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Q9V2G4

- RFCS_PYRAB

UniProt

Q9V2G4 - RFCS_PYRAB

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Protein
Replication factor C small subunit
Gene
rfcS, PYRAB01100, PAB0068
Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-independent ATPase activity.1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. endonuclease activity Source: InterPro
  3. four-way junction helicase activity Source: InterPro
  4. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: InterPro
  2. DNA repair Source: InterPro
  3. DNA replication Source: UniProtKB-KW
  4. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C small subunit
Short name:
RFC small subunit
Alternative name(s):
Clamp loader small subunit
PabRFC small subunit
Cleaved into the following 2 chains:
Gene namesi
Name:rfcS
Ordered Locus Names:PYRAB01100
ORF Names:PAB0068
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000810: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6161Replication factor C small subunit, 1st part Reviewed prediction
PRO_0000030371Add
BLAST
Chaini62 – 560499Pab RFC-1 intein Reviewed prediction
PRO_0000030372Add
BLAST
Chaini561 – 64787Replication factor C small subunit, 2nd part Reviewed prediction
PRO_0000030373Add
BLAST
Chaini648 – 1255608Pab RFC-2 intein Reviewed prediction
PRO_0000030374Add
BLAST
Chaini1256 – 1437182Replication factor C small subunit, 3rd part Reviewed prediction
PRO_0000030375Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Heterohexamer composed of four small subunits (RfcS) and two large subunits (RfcL) Inferred.1 Publication

Protein-protein interaction databases

IntActiQ9V2G4. 1 interaction.
MINTiMINT-7906090.
STRINGi272844.PAB0068.

Structurei

3D structure databases

ProteinModelPortaliQ9V2G4.
SMRiQ9V2G4. Positions 5-61, 524-668, 1246-1436.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini185 – 301117DOD-type homing endonuclease 1
Add
BLAST
Domaini940 – 1098159DOD-type homing endonuclease 2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000154102.
KOiK04801.
OMAiTNDENEM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.260.40. 1 hit.
2.170.16.10. 6 hits.
3.10.28.10. 2 hits.
3.40.50.300. 3 hits.
InterProiIPR003959. ATPase_AAA_core.
IPR001387. Cro/C1-type_HTH.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 2 hits.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 5 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEiPS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9V2G4-1 [UniParc]FASTAAdd to Basket

« Hide

MRDMEEVREV KVLEKPWVEK YRPQKLEEIV GQEHIVKRLK HYVKTGSMPH     50
LLFAGPPGVG KCLTGDAKVI ANGELTTIGE LVERISNGKL GPTPVRGLTV 100
LGIDEDGKLV ELPVEYVYKD KTSELVKIRT RLGRELKVTP YHPLLVNRRN 150
GKIEWVKAEE LKPGDRLAIP SFLPAMLNDN PLAEWLGYFF GNGYTDSEER 200
VVFESKSKEL RKRFMELTRK LFQDAEIKED SGKVYVSSSE VKRLVKSLNK 250
DSIPEQAWKG LRSFLRAYFD CNAEIKDKII VSTAGKEIAE QISYALAGLG 300
IVAEVDDKGS VIISDPENVS RFLDEIGFSV EEKKEEAKAL IKKSTLNLGI 350
YVDKELISYV REKLKLSFYE NETMWSPEKA REIAWKLMKE IYYRLDELER 400
FKKALSKSVI IDWSEVEKKK EEISEKTGIS VNEILEYAKG KRKPSLEEYV 450
KIAKALGVEL KETLEAIFTF GKKYLGYVIS DEIETLEEVR KEELKRLKEL 500
LNDEKLKKGV AYLIFLAQNE LLWDEIIEVE KLKGDFVIYD LHVPKYHNFI 550
GGNLPTVLHN TTAALALARE LFGENWRHNF LELNASDERG INVIREKVKE 600
FARTKPIGGA SFKIIFLDEA DALTQDAQQA LRRTMEMFSS NVRFILSCVT 650
GDTKVYTPDE REVKIRDFMN YFENGLIKEV SNRIGRDTVI AAVSFNSRIV 700
GHPVYRLTLE SGRIIEATGD HMFLTPEGWK QTYDIKEGSE VLVKPTLEGT 750
PYEPDPRVII DIKEFYNFLE KIEREHNLKP LKEAKTFREL ITKDKEKILR 800
RALELRAEIE NGLTKREAEI LELISADTWI PRAELEKKAR ISRTRLNQIL 850
QRLEKKGYIE RRIEGRKQFV RKIRNGKILR NAMDIKRILE EEFGIKISYT 900
TVKKLLSGNV DGMAYRILKE VKEKWLVRYD DEKAGILARV VGFILGDGHL 950
ARNGRIWFNS SKEELEMLAN DLRKLGLKPS EIIERDSSSE IQGRKVKGRI 1000
YMLYVDNAAF HALLRFWKVE VGNKTKKGYT VPEWIKKGNL FVKREFLRGL 1050
FGADGTKPCG KRYNFNGIKL EIRAKKESLE RTVEFLNDVA DLLREFDVDS 1100
KITVSPTKEG FIIRLIVTPN DANYLNFLTR VGYAYAKDTY ARLVGEYIRI 1150
KLAYKNIILP GIAEKAIELA TVTNSTYAAK VLGVSRDFVV NRLKGTQIGI 1200
TRDFMTFEEF MKERVLNGYV IEKVIKKEKL GYLDVYDVTC ARDHSFISNG 1250
LVSHNCNYSS KIIEPIQSRC AIFRFRPLND EDIAKRLRYI AENEGLELTE 1300
EGLQAILYIA EGDMRRAINI LQAAAALDRK ITDENVFLVA SRARPEDIRE 1350
MMLLALKGNF LKAREKLREI LLKQGLSGED VLVQMHREVF NLPIDEPMKV 1400
YLADKIGEYN FRLVEGANEM IQLEALLAQF TLIGKKK 1437
Length:1,437
Mass (Da):165,246
Last modified:May 1, 2000 - v1
Checksum:i9725AC4F4240C985
GO

Sequence cautioni

The sequence CCE69486.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ248283 Genomic DNA. Translation: CAB49034.1.
HE613800 Genomic DNA. Translation: CCE69486.1. Different initiation.
PIRiC75198.
RefSeqiNP_125803.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB49034; CAB49034; PAB0068.
GeneIDi1494997.
KEGGipab:PAB0068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ248283 Genomic DNA. Translation: CAB49034.1 .
HE613800 Genomic DNA. Translation: CCE69486.1 . Different initiation.
PIRi C75198.
RefSeqi NP_125803.1. NC_000868.1.

3D structure databases

ProteinModelPortali Q9V2G4.
SMRi Q9V2G4. Positions 5-61, 524-668, 1246-1436.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9V2G4. 1 interaction.
MINTi MINT-7906090.
STRINGi 272844.PAB0068.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB49034 ; CAB49034 ; PAB0068 .
GeneIDi 1494997.
KEGGi pab:PAB0068.

Phylogenomic databases

eggNOGi COG1372.
HOGENOMi HOG000154102.
KOi K04801.
OMAi TNDENEM.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
1.10.260.40. 1 hit.
2.170.16.10. 6 hits.
3.10.28.10. 2 hits.
3.40.50.300. 3 hits.
InterProi IPR003959. ATPase_AAA_core.
IPR001387. Cro/C1-type_HTH.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
PF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 2 hits.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00530. HTH_XRE. 1 hit.
[Graphical view ]
SUPFAMi SSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 5 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 2 hits.
TIGRFAMsi TIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEi PS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.
  3. "Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain."
    Henneke G., Gueguen Y., Flament D., Azam P., Querellou J., Dietrich J., Huebscher U., Raffin J.-P.
    J. Mol. Biol. 323:795-810(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, SUBUNIT.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiRFCS_PYRAB
AccessioniPrimary (citable) accession number: Q9V2G4
Secondary accession number(s): G8ZFU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the potential ATP-binding site.

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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