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Q9V2V5

- PSA2_HALVD

UniProt

Q9V2V5 - PSA2_HALVD

Protein

Proteasome subunit alpha 2

Gene

psmA2

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The H.volcanii alpha1-beta-alpha2 proteasome is able to cleave oligopeptides after Tyr and thus displays chymotrypsin-like activity.2 PublicationsUniRule annotation

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.2 PublicationsUniRule annotation

    Enzyme regulationi

    The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

    GO - Molecular functioni

    1. endopeptidase activity Source: UniProtKB
    2. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteasomal protein catabolic process Source: UniProtKB
    2. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    BioCyciHVOL309800:GCOK-2929-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit alpha 2UniRule annotation (EC:3.4.25.1UniRule annotation)
    Alternative name(s):
    20S proteasome alpha subunit 2UniRule annotation
    Proteasome core protein PsmA 2UniRule annotation
    Gene namesi
    Name:psmA2UniRule annotation
    Synonyms:psmC
    Ordered Locus Names:HVO_2923
    OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
    Taxonomic identifieri309800 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
    ProteomesiUP000008243: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. proteasome core complex, alpha-subunit complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Proteasome

    Pathology & Biotechi

    Disruption phenotypei

    Strains lacking psmA2 gene alone display relatively normal growth rate and overall cell yield. However, depletion of psmA1 and psmA2 together renders the cells inviable.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 249249Proteasome subunit alpha 2PRO_0000124172Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Expressioni

    Inductioni

    Up-regulated at mRNA and protein levels during transition from exponential to stationary phase.1 Publication

    Interactioni

    Subunit structurei

    The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. H.volcanii produces at least 2 types of 20S proteasomes: an alpha1-beta proteasome and a proteasome containing all three subunits (alpha1, alpha2, and beta) that appears to be asymmetrical with homo-oligomeric alpha1 and alpha2 rings positioned on separate ends. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped at one or both ends by the proteasome regulatory ATPase, PAN.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ9V2V5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1A family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000091085.
    KOiK03432.
    OMAiRNDKQAY.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_00289_A. Proteasome_A_A.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR019982. Proteasome_asu_arc.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00948. Proteasome_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9V2V5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRNDKQAYD RGTSLFSPDG RIYQVEYARE AVKRGAPVLG VRTADGVVLA    50
    ALRSTPSELM EAESIEKLHK LDDALGAATA GHVADARKLV DFARTTAQRE 100
    HLRYGEPIGV ETLTKTITDN IQESTQSGGT RPYGASLLIG GVENGSGRLF 150
    ATDPSGTPQE WKAVAIGGHR EDVQAALEDG YAEDLSLEDG LALAVEALVA 200
    ADEEIESDEL NLVTVSEAGY EIVDEETIAE LFADATADDE SDETDEREE 249
    Length:249
    Mass (Da):26,727
    Last modified:May 1, 2000 - v1
    Checksum:i7205A1A2B93E344E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF126261 Genomic DNA. Translation: AAD53405.1.
    CP001956 Genomic DNA. Translation: ADE02793.1.
    PIRiT48679.
    RefSeqiWP_004044756.1. NZ_AOHU01000104.1.
    YP_003536933.1. NC_013967.1.

    Genome annotation databases

    EnsemblBacteriaiADE02793; ADE02793; HVO_2923.
    GeneIDi8926453.
    KEGGihvo:HVO_2923.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF126261 Genomic DNA. Translation: AAD53405.1 .
    CP001956 Genomic DNA. Translation: ADE02793.1 .
    PIRi T48679.
    RefSeqi WP_004044756.1. NZ_AOHU01000104.1.
    YP_003536933.1. NC_013967.1.

    3D structure databases

    ProteinModelPortali Q9V2V5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADE02793 ; ADE02793 ; HVO_2923 .
    GeneIDi 8926453.
    KEGGi hvo:HVO_2923.

    Phylogenomic databases

    HOGENOMi HOG000091085.
    KOi K03432.
    OMAi RNDKQAY.

    Enzyme and pathway databases

    BioCyci HVOL309800:GCOK-2929-MONOMER.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_00289_A. Proteasome_A_A.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR019982. Proteasome_asu_arc.
    IPR000426. Proteasome_asu_N.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    PF10584. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00948. Proteasome_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00388. PROTEASOME_ALPHA_1. 1 hit.
    PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Halophilic 20S proteasomes of the archaeon Haloferax volcanii: purification, characterization, and gene sequence analysis."
      Wilson H.L., Aldrich H.C., Maupin-Furlow J.
      J. Bacteriol. 181:5814-5824(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-31; 34-43 AND 89-98, BLOCKAGE OF N-TERMINUS, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
    3. "Subunit topology of two 20S proteasomes from Haloferax volcanii."
      Kaczowka S.J., Maupin-Furlow J.A.
      J. Bacteriol. 185:165-174(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, FUNCTION, CATALYTIC ACTIVITY.
    4. "Differential regulation of the PanA and PanB proteasome-activating nucleotidase and 20S proteasomal proteins of the haloarchaeon Haloferax volcanii."
      Reuter C.J., Kaczowka S.J., Maupin-Furlow J.A.
      J. Bacteriol. 186:7763-7772(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    5. "Posttranslational modification of the 20S proteasomal proteins of the archaeon Haloferax volcanii."
      Humbard M.A., Stevens S.M. Jr., Maupin-Furlow J.A.
      J. Bacteriol. 188:7521-7530(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION AT MET-1.
    6. "Proteasomal components required for cell growth and stress responses in the haloarchaeon Haloferax volcanii."
      Zhou G., Kowalczyk D., Humbard M.A., Rohatgi S., Maupin-Furlow J.A.
      J. Bacteriol. 190:8096-8105(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPSA2_HALVD
    AccessioniPrimary (citable) accession number: Q9V2V5
    Secondary accession number(s): D4GXU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 8, 2000
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3