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Q9V2G4

- RFCS_PYRAB

UniProt

Q9V2G4 - RFCS_PYRAB

Protein

Replication factor C small subunit

Gene

rfcS

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-independent ATPase activity.1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. endonuclease activity Source: InterPro
    3. four-way junction helicase activity Source: InterPro
    4. sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: InterPro
    2. DNA repair Source: InterPro
    3. DNA replication Source: UniProtKB-KW
    4. intein-mediated protein splicing Source: InterPro

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replication factor C small subunit
    Short name:
    RFC small subunit
    Alternative name(s):
    Clamp loader small subunit
    PabRFC small subunit
    Cleaved into the following 2 chains:
    Gene namesi
    Name:rfcS
    Ordered Locus Names:PYRAB01100
    ORF Names:PAB0068
    OrganismiPyrococcus abyssi (strain GE5 / Orsay)
    Taxonomic identifieri272844 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000810: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6161Replication factor C small subunit, 1st partSequence AnalysisPRO_0000030371Add
    BLAST
    Chaini62 – 560499Pab RFC-1 inteinSequence AnalysisPRO_0000030372Add
    BLAST
    Chaini561 – 64787Replication factor C small subunit, 2nd partSequence AnalysisPRO_0000030373Add
    BLAST
    Chaini648 – 1255608Pab RFC-2 inteinSequence AnalysisPRO_0000030374Add
    BLAST
    Chaini1256 – 1437182Replication factor C small subunit, 3rd partSequence AnalysisPRO_0000030375Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Heterohexamer composed of four small subunits (RfcS) and two large subunits (RfcL).1 Publication

    Protein-protein interaction databases

    IntActiQ9V2G4. 1 interaction.
    MINTiMINT-7906090.
    STRINGi272844.PAB0068.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9V2G4.
    SMRiQ9V2G4. Positions 5-61, 524-668, 1246-1436.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini185 – 301117DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini940 – 1098159DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 DOD-type homing endonuclease domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1372.
    HOGENOMiHOG000154102.
    KOiK04801.
    OMAiTNDENEM.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    1.10.260.40. 1 hit.
    2.170.16.10. 6 hits.
    3.10.28.10. 2 hits.
    3.40.50.300. 3 hits.
    InterProiIPR003959. ATPase_AAA_core.
    IPR001387. Cro/C1-type_HTH.
    IPR008824. DNA_helicase_Holl-junc_RuvB_N.
    IPR008921. DNA_pol3_clamp-load_cplx_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR010982. Lambda_DNA-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR013748. Rep_factorC_C_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    PF01381. HTH_3. 1 hit.
    PF14528. LAGLIDADG_3. 2 hits.
    PF08542. Rep_fac_C. 1 hit.
    PF05496. RuvB_N. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 2 hits.
    SM00306. HintN. 2 hits.
    SM00530. HTH_XRE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47413. SSF47413. 1 hit.
    SSF48019. SSF48019. 1 hit.
    SSF51294. SSF51294. 5 hits.
    SSF52540. SSF52540. 3 hits.
    SSF55608. SSF55608. 2 hits.
    TIGRFAMsiTIGR01443. intein_Cterm. 2 hits.
    TIGR01445. intein_Nterm. 2 hits.
    PROSITEiPS50818. INTEIN_C_TER. 2 hits.
    PS50819. INTEIN_ENDONUCLEASE. 2 hits.
    PS50817. INTEIN_N_TER. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9V2G4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRDMEEVREV KVLEKPWVEK YRPQKLEEIV GQEHIVKRLK HYVKTGSMPH     50
    LLFAGPPGVG KCLTGDAKVI ANGELTTIGE LVERISNGKL GPTPVRGLTV 100
    LGIDEDGKLV ELPVEYVYKD KTSELVKIRT RLGRELKVTP YHPLLVNRRN 150
    GKIEWVKAEE LKPGDRLAIP SFLPAMLNDN PLAEWLGYFF GNGYTDSEER 200
    VVFESKSKEL RKRFMELTRK LFQDAEIKED SGKVYVSSSE VKRLVKSLNK 250
    DSIPEQAWKG LRSFLRAYFD CNAEIKDKII VSTAGKEIAE QISYALAGLG 300
    IVAEVDDKGS VIISDPENVS RFLDEIGFSV EEKKEEAKAL IKKSTLNLGI 350
    YVDKELISYV REKLKLSFYE NETMWSPEKA REIAWKLMKE IYYRLDELER 400
    FKKALSKSVI IDWSEVEKKK EEISEKTGIS VNEILEYAKG KRKPSLEEYV 450
    KIAKALGVEL KETLEAIFTF GKKYLGYVIS DEIETLEEVR KEELKRLKEL 500
    LNDEKLKKGV AYLIFLAQNE LLWDEIIEVE KLKGDFVIYD LHVPKYHNFI 550
    GGNLPTVLHN TTAALALARE LFGENWRHNF LELNASDERG INVIREKVKE 600
    FARTKPIGGA SFKIIFLDEA DALTQDAQQA LRRTMEMFSS NVRFILSCVT 650
    GDTKVYTPDE REVKIRDFMN YFENGLIKEV SNRIGRDTVI AAVSFNSRIV 700
    GHPVYRLTLE SGRIIEATGD HMFLTPEGWK QTYDIKEGSE VLVKPTLEGT 750
    PYEPDPRVII DIKEFYNFLE KIEREHNLKP LKEAKTFREL ITKDKEKILR 800
    RALELRAEIE NGLTKREAEI LELISADTWI PRAELEKKAR ISRTRLNQIL 850
    QRLEKKGYIE RRIEGRKQFV RKIRNGKILR NAMDIKRILE EEFGIKISYT 900
    TVKKLLSGNV DGMAYRILKE VKEKWLVRYD DEKAGILARV VGFILGDGHL 950
    ARNGRIWFNS SKEELEMLAN DLRKLGLKPS EIIERDSSSE IQGRKVKGRI 1000
    YMLYVDNAAF HALLRFWKVE VGNKTKKGYT VPEWIKKGNL FVKREFLRGL 1050
    FGADGTKPCG KRYNFNGIKL EIRAKKESLE RTVEFLNDVA DLLREFDVDS 1100
    KITVSPTKEG FIIRLIVTPN DANYLNFLTR VGYAYAKDTY ARLVGEYIRI 1150
    KLAYKNIILP GIAEKAIELA TVTNSTYAAK VLGVSRDFVV NRLKGTQIGI 1200
    TRDFMTFEEF MKERVLNGYV IEKVIKKEKL GYLDVYDVTC ARDHSFISNG 1250
    LVSHNCNYSS KIIEPIQSRC AIFRFRPLND EDIAKRLRYI AENEGLELTE 1300
    EGLQAILYIA EGDMRRAINI LQAAAALDRK ITDENVFLVA SRARPEDIRE 1350
    MMLLALKGNF LKAREKLREI LLKQGLSGED VLVQMHREVF NLPIDEPMKV 1400
    YLADKIGEYN FRLVEGANEM IQLEALLAQF TLIGKKK 1437
    Length:1,437
    Mass (Da):165,246
    Last modified:May 1, 2000 - v1
    Checksum:i9725AC4F4240C985
    GO

    Sequence cautioni

    The sequence CCE69486.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ248283 Genomic DNA. Translation: CAB49034.1.
    HE613800 Genomic DNA. Translation: CCE69486.1. Different initiation.
    PIRiC75198.
    RefSeqiNP_125803.1. NC_000868.1.

    Genome annotation databases

    EnsemblBacteriaiCAB49034; CAB49034; PAB0068.
    GeneIDi1494997.
    KEGGipab:PAB0068.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ248283 Genomic DNA. Translation: CAB49034.1 .
    HE613800 Genomic DNA. Translation: CCE69486.1 . Different initiation.
    PIRi C75198.
    RefSeqi NP_125803.1. NC_000868.1.

    3D structure databases

    ProteinModelPortali Q9V2G4.
    SMRi Q9V2G4. Positions 5-61, 524-668, 1246-1436.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9V2G4. 1 interaction.
    MINTi MINT-7906090.
    STRINGi 272844.PAB0068.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB49034 ; CAB49034 ; PAB0068 .
    GeneIDi 1494997.
    KEGGi pab:PAB0068.

    Phylogenomic databases

    eggNOGi COG1372.
    HOGENOMi HOG000154102.
    KOi K04801.
    OMAi TNDENEM.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    1.10.260.40. 1 hit.
    2.170.16.10. 6 hits.
    3.10.28.10. 2 hits.
    3.40.50.300. 3 hits.
    InterProi IPR003959. ATPase_AAA_core.
    IPR001387. Cro/C1-type_HTH.
    IPR008824. DNA_helicase_Holl-junc_RuvB_N.
    IPR008921. DNA_pol3_clamp-load_cplx_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR010982. Lambda_DNA-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR013748. Rep_factorC_C_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    PF01381. HTH_3. 1 hit.
    PF14528. LAGLIDADG_3. 2 hits.
    PF08542. Rep_fac_C. 1 hit.
    PF05496. RuvB_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 2 hits.
    SM00306. HintN. 2 hits.
    SM00530. HTH_XRE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47413. SSF47413. 1 hit.
    SSF48019. SSF48019. 1 hit.
    SSF51294. SSF51294. 5 hits.
    SSF52540. SSF52540. 3 hits.
    SSF55608. SSF55608. 2 hits.
    TIGRFAMsi TIGR01443. intein_Cterm. 2 hits.
    TIGR01445. intein_Nterm. 2 hits.
    PROSITEi PS50818. INTEIN_C_TER. 2 hits.
    PS50819. INTEIN_ENDONUCLEASE. 2 hits.
    PS50817. INTEIN_N_TER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GE5 / Orsay.
    2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
      Gao J., Wang J.
      Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: GE5 / Orsay.
    3. "Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain."
      Henneke G., Gueguen Y., Flament D., Azam P., Querellou J., Dietrich J., Huebscher U., Raffin J.-P.
      J. Mol. Biol. 323:795-810(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, SUBUNIT.
      Strain: GE5 / Orsay.

    Entry informationi

    Entry nameiRFCS_PYRAB
    AccessioniPrimary (citable) accession number: Q9V2G4
    Secondary accession number(s): G8ZFU5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 16, 2004
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The intein interrupts the potential ATP-binding site.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3