Replication factor C small subunit - Pyrococcus abyssi (strain GE5 / Orsay)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Replication factor C small subunit
Short name=RFC small subunit

Alternative name(s):
Clamp loader small subunit
PabRFC small subunit

Cleaved into the following 2 chains:

Pab RFC-1 intein
Pab RFC-2 intein

Gene names

Name:	rfcS
Ordered Locus Names:	PYRAB01100
ORF Names:	PAB0068

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	1437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-independent ATPase activity. Ref.2
Subunit structure	Heterohexamer composed of four small subunits (rfcS) and two large subunits (rfcL) Probable. Ref.2
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP MF_01509
Miscellaneous	The intein interrupts the potential ATP-binding site. HAMAP MF_01509
Sequence similarities	Belongs to the activator 1 small subunits family. RfcS subfamily. Contains 2 DOD-type homing endonuclease domains.

Ontologies

Keywords
Biological process	DNA replication
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	DNA replication factor C complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA clamp loader activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 61

61

Replication factor C small subunit, 1st part Potential

PRO_0000030371

Chain

62 – 560

499

Pab RFC-1 intein Potential

PRO_0000030372

Chain

561 – 647

87

Replication factor C small subunit, 2nd part Potential

PRO_0000030373

Chain

648 – 1255

608

Pab RFC-2 intein Potential

PRO_0000030374

Chain

1256 – 1437

182

Replication factor C small subunit, 3rd part Potential

PRO_0000030375

Regions

Domain

185 – 301

117

DOD-type homing endonuclease 1

Domain

940 – 1098

159

DOD-type homing endonuclease 2

Sequences

Sequence

Length

Mass (Da)

Tools

Q9V2G4 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 9725AC4F4240C985
Show »

FASTA

1,437

165,246

        10         20         30         40         50         60 
MRDMEEVREV KVLEKPWVEK YRPQKLEEIV GQEHIVKRLK HYVKTGSMPH LLFAGPPGVG 

        70         80         90        100        110        120 
KCLTGDAKVI ANGELTTIGE LVERISNGKL GPTPVRGLTV LGIDEDGKLV ELPVEYVYKD 

       130        140        150        160        170        180 
KTSELVKIRT RLGRELKVTP YHPLLVNRRN GKIEWVKAEE LKPGDRLAIP SFLPAMLNDN 

       190        200        210        220        230        240 
PLAEWLGYFF GNGYTDSEER VVFESKSKEL RKRFMELTRK LFQDAEIKED SGKVYVSSSE 

       250        260        270        280        290        300 
VKRLVKSLNK DSIPEQAWKG LRSFLRAYFD CNAEIKDKII VSTAGKEIAE QISYALAGLG 

       310        320        330        340        350        360 
IVAEVDDKGS VIISDPENVS RFLDEIGFSV EEKKEEAKAL IKKSTLNLGI YVDKELISYV 

       370        380        390        400        410        420 
REKLKLSFYE NETMWSPEKA REIAWKLMKE IYYRLDELER FKKALSKSVI IDWSEVEKKK 

       430        440        450        460        470        480 
EEISEKTGIS VNEILEYAKG KRKPSLEEYV KIAKALGVEL KETLEAIFTF GKKYLGYVIS 

       490        500        510        520        530        540 
DEIETLEEVR KEELKRLKEL LNDEKLKKGV AYLIFLAQNE LLWDEIIEVE KLKGDFVIYD 

       550        560        570        580        590        600 
LHVPKYHNFI GGNLPTVLHN TTAALALARE LFGENWRHNF LELNASDERG INVIREKVKE 

       610        620        630        640        650        660 
FARTKPIGGA SFKIIFLDEA DALTQDAQQA LRRTMEMFSS NVRFILSCVT GDTKVYTPDE 

       670        680        690        700        710        720 
REVKIRDFMN YFENGLIKEV SNRIGRDTVI AAVSFNSRIV GHPVYRLTLE SGRIIEATGD 

       730        740        750        760        770        780 
HMFLTPEGWK QTYDIKEGSE VLVKPTLEGT PYEPDPRVII DIKEFYNFLE KIEREHNLKP 

       790        800        810        820        830        840 
LKEAKTFREL ITKDKEKILR RALELRAEIE NGLTKREAEI LELISADTWI PRAELEKKAR 

       850        860        870        880        890        900 
ISRTRLNQIL QRLEKKGYIE RRIEGRKQFV RKIRNGKILR NAMDIKRILE EEFGIKISYT 

       910        920        930        940        950        960 
TVKKLLSGNV DGMAYRILKE VKEKWLVRYD DEKAGILARV VGFILGDGHL ARNGRIWFNS 

       970        980        990       1000       1010       1020 
SKEELEMLAN DLRKLGLKPS EIIERDSSSE IQGRKVKGRI YMLYVDNAAF HALLRFWKVE 

      1030       1040       1050       1060       1070       1080 
VGNKTKKGYT VPEWIKKGNL FVKREFLRGL FGADGTKPCG KRYNFNGIKL EIRAKKESLE 

      1090       1100       1110       1120       1130       1140 
RTVEFLNDVA DLLREFDVDS KITVSPTKEG FIIRLIVTPN DANYLNFLTR VGYAYAKDTY 

      1150       1160       1170       1180       1190       1200 
ARLVGEYIRI KLAYKNIILP GIAEKAIELA TVTNSTYAAK VLGVSRDFVV NRLKGTQIGI 

      1210       1220       1230       1240       1250       1260 
TRDFMTFEEF MKERVLNGYV IEKVIKKEKL GYLDVYDVTC ARDHSFISNG LVSHNCNYSS 

      1270       1280       1290       1300       1310       1320 
KIIEPIQSRC AIFRFRPLND EDIAKRLRYI AENEGLELTE EGLQAILYIA EGDMRRAINI 

      1330       1340       1350       1360       1370       1380 
LQAAAALDRK ITDENVFLVA SRARPEDIRE MMLLALKGNF LKAREKLREI LLKQGLSGED 

      1390       1400       1410       1420       1430 
VLVQMHREVF NLPIDEPMKV YLADKIGEYN FRLVEGANEM IQLEALLAQF TLIGKKK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.
[2]	"Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain." Henneke G., Gueguen Y., Flament D., Azam P., Querellou J., Dietrich J., Huebscher U., Raffin J.-P. J. Mol. Biol. 323:795-810(2002) [PubMed: 12417194] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, SUBUNIT. Strain: GE5 / Orsay.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248283 Genomic DNA. Translation: CAB49034.1.
PIR	C75198.
RefSeq	NP_125803.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V2G4.
SMR	Q9V2G4. Positions 5-61, 524-668, 1246-1436.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002193; EBPYRP00000002124; EBPYRG00000002193.
GeneID	1494997.
GenomeReviews	Gene locus PYRAB01100 in contig AL096836_GR.
KEGG	pab:PAB0068.
NMPDR	fig\|272844.1.peg.121.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022153.
HOGENOM	HBG541019.
OMA	YDDEKAG.
PhylomeDB	Q9V2G4.
ProtClustDB	PRK04132.
Enzyme and pathway databases
BioCyc	PABY272844:PAB0068-MONOMER.
Family and domain databases
HAMAP	MF_01509. RfcS. Divergent sequence. [Tree]
InterPro	IPR003959. ATPase_AAA_core. IPR008824. DNA_helicase_Holl-junc_RuvB_N. IPR008921. DNA_pol3_clamp-load_cplx_C. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR001387. HTH_3. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR010982. Lambda_DNA-bd. IPR013748. Rep_factor-C. IPR002831. Tscrpt_reg_TrmB_N. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.260.40. G3DSA:1.10.260.40. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K04801.
Pfam	PF00004. AAA. 1 hit. PF01381. HTH_3. 1 hit. PF08542. Rep_fac_C. 1 hit. PF05496. RuvB_N. 1 hit. PF01978. TrmB. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 2 hits. SM00306. HintN. 2 hits. SM00530. HTH_XRE. 1 hit. [Graphical view]
SUPFAM	SSF47413. Lambda_like_DNA. 1 hit. SSF48019. Pol_clamp_load_C. 1 hit.
TIGRFAMs	TIGR01443. Intein_Cterm. 2 hits. TIGR01445. Intein_Nterm. 2 hits.
PROSITE	PS50818. INTEIN_C_TER. 2 hits. PS50819. INTEIN_ENDONUCLEASE. 2 hits. PS50817. INTEIN_N_TER. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFCS_PYRAB

Accession

Primary (citable) accession number: Q9V2G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Cleaved into the following 2 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents