Reviewed, UniProtKB/Swiss-Prot Q9V2G4 (RFCS_PYRAB)

Names and origin

Protein names

Recommended name:
    Replication factor C small subunit
      Short name=RFC small subunit
Alternative name(s):
    Clamp loader small subunit
    PabRFC small subunit
Cleaved into the following 2 chains:
    1- Recommended name:
            Pab RFC-1 intein
    2- Recommended name:
            Pab RFC-2 intein

Gene names

Name:	rfcS
Ordered Locus Names:	PYRAB01100
ORF Names:	PAB0068

Organism

Pyrococcus abyssi [Complete proteome] [HAMAP]

Taxonomic identifier

29292 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	1437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-independent ATPase activity. Ref.2
Subunit structure	Heterohexamer composed of four small subunits (rfcS) and two large subunits (rfcL) Probable.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Miscellaneous	The intein interrupts the potential ATP-binding site. HAMAP MF_01509
Sequence similarities	Belongs to the activator 1 small subunits family. RfcS subfamily. Contains 2 DOD-type homing endonuclease domains.

Ontologies

Keywords
Biological process	DNA replication
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	DNA replication factor C complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA clamp loader activity Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Replication factor C small subunit, 1st part Potential		PRO_0000030371
	Chain	62 – 560	499	Pab RFC-1 intein Potential		PRO_0000030372
	Chain	561 – 647	87	Replication factor C small subunit, 2nd part Potential		PRO_0000030373
	Chain	648 – 1255	608	Pab RFC-2 intein Potential		PRO_0000030374
	Chain	1256 – 1437	182	Replication factor C small subunit, 3rd part Potential		PRO_0000030375
Regions
	Domain	185 – 301	117	DOD-type homing endonuclease 1
	Domain	940 – 1098	159	DOD-type homing endonuclease 2

Sequences

	Sequence		Length	Mass (Da)
	Q9V2G4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 9725AC4F4240C985	FASTA	1,437	165,246
	10 20 30 40 50 60 MRDMEEVREV KVLEKPWVEK YRPQKLEEIV GQEHIVKRLK HYVKTGSMPH LLFAGPPGVG 70 80 90 100 110 120 KCLTGDAKVI ANGELTTIGE LVERISNGKL GPTPVRGLTV LGIDEDGKLV ELPVEYVYKD 130 140 150 160 170 180 KTSELVKIRT RLGRELKVTP YHPLLVNRRN GKIEWVKAEE LKPGDRLAIP SFLPAMLNDN 190 200 210 220 230 240 PLAEWLGYFF GNGYTDSEER VVFESKSKEL RKRFMELTRK LFQDAEIKED SGKVYVSSSE 250 260 270 280 290 300 VKRLVKSLNK DSIPEQAWKG LRSFLRAYFD CNAEIKDKII VSTAGKEIAE QISYALAGLG 310 320 330 340 350 360 IVAEVDDKGS VIISDPENVS RFLDEIGFSV EEKKEEAKAL IKKSTLNLGI YVDKELISYV 370 380 390 400 410 420 REKLKLSFYE NETMWSPEKA REIAWKLMKE IYYRLDELER FKKALSKSVI IDWSEVEKKK 430 440 450 460 470 480 EEISEKTGIS VNEILEYAKG KRKPSLEEYV KIAKALGVEL KETLEAIFTF GKKYLGYVIS 490 500 510 520 530 540 DEIETLEEVR KEELKRLKEL LNDEKLKKGV AYLIFLAQNE LLWDEIIEVE KLKGDFVIYD 550 560 570 580 590 600 LHVPKYHNFI GGNLPTVLHN TTAALALARE LFGENWRHNF LELNASDERG INVIREKVKE 610 620 630 640 650 660 FARTKPIGGA SFKIIFLDEA DALTQDAQQA LRRTMEMFSS NVRFILSCVT GDTKVYTPDE 670 680 690 700 710 720 REVKIRDFMN YFENGLIKEV SNRIGRDTVI AAVSFNSRIV GHPVYRLTLE SGRIIEATGD 730 740 750 760 770 780 HMFLTPEGWK QTYDIKEGSE VLVKPTLEGT PYEPDPRVII DIKEFYNFLE KIEREHNLKP 790 800 810 820 830 840 LKEAKTFREL ITKDKEKILR RALELRAEIE NGLTKREAEI LELISADTWI PRAELEKKAR 850 860 870 880 890 900 ISRTRLNQIL QRLEKKGYIE RRIEGRKQFV RKIRNGKILR NAMDIKRILE EEFGIKISYT 910 920 930 940 950 960 TVKKLLSGNV DGMAYRILKE VKEKWLVRYD DEKAGILARV VGFILGDGHL ARNGRIWFNS 970 980 990 1000 1010 1020 SKEELEMLAN DLRKLGLKPS EIIERDSSSE IQGRKVKGRI YMLYVDNAAF HALLRFWKVE 1030 1040 1050 1060 1070 1080 VGNKTKKGYT VPEWIKKGNL FVKREFLRGL FGADGTKPCG KRYNFNGIKL EIRAKKESLE 1090 1100 1110 1120 1130 1140 RTVEFLNDVA DLLREFDVDS KITVSPTKEG FIIRLIVTPN DANYLNFLTR VGYAYAKDTY 1150 1160 1170 1180 1190 1200 ARLVGEYIRI KLAYKNIILP GIAEKAIELA TVTNSTYAAK VLGVSRDFVV NRLKGTQIGI 1210 1220 1230 1240 1250 1260 TRDFMTFEEF MKERVLNGYV IEKVIKKEKL GYLDVYDVTC ARDHSFISNG LVSHNCNYSS 1270 1280 1290 1300 1310 1320 KIIEPIQSRC AIFRFRPLND EDIAKRLRYI AENEGLELTE EGLQAILYIA EGDMRRAINI 1330 1340 1350 1360 1370 1380 LQAAAALDRK ITDENVFLVA SRARPEDIRE MMLLALKGNF LKAREKLREI LLKQGLSGED 1390 1400 1410 1420 1430 VLVQMHREVF NLPIDEPMKV YLADKIGEYN FRLVEGANEM IQLEALLAQF TLIGKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.
[2]	"Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain." Henneke G., Gueguen Y., Flament D., Azam P., Querellou J., Dietrich J., Huebscher U., Raffin J.-P. J. Mol. Biol. 323:795-810(2002) [PubMed: 12417194] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, SUBUNIT. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ248283 Genomic DNA. Translation: CAB49034.1.
PIR	C75198.
RefSeq	NP_125803.1.
3D structure databases
SMR	Q9V2G4. Positions 5-61, 555-668, 1246-1436.
ModBase	Search...
Genome annotation databases
GeneID	1494997.
GenomeReviews	Gene locus PYRAB01100 in contig AL096836_GR.
KEGG	pab:PAB0068.
NMPDR	fig|272844.1.peg.121.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9V2G4.
OMA	YDDEKAG
Enzyme and pathway databases
BioCyc	PABY272844:PAB0068-MON.
Family and domain databases
HAMAP	MF_01509. Divergent sequence. [Tree]
InterPro	IPR008921. DNA_pol3_clamp-load_cplx_C. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR001387. HTH_3. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR010982. Lambda_DNA-bd. IPR013748. Replication_factor_C. IPR002831. TrmB_Trans_reg. [Graphical view]
Pfam	PF01381. HTH_3. 1 hit. PF08542. Rep_fac_C. 1 hit. PF01978. TrmB. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 2 hits. SM00306. HintN. 2 hits. SM00530. HTH_XRE. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 2 hits. TIGR01445. intein_Nterm. 2 hits.
PROSITE	PS50818. INTEIN_C_TER. 2 hits. PS50819. INTEIN_ENDONUCLEASE. 2 hits. PS50817. INTEIN_N_TER. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFCS_PYRAB

Accession

Primary (citable) accession number: Q9V2G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	May 1, 2000
Last modified:	November 24, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)