskeleton organization ocurr.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001209541 – 210Vacuolar protein sorting-associated protein 28 homologAdd BLAST210

Proteomic databases

PaxDbiQ9V359.
PRIDEiQ9V359.

Expressioni

Gene expression databases

BgeeiFBgn0021814.
ExpressionAtlasiQ9V359. baseline.
GenevisibleiQ9V359. DM.

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi70793. 5 interactors.
DIPiDIP-20897N.
IntActiQ9V359. 1 interactor.
MINTiMINT-1000470.
STRINGi7227.FBpp0302051.

Structurei

3D structure databases

ProteinModelPortaliQ9V359.
SMRiQ9V359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 108VPS28 N-terminalPROSITE-ProRule annotationAdd BLAST108
Domaini112 – 208VPS28 C-terminalPROSITE-ProRule annotationAdd BLAST97

Sequence similaritiesi

Belongs to the VPS28 family.PROSITE-ProRule annotation
Contains 1 VPS28 C-terminal domain.PROSITE-ProRule annotation
Contains 1 VPS28 N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3284. Eukaryota.
ENOG4111IQ4. LUCA.
GeneTreeiENSGT00390000007486.
InParanoidiQ9V359.
KOiK12184.
OMAiKYRLDCP.
OrthoDBiEOG091G0LT0.
PhylomeDBiQ9V359.

Family and domain databases

InterProiIPR007143. VPS28.
IPR017899. VPS28_C.
IPR017898. VPS28_N.
[Graphical view]
PANTHERiPTHR12937. PTHR12937. 1 hit.
PfamiPF03997. VPS28. 1 hit.
[Graphical view]
PIRSFiPIRSF017535. VPS28. 1 hit.
PROSITEiPS51310. VPS28_C. 1 hit.
PS51313. VPS28_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9V359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQSPELYE EVKLFRNARE REKYDNMADL YAIINTIQQL EKAYIRDCIT
60 70 80 90 100
PQEYTAACSK YLVQYKVAFK QVQCDEFPSV ETFVKKFRLD CPAALERIRE
110 120 130 140 150
DRPITIRDDK GNTSKCIAEI VSLFITIMDK LRLQINTMDA LQPDVKDLAD
160 170 180 190 200
NMNRLSLIPE DFDAKLKVEK WLGSLNEMQA SDELSEGQVR QFLFDLESAY
210
ADFNKLLHSQ
Length:210
Mass (Da):24,512
Last modified:May 1, 2000 - v1
Checksum:i843DB9820ADE9D96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF59143.1.
AY060620 mRNA. Translation: AAL28168.1.
RefSeqiNP_001260796.1. NM_001273867.2.
NP_652053.1. NM_143796.4.
UniGeneiDm.3935.

Genome annotation databases

EnsemblMetazoaiFBtr0088825; FBpp0087901; FBgn0021814.
FBtr0310370; FBpp0302051; FBgn0021814.
GeneIDi47408.
KEGGidme:Dmel_CG12770.
UCSCiCG12770-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF59143.1.
AY060620 mRNA. Translation: AAL28168.1.
RefSeqiNP_001260796.1. NM_001273867.2.
NP_652053.1. NM_143796.4.
UniGeneiDm.3935.

3D structure databases

ProteinModelPortaliQ9V359.
SMRiQ9V359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi70793. 5 interactors.
DIPiDIP-20897N.
IntActiQ9V359. 1 interactor.
MINTiMINT-1000470.
STRINGi7227.FBpp0302051.

Proteomic databases

PaxDbiQ9V359.
PRIDEiQ9V359.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088825; FBpp0087901; FBgn0021814.
FBtr0310370; FBpp0302051; FBgn0021814.
GeneIDi47408.
KEGGidme:Dmel_CG12770.
UCSCiCG12770-RA. d. melanogaster.

Organism-specific databases

CTDi51160.
FlyBaseiFBgn0021814. Vps28.

Phylogenomic databases

eggNOGiKOG3284. Eukaryota.
ENOG4111IQ4. LUCA.
GeneTreeiENSGT00390000007486.
InParanoidiQ9V359.
KOiK12184.
OMAiKYRLDCP.
OrthoDBiEOG091G0LT0.
PhylomeDBiQ9V359.

Enzyme and pathway databases

ReactomeiR-DME-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

ChiTaRSiVps28. fly.
GenomeRNAii47408.
PROiQ9V359.

Gene expression databases

BgeeiFBgn0021814.
ExpressionAtlasiQ9V359. baseline.
GenevisibleiQ9V359. DM.

Family and domain databases

InterProiIPR007143. VPS28.
IPR017899. VPS28_C.
IPR017898. VPS28_N.
[Graphical view]
PANTHERiPTHR12937. PTHR12937. 1 hit.
PfamiPF03997. VPS28. 1 hit.
[Graphical view]
PIRSFiPIRSF017535. VPS28. 1 hit.
PROSITEiPS51310. VPS28_C. 1 hit.
PS51313. VPS28_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVPS28_DROME
AccessioniPrimary (citable) accession number: Q9V359
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.