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Protein

ADP-dependent glucokinase

Gene

glkA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. Can also use CDP as the phosphoryl group donor and D-glucosamine and D-1,5-anhydroglucitol as the phosphoryl group acceptor.2 Publications

Catalytic activityi

ADP + D-glucose = AMP + D-glucose 6-phosphate.2 Publications

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Kineticsi

  1. KM=0.64 mM for D-glucose (at 37 degrees Celsius)2 Publications
  2. KM=0.73 mM for D-glucose (at 50 degrees Celsius)2 Publications
  3. KM=0.54 mM for D-glucosamine (at 37 degrees Celsius)2 Publications
  4. KM=8.3 mM for D-1,5-anhydroglucitol (at 37 degrees Celsius)2 Publications
  5. KM=0.07 mM for ADP (at 37 degrees Celsius)2 Publications
  6. KM=0.033 mM for ADP (at 50 degrees Celsius)2 Publications
  7. KM=0.83 mM for CDP (at 37 degrees Celsius)2 Publications
  8. KM=0.041 mM for magnesium ions (at 37 degrees Celsius)2 Publications
  1. Vmax=249 µmol/min/mg enzyme toward glucose (at 50 degrees Celsius)2 Publications
  2. Vmax=194 µmol/min/mg enzyme toward ADP (at 50 degrees Celsius)2 Publications
  3. Vmax=160 µmol/min/mg enzyme toward glucose (at 37 degrees Celsius)2 Publications

pH dependencei

Optimum pH is about 7.5.2 Publications

Temperature dependencei

Optimum temperature is 105 degrees Celsius. Activity observed at 100 degrees Celsius is about 8 times that at 37 degrees Celsius. Thermostable up to 95 degrees Celsius. Retains full activity after heating at 90 degrees Celsius for 10 minutes and more than 95% of the full activity at 100 degrees Celsius for 10 minutes. Has a half-life of 220 minutes at 100 degrees Celsius. Inactive after heating at 110 degrees Celsius for 30 minutes.2 Publications

Pathwayi: glycolysis

This protein is involved in the pathway glycolysis, which is part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30Glucose1 Publication1
Binding sitei34Glucose1 Publication1
Binding sitei88Glucose1 Publication1
Binding sitei113Glucose1 Publication1
Binding sitei176Glucose1 Publication1
Binding sitei197Glucose1 Publication1
Metal bindingi266MagnesiumBy similarity1
Binding sitei292ADP1
Metal bindingi295MagnesiumBy similarity1
Binding sitei342ADP1
Binding sitei343ADP; via carbonyl oxygen1
Binding sitei429ADP; via carbonyl oxygen1
Active sitei440Proton acceptor1
Metal bindingi440MagnesiumBy similarity1
Binding sitei440ADP1
Binding sitei440Glucose1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi342 – 344ADP3
Nucleotide bindingi438 – 442ADP5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11804.
BRENDAi2.7.1.147. 5243.
SABIO-RKQ9V2Z6.
UniPathwayiUPA00109.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-dependent glucokinase (EC:2.7.1.147)
Short name:
ADP-GK
Short name:
ADPGK
Gene namesi
Name:glkA
Ordered Locus Names:PF0312
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001847722 – 455ADP-dependent glucokinaseAdd BLAST454

Proteomic databases

PRIDEiQ9V2Z6.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi186497.PF0312.

Structurei

Secondary structure

1455
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Helixi19 – 21Combined sources3
Beta strandi25 – 29Combined sources5
Beta strandi32 – 38Combined sources7
Helixi41 – 51Combined sources11
Helixi53 – 61Combined sources9
Beta strandi65 – 67Combined sources3
Helixi70 – 83Combined sources14
Beta strandi87 – 91Combined sources5
Helixi94 – 103Combined sources10
Beta strandi106 – 112Combined sources7
Helixi113 – 121Combined sources9
Turni122 – 125Combined sources4
Beta strandi129 – 131Combined sources3
Helixi138 – 141Combined sources4
Beta strandi146 – 155Combined sources10
Beta strandi158 – 162Combined sources5
Helixi164 – 166Combined sources3
Beta strandi175 – 181Combined sources7
Beta strandi196 – 201Combined sources6
Helixi206 – 208Combined sources3
Helixi213 – 215Combined sources3
Helixi219 – 222Combined sources4
Helixi223 – 225Combined sources3
Beta strandi227 – 231Combined sources5
Helixi234 – 236Combined sources3
Turni239 – 241Combined sources3
Helixi243 – 258Combined sources16
Beta strandi263 – 266Combined sources4
Helixi273 – 282Combined sources10
Helixi283 – 285Combined sources3
Beta strandi287 – 291Combined sources5
Helixi293 – 302Combined sources10
Helixi306 – 311Combined sources6
Beta strandi314 – 317Combined sources4
Helixi320 – 334Combined sources15
Beta strandi337 – 342Combined sources6
Beta strandi344 – 353Combined sources10
Helixi356 – 375Combined sources20
Helixi381 – 386Combined sources6
Helixi387 – 389Combined sources3
Helixi396 – 407Combined sources12
Beta strandi413 – 416Combined sources4
Beta strandi419 – 425Combined sources7
Helixi438 – 452Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UA4X-ray1.90A1-455[»]
ProteinModelPortaliQ9V2Z6.
SMRiQ9V2Z6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9V2Z6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 455ADPKAdd BLAST454

Sequence similaritiesi

Belongs to the ADP-dependent glucokinase family.Curated

Phylogenomic databases

eggNOGiarCOG03371. Archaea.
COG4809. LUCA.
HOGENOMiHOG000254055.
KOiK00918.
OMAiLAYNTNI.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00809. ADP_glucokinase. 1 hit.
InterProiIPR031299. ADP_GK.
IPR007666. ADP_PFK/GK.
IPR015990. ADP_PFK/GK_arc.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
PIRSFiPIRSF015883. ADP-Pfk_glckin. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9V2Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTWEELYKN AIEKAIKSVP KVKGVLLGYN TNIDAIKYLD SKDLEERIIK
60 70 80 90 100
AGKEEVIKYS EELPDKINTV SQLLGSILWS IRRGKAAELF VESCPVRFYM
110 120 130 140 150
KRWGWNELRM GGQAGIMANL LGGVYGVPVI VHVPQLSRLQ ANLFLDGPIY
160 170 180 190 200
VPTLENGEVK LIHPKEFSGD EENCIHYIYE FPRGFRVFEF EAPRENRFIG
210 220 230 240 250
SADDYNTTLF IREEFRESFS EVIKNVQLAI LSGLQALTKE NYKEPFEIVK
260 270 280 290 300
SNLEVLNERE IPVHLEFAFT PDEKVREEIL NVLGMFYSVG LNEVELASIM
310 320 330 340 350
EILGEKKLAK ELLAHDPVDP IAVTEAMLKL AKKTGVKRIH FHTYGYYLAL
360 370 380 390 400
TEYKGEHVRD ALLFAALAAA AKAMKGNITS LEEIREATSV PVNEKATQVE
410 420 430 440 450
EKLRAEYGIK EGIGEVEGYQ IAFIPTKIVA KPKSTVGIGD TISSSAFIGE

FSFTL
Length:455
Mass (Da):51,266
Last modified:January 23, 2007 - v3
Checksum:i8262ACBF19BED113
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF127910 Genomic DNA. Translation: AAD48401.1.
E14588 Unassigned DNA. No translation available.
AE009950 Genomic DNA. Translation: AAL80436.1.
PIRiJC7550.
RefSeqiWP_011011427.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80436; AAL80436; PF0312.
GeneIDi1468147.
KEGGipfu:PF0312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF127910 Genomic DNA. Translation: AAD48401.1.
E14588 Unassigned DNA. No translation available.
AE009950 Genomic DNA. Translation: AAL80436.1.
PIRiJC7550.
RefSeqiWP_011011427.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UA4X-ray1.90A1-455[»]
ProteinModelPortaliQ9V2Z6.
SMRiQ9V2Z6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0312.

Proteomic databases

PRIDEiQ9V2Z6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80436; AAL80436; PF0312.
GeneIDi1468147.
KEGGipfu:PF0312.

Phylogenomic databases

eggNOGiarCOG03371. Archaea.
COG4809. LUCA.
HOGENOMiHOG000254055.
KOiK00918.
OMAiLAYNTNI.

Enzyme and pathway databases

UniPathwayiUPA00109.
BioCyciMetaCyc:MONOMER-11804.
BRENDAi2.7.1.147. 5243.
SABIO-RKQ9V2Z6.

Miscellaneous databases

EvolutionaryTraceiQ9V2Z6.
PROiQ9V2Z6.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00809. ADP_glucokinase. 1 hit.
InterProiIPR031299. ADP_GK.
IPR007666. ADP_PFK/GK.
IPR015990. ADP_PFK/GK_arc.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
PIRSFiPIRSF015883. ADP-Pfk_glckin. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLKA_PYRFU
AccessioniPrimary (citable) accession number: Q9V2Z6
Secondary accession number(s): Q7LX13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.