Q9V2G4 (RFCS_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replication factor C small subunit
Short name=RFC small subunit

Alternative name(s):
Clamp loader small subunit
PabRFC small subunit

Cleaved into the following 2 chains:

1. Pab RFC-1 intein
2. Pab RFC-2 intein

Gene names

Name:	rfcS
Ordered Locus Names:	PYRAB01100
ORF Names:	PAB0068

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus abyssi

Protein attributes

Sequence length	1437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-independent ATPase activity. Ref.3
Subunit structure	Heterohexamer composed of four small subunits (RfcS) and two large subunits (RfcL) Probable. Ref.3
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_01509
Miscellaneous	The intein interrupts the potential ATP-binding site. HAMAP-Rule MF_01509
Sequence similarities	Belongs to the activator 1 small subunits family. RfcS subfamily. Contains 2 DOD-type homing endonuclease domains.
Sequence caution	The sequence CCE69486.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	DNA replication
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Replication factor C small subunit, 1st part Potential		PRO_0000030371
	Chain	62 – 560	499	Pab RFC-1 intein Potential		PRO_0000030372
	Chain	561 – 647	87	Replication factor C small subunit, 2nd part Potential		PRO_0000030373
	Chain	648 – 1255	608	Pab RFC-2 intein Potential		PRO_0000030374
	Chain	1256 – 1437	182	Replication factor C small subunit, 3rd part Potential		PRO_0000030375
Regions
	Domain	185 – 301	117	DOD-type homing endonuclease 1
	Domain	940 – 1098	159	DOD-type homing endonuclease 2

Sequences

	Sequence		Length	Mass (Da)
	Q9V2G4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 9725AC4F4240C985	FASTA	1,437	165,246
	10 20 30 40 50 60 MRDMEEVREV KVLEKPWVEK YRPQKLEEIV GQEHIVKRLK HYVKTGSMPH LLFAGPPGVG 70 80 90 100 110 120 KCLTGDAKVI ANGELTTIGE LVERISNGKL GPTPVRGLTV LGIDEDGKLV ELPVEYVYKD 130 140 150 160 170 180 KTSELVKIRT RLGRELKVTP YHPLLVNRRN GKIEWVKAEE LKPGDRLAIP SFLPAMLNDN 190 200 210 220 230 240 PLAEWLGYFF GNGYTDSEER VVFESKSKEL RKRFMELTRK LFQDAEIKED SGKVYVSSSE 250 260 270 280 290 300 VKRLVKSLNK DSIPEQAWKG LRSFLRAYFD CNAEIKDKII VSTAGKEIAE QISYALAGLG 310 320 330 340 350 360 IVAEVDDKGS VIISDPENVS RFLDEIGFSV EEKKEEAKAL IKKSTLNLGI YVDKELISYV 370 380 390 400 410 420 REKLKLSFYE NETMWSPEKA REIAWKLMKE IYYRLDELER FKKALSKSVI IDWSEVEKKK 430 440 450 460 470 480 EEISEKTGIS VNEILEYAKG KRKPSLEEYV KIAKALGVEL KETLEAIFTF GKKYLGYVIS 490 500 510 520 530 540 DEIETLEEVR KEELKRLKEL LNDEKLKKGV AYLIFLAQNE LLWDEIIEVE KLKGDFVIYD 550 560 570 580 590 600 LHVPKYHNFI GGNLPTVLHN TTAALALARE LFGENWRHNF LELNASDERG INVIREKVKE 610 620 630 640 650 660 FARTKPIGGA SFKIIFLDEA DALTQDAQQA LRRTMEMFSS NVRFILSCVT GDTKVYTPDE 670 680 690 700 710 720 REVKIRDFMN YFENGLIKEV SNRIGRDTVI AAVSFNSRIV GHPVYRLTLE SGRIIEATGD 730 740 750 760 770 780 HMFLTPEGWK QTYDIKEGSE VLVKPTLEGT PYEPDPRVII DIKEFYNFLE KIEREHNLKP 790 800 810 820 830 840 LKEAKTFREL ITKDKEKILR RALELRAEIE NGLTKREAEI LELISADTWI PRAELEKKAR 850 860 870 880 890 900 ISRTRLNQIL QRLEKKGYIE RRIEGRKQFV RKIRNGKILR NAMDIKRILE EEFGIKISYT 910 920 930 940 950 960 TVKKLLSGNV DGMAYRILKE VKEKWLVRYD DEKAGILARV VGFILGDGHL ARNGRIWFNS 970 980 990 1000 1010 1020 SKEELEMLAN DLRKLGLKPS EIIERDSSSE IQGRKVKGRI YMLYVDNAAF HALLRFWKVE 1030 1040 1050 1060 1070 1080 VGNKTKKGYT VPEWIKKGNL FVKREFLRGL FGADGTKPCG KRYNFNGIKL EIRAKKESLE 1090 1100 1110 1120 1130 1140 RTVEFLNDVA DLLREFDVDS KITVSPTKEG FIIRLIVTPN DANYLNFLTR VGYAYAKDTY 1150 1160 1170 1180 1190 1200 ARLVGEYIRI KLAYKNIILP GIAEKAIELA TVTNSTYAAK VLGVSRDFVV NRLKGTQIGI 1210 1220 1230 1240 1250 1260 TRDFMTFEEF MKERVLNGYV IEKVIKKEKL GYLDVYDVTC ARDHSFISNG LVSHNCNYSS 1270 1280 1290 1300 1310 1320 KIIEPIQSRC AIFRFRPLND EDIAKRLRYI AENEGLELTE EGLQAILYIA EGDMRRAINI 1330 1340 1350 1360 1370 1380 LQAAAALDRK ITDENVFLVA SRARPEDIRE MMLLALKGNF LKAREKLREI LLKQGLSGED 1390 1400 1410 1420 1430 VLVQMHREVF NLPIDEPMKV YLADKIGEYN FRLVEGANEM IQLEALLAQF TLIGKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.
[2]	"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638." Gao J., Wang J. Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: GE5 / Orsay.
[3]	"Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain." Henneke G., Gueguen Y., Flament D., Azam P., Querellou J., Dietrich J., Huebscher U., Raffin J.-P. J. Mol. Biol. 323:795-810(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, SUBUNIT. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248283 Genomic DNA. Translation: CAB49034.1. HE613800 Genomic DNA. Translation: CCE69486.1. Different initiation.
PIR	C75198.
RefSeq	NP_125803.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V2G4.
SMR	Q9V2G4. Positions 5-61, 524-668, 1246-1436.
ModBase	Search...
Protein-protein interaction databases
STRING	272844.PAB0068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB49034; CAB49034; PAB0068.
GeneID	1494997.
KEGG	pab:PAB0068.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1372.
HOGENOM	HOG000154102.
KO	K04801.
OMA	YDDEKAG.
ProtClustDB	PRK04132.
Family and domain databases
Gene3D	1.10.10.10. 1 hit. 1.10.260.40. 1 hit.
HAMAP	MF_01509. RfcS. Divergent sequence.
InterPro	IPR003959. ATPase_AAA_core. IPR008824. DNA_helicase_Holl-junc_RuvB_N. IPR008921. DNA_pol3_clamp-load_cplx_C. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR001387. HTH. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR010982. Lambda_DNA-bd_dom. IPR013748. Rep_factorC_C_dom. IPR002831. Tscrpt_reg_TrmB_N. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF01381. HTH_3. 1 hit. PF08542. Rep_fac_C. 1 hit. PF05496. RuvB_N. 1 hit. PF01978. TrmB. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 2 hits. SM00306. HintN. 2 hits. SM00530. HTH_XRE. 1 hit. [Graphical view]
SUPFAM	SSF47413. Lambda_like_DNA. 1 hit. SSF48019. Pol_clamp_load_C. 1 hit.
TIGRFAMs	TIGR01443. intein_Cterm. 2 hits. TIGR01445. intein_Nterm. 2 hits.
PROSITE	PS50818. INTEIN_C_TER. 2 hits. PS50819. INTEIN_ENDONUCLEASE. 2 hits. PS50817. INTEIN_N_TER. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFCS_PYRAB

Accession

Primary (citable) accession number: Q9V2G4
Secondary accession number(s): G8ZFU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program