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Q9V2G4

- RFCS_PYRAB

UniProt

Q9V2G4 - RFCS_PYRAB

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Protein

Replication factor C small subunit

Gene

rfcS

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-independent ATPase activity.1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. endonuclease activity Source: InterPro
  3. four-way junction helicase activity Source: InterPro
  4. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: InterPro
  2. DNA repair Source: InterPro
  3. DNA replication Source: UniProtKB-KW
  4. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C small subunit
Short name:
RFC small subunit
Alternative name(s):
Clamp loader small subunit
PabRFC small subunit
Cleaved into the following 2 chains:
Gene namesi
Name:rfcS
Ordered Locus Names:PYRAB01100
ORF Names:PAB0068
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000810: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6161Replication factor C small subunit, 1st partSequence AnalysisPRO_0000030371Add
BLAST
Chaini62 – 560499Pab RFC-1 inteinSequence AnalysisPRO_0000030372Add
BLAST
Chaini561 – 64787Replication factor C small subunit, 2nd partSequence AnalysisPRO_0000030373Add
BLAST
Chaini648 – 1255608Pab RFC-2 inteinSequence AnalysisPRO_0000030374Add
BLAST
Chaini1256 – 1437182Replication factor C small subunit, 3rd partSequence AnalysisPRO_0000030375Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Heterohexamer composed of four small subunits (RfcS) and two large subunits (RfcL).1 Publication

Protein-protein interaction databases

IntActiQ9V2G4. 1 interaction.
MINTiMINT-7906090.
STRINGi272844.PAB0068.

Structurei

3D structure databases

ProteinModelPortaliQ9V2G4.
SMRiQ9V2G4. Positions 5-61, 524-668, 1246-1436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini185 – 301117DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd
BLAST
Domaini940 – 1098159DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 DOD-type homing endonuclease domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000154102.
KOiK04801.
OMAiTNDENEM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.260.40. 1 hit.
2.170.16.10. 6 hits.
3.10.28.10. 2 hits.
3.40.50.300. 3 hits.
InterProiIPR003959. ATPase_AAA_core.
IPR001387. Cro/C1-type_HTH.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 2 hits.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 5 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEiPS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9V2G4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRDMEEVREV KVLEKPWVEK YRPQKLEEIV GQEHIVKRLK HYVKTGSMPH
60 70 80 90 100
LLFAGPPGVG KCLTGDAKVI ANGELTTIGE LVERISNGKL GPTPVRGLTV
110 120 130 140 150
LGIDEDGKLV ELPVEYVYKD KTSELVKIRT RLGRELKVTP YHPLLVNRRN
160 170 180 190 200
GKIEWVKAEE LKPGDRLAIP SFLPAMLNDN PLAEWLGYFF GNGYTDSEER
210 220 230 240 250
VVFESKSKEL RKRFMELTRK LFQDAEIKED SGKVYVSSSE VKRLVKSLNK
260 270 280 290 300
DSIPEQAWKG LRSFLRAYFD CNAEIKDKII VSTAGKEIAE QISYALAGLG
310 320 330 340 350
IVAEVDDKGS VIISDPENVS RFLDEIGFSV EEKKEEAKAL IKKSTLNLGI
360 370 380 390 400
YVDKELISYV REKLKLSFYE NETMWSPEKA REIAWKLMKE IYYRLDELER
410 420 430 440 450
FKKALSKSVI IDWSEVEKKK EEISEKTGIS VNEILEYAKG KRKPSLEEYV
460 470 480 490 500
KIAKALGVEL KETLEAIFTF GKKYLGYVIS DEIETLEEVR KEELKRLKEL
510 520 530 540 550
LNDEKLKKGV AYLIFLAQNE LLWDEIIEVE KLKGDFVIYD LHVPKYHNFI
560 570 580 590 600
GGNLPTVLHN TTAALALARE LFGENWRHNF LELNASDERG INVIREKVKE
610 620 630 640 650
FARTKPIGGA SFKIIFLDEA DALTQDAQQA LRRTMEMFSS NVRFILSCVT
660 670 680 690 700
GDTKVYTPDE REVKIRDFMN YFENGLIKEV SNRIGRDTVI AAVSFNSRIV
710 720 730 740 750
GHPVYRLTLE SGRIIEATGD HMFLTPEGWK QTYDIKEGSE VLVKPTLEGT
760 770 780 790 800
PYEPDPRVII DIKEFYNFLE KIEREHNLKP LKEAKTFREL ITKDKEKILR
810 820 830 840 850
RALELRAEIE NGLTKREAEI LELISADTWI PRAELEKKAR ISRTRLNQIL
860 870 880 890 900
QRLEKKGYIE RRIEGRKQFV RKIRNGKILR NAMDIKRILE EEFGIKISYT
910 920 930 940 950
TVKKLLSGNV DGMAYRILKE VKEKWLVRYD DEKAGILARV VGFILGDGHL
960 970 980 990 1000
ARNGRIWFNS SKEELEMLAN DLRKLGLKPS EIIERDSSSE IQGRKVKGRI
1010 1020 1030 1040 1050
YMLYVDNAAF HALLRFWKVE VGNKTKKGYT VPEWIKKGNL FVKREFLRGL
1060 1070 1080 1090 1100
FGADGTKPCG KRYNFNGIKL EIRAKKESLE RTVEFLNDVA DLLREFDVDS
1110 1120 1130 1140 1150
KITVSPTKEG FIIRLIVTPN DANYLNFLTR VGYAYAKDTY ARLVGEYIRI
1160 1170 1180 1190 1200
KLAYKNIILP GIAEKAIELA TVTNSTYAAK VLGVSRDFVV NRLKGTQIGI
1210 1220 1230 1240 1250
TRDFMTFEEF MKERVLNGYV IEKVIKKEKL GYLDVYDVTC ARDHSFISNG
1260 1270 1280 1290 1300
LVSHNCNYSS KIIEPIQSRC AIFRFRPLND EDIAKRLRYI AENEGLELTE
1310 1320 1330 1340 1350
EGLQAILYIA EGDMRRAINI LQAAAALDRK ITDENVFLVA SRARPEDIRE
1360 1370 1380 1390 1400
MMLLALKGNF LKAREKLREI LLKQGLSGED VLVQMHREVF NLPIDEPMKV
1410 1420 1430
YLADKIGEYN FRLVEGANEM IQLEALLAQF TLIGKKK
Length:1,437
Mass (Da):165,246
Last modified:May 1, 2000 - v1
Checksum:i9725AC4F4240C985
GO

Sequence cautioni

The sequence CCE69486.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248283 Genomic DNA. Translation: CAB49034.1.
HE613800 Genomic DNA. Translation: CCE69486.1. Different initiation.
PIRiC75198.
RefSeqiNP_125803.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB49034; CAB49034; PAB0068.
GeneIDi1494997.
KEGGipab:PAB0068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248283 Genomic DNA. Translation: CAB49034.1 .
HE613800 Genomic DNA. Translation: CCE69486.1 . Different initiation.
PIRi C75198.
RefSeqi NP_125803.1. NC_000868.1.

3D structure databases

ProteinModelPortali Q9V2G4.
SMRi Q9V2G4. Positions 5-61, 524-668, 1246-1436.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9V2G4. 1 interaction.
MINTi MINT-7906090.
STRINGi 272844.PAB0068.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB49034 ; CAB49034 ; PAB0068 .
GeneIDi 1494997.
KEGGi pab:PAB0068.

Phylogenomic databases

eggNOGi COG1372.
HOGENOMi HOG000154102.
KOi K04801.
OMAi TNDENEM.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
1.10.260.40. 1 hit.
2.170.16.10. 6 hits.
3.10.28.10. 2 hits.
3.40.50.300. 3 hits.
InterProi IPR003959. ATPase_AAA_core.
IPR001387. Cro/C1-type_HTH.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
PF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 2 hits.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00530. HTH_XRE. 1 hit.
[Graphical view ]
SUPFAMi SSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 5 hits.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 2 hits.
TIGRFAMsi TIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEi PS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.
  3. "Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain."
    Henneke G., Gueguen Y., Flament D., Azam P., Querellou J., Dietrich J., Huebscher U., Raffin J.-P.
    J. Mol. Biol. 323:795-810(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10, FUNCTION, SUBUNIT.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiRFCS_PYRAB
AccessioniPrimary (citable) accession number: Q9V2G4
Secondary accession number(s): G8ZFU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the potential ATP-binding site.

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3