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Protein

Proteasome subunit alpha 2

Gene

psmA2

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The H.volcanii alpha1-beta-alpha2 proteasome is able to cleave oligopeptides after Tyr and thus displays chymotrypsin-like activity.UniRule annotation2 Publications

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation2 Publications

Enzyme regulationi

The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

GO - Molecular functioni

  • endopeptidase activity Source: UniProtKB
  • threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha 2UniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome alpha subunit 2UniRule annotation
Proteasome core protein PsmA 2UniRule annotation
Gene namesi
Name:psmA2UniRule annotation
Synonyms:psmC
Ordered Locus Names:HVO_2923
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloferax
Proteomesi
  • UP000008243 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • proteasome core complex, alpha-subunit complex Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Proteasome

Pathology & Biotechi

Disruption phenotypei

Strains lacking psmA2 gene alone display relatively normal growth rate and overall cell yield. However, depletion of psmA1 and psmA2 together renders the cells inviable.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241721 – 249Proteasome subunit alpha 2Add BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Keywords - PTMi

Acetylation

PTM databases

iPTMnetiQ9V2V5

Expressioni

Inductioni

Up-regulated at mRNA and protein levels during transition from exponential to stationary phase.1 Publication

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. H.volcanii produces at least 2 types of 20S proteasomes: an alpha1-beta proteasome and a proteasome containing all three subunits (alpha1, alpha2, and beta) that appears to be asymmetrical with homo-oligomeric alpha1 and alpha2 rings positioned on separate ends. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped at one or both ends by the proteasome regulatory ATPase, PAN.2 Publications

Protein-protein interaction databases

STRINGi309800.HVO_2923

Structurei

3D structure databases

ProteinModelPortaliQ9V2V5
SMRiQ9V2V5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00971 Archaea
COG0638 LUCA
HOGENOMiHOG000091085
KOiK03432
OMAiFAWKASA
OrthoDBiPOG093Z086S

Family and domain databases

CDDicd03756 proteasome_alpha_archeal, 1 hit
Gene3Di3.60.20.10, 1 hit
HAMAPiMF_00289_A Proteasome_A_A, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR019982 Proteasome_asu_arc
IPR000426 Proteasome_asu_N
IPR001353 Proteasome_sua/b
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9V2V5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRNDKQAYD RGTSLFSPDG RIYQVEYARE AVKRGAPVLG VRTADGVVLA
60 70 80 90 100
ALRSTPSELM EAESIEKLHK LDDALGAATA GHVADARKLV DFARTTAQRE
110 120 130 140 150
HLRYGEPIGV ETLTKTITDN IQESTQSGGT RPYGASLLIG GVENGSGRLF
160 170 180 190 200
ATDPSGTPQE WKAVAIGGHR EDVQAALEDG YAEDLSLEDG LALAVEALVA
210 220 230 240
ADEEIESDEL NLVTVSEAGY EIVDEETIAE LFADATADDE SDETDEREE
Length:249
Mass (Da):26,727
Last modified:May 1, 2000 - v1
Checksum:i7205A1A2B93E344E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126261 Genomic DNA Translation: AAD53405.1
CP001956 Genomic DNA Translation: ADE02793.1
PIRiT48679
RefSeqiWP_004044756.1, NZ_AOHU01000104.1

Genome annotation databases

EnsemblBacteriaiADE02793; ADE02793; HVO_2923
GeneIDi8926453
KEGGihvo:HVO_2923

Similar proteinsi

Entry informationi

Entry nameiPSA2_HALVD
AccessioniPrimary (citable) accession number: Q9V2V5
Secondary accession number(s): D4GXU2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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