Reviewed, UniProtKB/Swiss-Prot Q9V2R0 (NADB_PYRAB)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	PYRAB00150
ORF Names:	PAB2343

Organism

Pyrococcus abyssi [Complete proteome] [HAMAP]

Taxonomic identifier

29292 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + H(2)O + O(2) = oxaloacetate + NH(3) + H(2)O(2).
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	L-aspartate oxidase		PRO_0000184408
Regions
	Nucleotide binding	7 – 21	15	FAD Potential
Sites
	Active site	213	1	By similarity
	Active site	230	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V2R0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E08588BF1BD6AABC	FASTA	464	50,939
	10 20 30 40 50 60 MIDMEVGIVG GGLAGLVAAI SLVEKGVDVS IIGPKSKDSN SYLAQAGIAF PLVEGDSIRI 70 80 90 100 110 120 HVLDTIRAGK YINDEEVVWN VISKSTEAYS FLVSHGVTFT GNELEGGHSH PRVFTIKSET 130 140 150 160 170 180 GKHVIPILEK HARELGVNFV RGFVEEIGIK NGKLAGVFLN GELLKFDAVV VATGGFSGLY 190 200 210 220 230 240 RFTAGVKENI GLLIGDLALK GVPLRDMEFV QFHPTGFIGR RTYLITEAVR GAGAKLVTGD 250 260 270 280 290 300 GERFVNELET RDVVARAIYL KMLEGKGVFL DARGIEDFKD RFPYVYSVLK KEGIDPGKDL 310 320 330 340 350 360 IPVTPVAHYT MGGISVDIFY RTRIRGLYAI GEAASNGFHG ANRLASNSLL ECVVSGLEVA 370 380 390 400 410 420 RTILREEPKR GANDAPYNFD ELGDVDSIRE IMWNHAGIVR DKSSLLEGLK KLEGVEADQR 430 440 450 460 LKVVAKAVLT LALEREESRG SHYRRDFPFM RKEFERPSFF HLNV

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ248283 Genomic DNA. Translation: CAB48938.1.
PIR	C75186.
RefSeq	NP_125706.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Genome annotation databases
GeneID	1495699.
GenomeReviews	Gene locus PYRAB00150 in contig AL096836_GR.
KEGG	pab:PAB2343.
NMPDR	fig|272844.1.peg.18.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9V2R0.
Enzyme and pathway databases
BioCyc	PABY272844:PAB2343-MON.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004112. Fum_Rdtase/Succ_DHase_flav_C. IPR005288. NadB. IPR001100. Pyr_nuc-diS_OxRdtase. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00411. PNDRDTASEI.
TIGRFAMs	TIGR00551. nadB. 1 hit.
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

NADB_PYRAB

Accession

Primary (citable) accession number: Q9V2R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	May 1, 2000
Last modified:	November 4, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)