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Q9V2G4 (RFCS_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replication factor C small subunit

Short name=RFC small subunit
Alternative name(s):
Clamp loader small subunit
PabRFC small subunit

Cleaved into the following 2 chains:

  1. Pab RFC-1 intein
  2. Pab RFC-2 intein
Gene names
Name:rfcS
Ordered Locus Names:PYRAB01100
ORF Names:PAB0068
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length1437 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-independent ATPase activity. Ref.3

Subunit structure

Heterohexamer composed of four small subunits (RfcS) and two large subunits (RfcL) Probable. Ref.3

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Miscellaneous

The intein interrupts the potential ATP-binding site.

Sequence similarities

Belongs to the activator 1 small subunits family. RfcS subfamily.

Contains 2 DOD-type homing endonuclease domains.

Sequence caution

The sequence CCE69486.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6161Replication factor C small subunit, 1st part Potential
PRO_0000030371
Chain62 – 560499Pab RFC-1 intein Potential
PRO_0000030372
Chain561 – 64787Replication factor C small subunit, 2nd part Potential
PRO_0000030373
Chain648 – 1255608Pab RFC-2 intein Potential
PRO_0000030374
Chain1256 – 1437182Replication factor C small subunit, 3rd part Potential
PRO_0000030375

Regions

Domain185 – 301117DOD-type homing endonuclease 1
Domain940 – 1098159DOD-type homing endonuclease 2

Sequences

Sequence LengthMass (Da)Tools
Q9V2G4 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 9725AC4F4240C985

FASTA1,437165,246
        10         20         30         40         50         60 
MRDMEEVREV KVLEKPWVEK YRPQKLEEIV GQEHIVKRLK HYVKTGSMPH LLFAGPPGVG 

        70         80         90        100        110        120 
KCLTGDAKVI ANGELTTIGE LVERISNGKL GPTPVRGLTV LGIDEDGKLV ELPVEYVYKD 

       130        140        150        160        170        180 
KTSELVKIRT RLGRELKVTP YHPLLVNRRN GKIEWVKAEE LKPGDRLAIP SFLPAMLNDN 

       190        200        210        220        230        240 
PLAEWLGYFF GNGYTDSEER VVFESKSKEL RKRFMELTRK LFQDAEIKED SGKVYVSSSE 

       250        260        270        280        290        300 
VKRLVKSLNK DSIPEQAWKG LRSFLRAYFD CNAEIKDKII VSTAGKEIAE QISYALAGLG 

       310        320        330        340        350        360 
IVAEVDDKGS VIISDPENVS RFLDEIGFSV EEKKEEAKAL IKKSTLNLGI YVDKELISYV 

       370        380        390        400        410        420 
REKLKLSFYE NETMWSPEKA REIAWKLMKE IYYRLDELER FKKALSKSVI IDWSEVEKKK 

       430        440        450        460        470        480 
EEISEKTGIS VNEILEYAKG KRKPSLEEYV KIAKALGVEL KETLEAIFTF GKKYLGYVIS 

       490        500        510        520        530        540 
DEIETLEEVR KEELKRLKEL LNDEKLKKGV AYLIFLAQNE LLWDEIIEVE KLKGDFVIYD 

       550        560        570        580        590        600 
LHVPKYHNFI GGNLPTVLHN TTAALALARE LFGENWRHNF LELNASDERG INVIREKVKE 

       610        620        630        640        650        660 
FARTKPIGGA SFKIIFLDEA DALTQDAQQA LRRTMEMFSS NVRFILSCVT GDTKVYTPDE 

       670        680        690        700        710        720 
REVKIRDFMN YFENGLIKEV SNRIGRDTVI AAVSFNSRIV GHPVYRLTLE SGRIIEATGD 

       730        740        750        760        770        780 
HMFLTPEGWK QTYDIKEGSE VLVKPTLEGT PYEPDPRVII DIKEFYNFLE KIEREHNLKP 

       790        800        810        820        830        840 
LKEAKTFREL ITKDKEKILR RALELRAEIE NGLTKREAEI LELISADTWI PRAELEKKAR 

       850        860        870        880        890        900 
ISRTRLNQIL QRLEKKGYIE RRIEGRKQFV RKIRNGKILR NAMDIKRILE EEFGIKISYT 

       910        920        930        940        950        960 
TVKKLLSGNV DGMAYRILKE VKEKWLVRYD DEKAGILARV VGFILGDGHL ARNGRIWFNS 

       970        980        990       1000       1010       1020 
SKEELEMLAN DLRKLGLKPS EIIERDSSSE IQGRKVKGRI YMLYVDNAAF HALLRFWKVE 

      1030       1040       1050       1060       1070       1080 
VGNKTKKGYT VPEWIKKGNL FVKREFLRGL FGADGTKPCG KRYNFNGIKL EIRAKKESLE 

      1090       1100       1110       1120       1130       1140 
RTVEFLNDVA DLLREFDVDS KITVSPTKEG FIIRLIVTPN DANYLNFLTR VGYAYAKDTY 

      1150       1160       1170       1180       1190       1200 
ARLVGEYIRI KLAYKNIILP GIAEKAIELA TVTNSTYAAK VLGVSRDFVV NRLKGTQIGI 

      1210       1220       1230       1240       1250       1260 
TRDFMTFEEF MKERVLNGYV IEKVIKKEKL GYLDVYDVTC ARDHSFISNG LVSHNCNYSS 

      1270       1280       1290       1300       1310       1320 
KIIEPIQSRC AIFRFRPLND EDIAKRLRYI AENEGLELTE EGLQAILYIA EGDMRRAINI 

      1330       1340       1350       1360       1370       1380 
LQAAAALDRK ITDENVFLVA SRARPEDIRE MMLLALKGNF LKAREKLREI LLKQGLSGED 

      1390       1400       1410       1420       1430 
VLVQMHREVF NLPIDEPMKV YLADKIGEYN FRLVEGANEM IQLEALLAQF TLIGKKK 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.
[3]"Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain."
Henneke G., Gueguen Y., Flament D., Azam P., Querellou J., Dietrich J., Huebscher U., Raffin J.-P.
J. Mol. Biol. 323:795-810(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, SUBUNIT.
Strain: GE5 / Orsay.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248283 Genomic DNA. Translation: CAB49034.1.
HE613800 Genomic DNA. Translation: CCE69486.1. Different initiation.
PIRC75198.
RefSeqNP_125803.1. NC_000868.1.

3D structure databases

ProteinModelPortalQ9V2G4.
SMRQ9V2G4. Positions 5-61, 524-668, 1246-1436.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9V2G4. 1 interaction.
MINTMINT-7906090.
STRING272844.PAB0068.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB49034; CAB49034; PAB0068.
GeneID1494997.
KEGGpab:PAB0068.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1372.
HOGENOMHOG000154102.
KOK04801.
OMATNDENEM.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.10.260.40. 1 hit.
2.170.16.10. 6 hits.
3.10.28.10. 2 hits.
3.40.50.300. 3 hits.
InterProIPR003959. ATPase_AAA_core.
IPR001387. Cro/C1-type_HTH.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00004. AAA. 1 hit.
PF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 2 hits.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMSSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 5 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 2 hits.
TIGRFAMsTIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEPS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFCS_PYRAB
AccessionPrimary (citable) accession number: Q9V2G4
Secondary accession number(s): G8ZFU5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries