Q9V2Z7 (K6PF_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-specific phosphofructokinase
EC=2.7.1.146

Alternative name(s):
ADP-dependent phosphofructokinase
Short name=ADP-Pfk

Gene names

Name:	pfkC
Synonyms:	pfkA
Ordered Locus Names:	PF1784

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus furiosus

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate using ADP as the phosphate donor. As a phosphoryl group donor, ADP can be replaced by GDP, ATP, and GTP to a limited extent. HAMAP-Rule MF_00561
Catalytic activity	ADP + D-fructose 6-phosphate = AMP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00561
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Enzyme regulation	Inhibited by AMP and ATP. HAMAP-Rule MF_00561
Pathway	Carbohydrate degradation; glycolysis. HAMAP-Rule MF_00561
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm HAMAP-Rule MF_00561.
Sequence similarities	Belongs to the carbohydrate kinase PfkC family. Contains 1 ADPK (ADP-dependent kinase) domain.
Biophysicochemical properties	Kinetic parameters: The kinetic parameters were measured at 50 degrees Celsius. KM=2.3 mM for fructose 6-phosphate KM=0.11 mM for ADP Vmax=194 µmol/min/mg enzyme with fructose 6-phosphate as substrate Vmax=150 µmol/min/mg enzyme with ADP as substrate pH dependence: Optimum pH is 6.5.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ADP-specific phosphofructokinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphofructokinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	ADP-specific phosphofructokinase HAMAP-Rule MF_00561		PRO_0000184766
Regions
	Domain	1 – 452	452	ADPK
Sites
	Active site	436	1	Proton acceptor By similarity
	Metal binding	263	1	Magnesium By similarity
	Metal binding	293	1	Magnesium By similarity
	Metal binding	436	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V2Z7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 390F32584ECD0DC8	FASTA	454	52,337
	10 20 30 40 50 60 MIDEVRELGI YTAYNANVDA IVNLNAEIIQ RLIEEFGPDK IKRRLEEYPR EINEPLDFVA 70 80 90 100 110 120 RLVHALKTGK PMAVPLVNEE LHQWFDKTFK YDTERIGGQA GIIANILVGL KVKKVIAYTP 130 140 150 160 170 180 FLPKRLAELF KEGILYPVVE EDKLVLKPIQ SAYREGDPLK VNRIFEFRKG TRFKLGDEVI 190 200 210 220 230 240 EVPHSGRFIV SSRFESISRI ETKDELRKFL PEIGEMVDGA ILSGYQGIRL QYSDGKDANY 250 260 270 280 290 300 YLRRAKEDIR LLKKNKDIKI HVEFASIQDR RLRKKVVNNI FPMVDSVGMD EAEIAYILSV 310 320 330 340 350 360 LGYSDLADRI FMYNRIEDAI LGGMIILDEL NFEILQVHTI YYLMYITHRD NPLSEEELMR 370 380 390 400 410 420 SLDFGTILAA TRASLGDIND PRDVKVGMSV PYNERSEYIK LRFEEAKRKL RLKEYKVVIV 430 440 450 PTRLVPNPVS TVGLGDTIST GTFLSYLSLL RRHQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and biochemical characterization of the ADP-dependent phosphofructokinase from the hyperthermophilic archaeon Pyrococcus furiosus." Tuininga J.E., Verhees C.H., van der Oost J., Kengen S.W.M., Stams A.J.M., de Vos W.M. J. Biol. Chem. 274:21023-21028(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF127909 Genomic DNA. Translation: AAD48400.1. AE009950 Genomic DNA. Translation: AAL81908.1.
RefSeq	NP_579513.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q9V2Z7.
SMR	Q9V2Z7. Positions 8-453.
ModBase	Search...
Protein-protein interaction databases
STRING	186497.PF1784.
Proteomic databases
PRIDE	Q9V2Z7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL81908; AAL81908; PF1784.
GeneID	1469663.
KEGG	pfu:PF1784.
Phylogenomic databases
eggNOG	COG4809.
HOGENOM	HOG000254055.
KO	K00918.
OMA	HLEFASI.
ProtClustDB	PRK03979.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11808.
UniPathway	UPA00109.
Family and domain databases
HAMAP	MF_00561. ADP_PFKinase.
InterPro	IPR007666. ADP_PFK/GK. IPR015990. ADP_PFK/GK_arc. IPR011790. ADP_PFK_arc. [Graphical view]
PANTHER	PTHR21208. PTHR21208. 1 hit.
Pfam	PF04587. ADP_PFK_GK. 1 hit. [Graphical view]
PIRSF	PIRSF015883. ADP-Pfk_glckin. 1 hit.
TIGRFAMs	TIGR02045. P_fruct_ADP. 1 hit.
PROSITE	PS51255. ADPK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_PYRFU

Accession

Primary (citable) accession number: Q9V2Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program