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Q9V2V5

- PSA2_HALVD

UniProt

Q9V2V5 - PSA2_HALVD

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Protein

Proteasome subunit alpha 2

Gene

psmA2

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The H.volcanii alpha1-beta-alpha2 proteasome is able to cleave oligopeptides after Tyr and thus displays chymotrypsin-like activity.2 PublicationsUniRule annotation

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.2 PublicationsUniRule annotation

Enzyme regulationi

The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

GO - Molecular functioni

  1. endopeptidase activity Source: UniProtKB
  2. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteasomal protein catabolic process Source: UniProtKB
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciHVOL309800:GCOK-2929-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha 2UniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome alpha subunit 2UniRule annotation
Proteasome core protein PsmA 2UniRule annotation
Gene namesi
Name:psmA2UniRule annotation
Synonyms:psmC
Ordered Locus Names:HVO_2923
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
ProteomesiUP000008243: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. proteasome core complex, alpha-subunit complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

Pathology & Biotechi

Disruption phenotypei

Strains lacking psmA2 gene alone display relatively normal growth rate and overall cell yield. However, depletion of psmA1 and psmA2 together renders the cells inviable.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Proteasome subunit alpha 2PRO_0000124172Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Expressioni

Inductioni

Up-regulated at mRNA and protein levels during transition from exponential to stationary phase.1 Publication

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. H.volcanii produces at least 2 types of 20S proteasomes: an alpha1-beta proteasome and a proteasome containing all three subunits (alpha1, alpha2, and beta) that appears to be asymmetrical with homo-oligomeric alpha1 and alpha2 rings positioned on separate ends. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped at one or both ends by the proteasome regulatory ATPase, PAN.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ9V2V5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000091085.
KOiK03432.
OMAiRNDKQAY.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00289_A. Proteasome_A_A.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019982. Proteasome_asu_arc.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9V2V5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRNDKQAYD RGTSLFSPDG RIYQVEYARE AVKRGAPVLG VRTADGVVLA
60 70 80 90 100
ALRSTPSELM EAESIEKLHK LDDALGAATA GHVADARKLV DFARTTAQRE
110 120 130 140 150
HLRYGEPIGV ETLTKTITDN IQESTQSGGT RPYGASLLIG GVENGSGRLF
160 170 180 190 200
ATDPSGTPQE WKAVAIGGHR EDVQAALEDG YAEDLSLEDG LALAVEALVA
210 220 230 240
ADEEIESDEL NLVTVSEAGY EIVDEETIAE LFADATADDE SDETDEREE
Length:249
Mass (Da):26,727
Last modified:May 1, 2000 - v1
Checksum:i7205A1A2B93E344E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126261 Genomic DNA. Translation: AAD53405.1.
CP001956 Genomic DNA. Translation: ADE02793.1.
PIRiT48679.
RefSeqiWP_004044756.1. NZ_AOHU01000104.1.
YP_003536933.1. NC_013967.1.

Genome annotation databases

EnsemblBacteriaiADE02793; ADE02793; HVO_2923.
GeneIDi8926453.
KEGGihvo:HVO_2923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126261 Genomic DNA. Translation: AAD53405.1 .
CP001956 Genomic DNA. Translation: ADE02793.1 .
PIRi T48679.
RefSeqi WP_004044756.1. NZ_AOHU01000104.1.
YP_003536933.1. NC_013967.1.

3D structure databases

ProteinModelPortali Q9V2V5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADE02793 ; ADE02793 ; HVO_2923 .
GeneIDi 8926453.
KEGGi hvo:HVO_2923.

Phylogenomic databases

HOGENOMi HOG000091085.
KOi K03432.
OMAi RNDKQAY.

Enzyme and pathway databases

BioCyci HVOL309800:GCOK-2929-MONOMER.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_00289_A. Proteasome_A_A.
InterProi IPR029055. Ntn_hydrolases_N.
IPR019982. Proteasome_asu_arc.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view ]
SMARTi SM00948. Proteasome_A_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Halophilic 20S proteasomes of the archaeon Haloferax volcanii: purification, characterization, and gene sequence analysis."
    Wilson H.L., Aldrich H.C., Maupin-Furlow J.
    J. Bacteriol. 181:5814-5824(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-31; 34-43 AND 89-98, BLOCKAGE OF N-TERMINUS, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
  3. "Subunit topology of two 20S proteasomes from Haloferax volcanii."
    Kaczowka S.J., Maupin-Furlow J.A.
    J. Bacteriol. 185:165-174(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, FUNCTION, CATALYTIC ACTIVITY.
  4. "Differential regulation of the PanA and PanB proteasome-activating nucleotidase and 20S proteasomal proteins of the haloarchaeon Haloferax volcanii."
    Reuter C.J., Kaczowka S.J., Maupin-Furlow J.A.
    J. Bacteriol. 186:7763-7772(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Posttranslational modification of the 20S proteasomal proteins of the archaeon Haloferax volcanii."
    Humbard M.A., Stevens S.M. Jr., Maupin-Furlow J.A.
    J. Bacteriol. 188:7521-7530(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT MET-1.
  6. "Proteasomal components required for cell growth and stress responses in the haloarchaeon Haloferax volcanii."
    Zhou G., Kowalczyk D., Humbard M.A., Rohatgi S., Maupin-Furlow J.A.
    J. Bacteriol. 190:8096-8105(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPSA2_HALVD
AccessioniPrimary (citable) accession number: Q9V2V5
Secondary accession number(s): D4GXU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3