SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9V2F4

- DP2L_PYRAB

UniProt

Q9V2F4 - DP2L_PYRAB

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA polymerase II large subunit

Gene
polC, PYRAB01200, PAB2404
Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation
Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.UniRule annotation

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA-directed DNA polymerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA catabolic process, exonucleolytic Source: UniProtKB-HAMAP
  2. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Protein family/group databases

MEROPSiN10.005.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase II large subunit (EC:2.7.7.7)
Short name:
Pol II
Cleaved into the following chain:
Alternative name(s):
Pab pol II intein
Gene namesi
Name:polC
Ordered Locus Names:PYRAB01200
ORF Names:PAB2404
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000810: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 954954DNA polymerase II large subunit, 1st part Reviewed predictionPRO_0000007304Add
BLAST
Chaini955 – 1139185Pab polC intein Reviewed predictionPRO_0000007305Add
BLAST
Chaini1140 – 1455316DNA polymerase II large subunit, 2nd part Reviewed predictionPRO_0000007306Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.UniRule annotation

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Heterodimer of a large subunit and a small subunit By similarity.

Protein-protein interaction databases

STRINGi272844.PAB2404.

Structurei

Secondary structure

1
1455
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi960 – 9667
Beta strandi969 – 9746
Helixi975 – 9784
Helixi979 – 9813
Beta strandi985 – 9884
Beta strandi991 – 9944
Beta strandi996 – 9994
Beta strandi1001 – 10077
Turni1008 – 10114
Beta strandi1012 – 102514
Beta strandi1030 – 10356
Beta strandi1040 – 10434
Beta strandi1045 – 10539
Beta strandi1056 – 10616
Helixi1062 – 10643
Beta strandi1070 – 10734
Beta strandi1083 – 10864
Beta strandi1103 – 11119
Beta strandi1114 – 11196
Beta strandi1126 – 11316
Turni1132 – 11343
Beta strandi1135 – 11384

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LCJNMR-A955-1139[»]
ProteinModelPortaliQ9V2F4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1933.
HOGENOMiHOG000286435.
KOiK02322.
OMAiRNCDGDE.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
HAMAPiMF_00324. DNApol_II_L_arch.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
PfamiPF03833. PolC_DP2. 1 hit.
[Graphical view]
SMARTiSM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9V2F4-1 [UniParc]FASTAAdd to Basket

« Hide

MELPKEMEEY FEMLQREIDK AYEIAKKARA QGKDPSLDVE IPQATDMAGR     50
VESLVGPPGV AKRIRELVKE YGKEIAALKI VDEIIEGKFG DLGSREKYAE 100
QAVRTALAIL TEGIVSAPIE GIANVKIKRN TWADNSEYLA LYYAGPIRSS 150
GGTAQALSVL VGDYVRRKLG LDRFKPSEKH IERMVEEVDL YHRAVTRLQY 200
HPSPEEVRLA MRNIPIEITG EATDDVEVSH RDVPGVETNQ LRGGAILVLA 250
EGVLQKAKKL VKYIDKMGIE GWEWLKEFVE AKEKGEPKEE GKEESLAEST 300
LEETKVEVDM GFYYSLYQKF KEEIAPSDKY AKEVIGGRPL FSDPSKPGGF 350
RLRYGRSRAS GFATWGINPA TMILVDEFLA IGTQLKTERP GKGAVVTPVT 400
TIEGPIVKLK DGSVLRVDDY NLALKVREDV EEILYLGDAV IAFGDFVENN 450
QTLLPANYCE EWWILEFVKA LKEIYEVHLE PFTENEEESI EEASDYLEID 500
PEFLKEMLRD PLRVKPPVEL AIHFSEVLGI PLHPYYTLYW NSVEPKDVEK 550
LWRLLKNYAE IEWSNFRGIK FAKKIVISQE KLGDSKRTLE LLGLPHTVRD 600
GNVIVDYPWA AALLTPLGNL NWEFMAKPLY ATIDIINENN EIKLRDRGIS 650
WIGARMGRPE KAKERKMKPP VQVLFPIGLA GGSSRDIKKA AEEGKVAEVE 700
IAFFKCPKCG HVGPEHLCPN CGTRKELLWV CPRCNAEYPE SQAEGYNYTC 750
PKCNVKLRPY AKRKIRPSEL LNRAMENVKV YGVDKLKGVM GMTSGWKMPE 800
PLEKGLLRAK NDVYVFKDGT IRFDATDAPI THFRPREIGV SVEKLRELGY 850
THDFEGKPLV SEDQIVELKP QDIILSKEAG RYLLKVAKFV DDLLEKFYGL 900
PRFYNAEKME DLIGHLVIGL APHTSAGIVG RIIGFVDALV GYAHPYFHAA 950
KRRNCFPGDT RILVQIDGVP QKITLRELYE LFEDERYENM VYVRKKPKRE 1000
IKVYSIDLET GKVVLTDIED VIKAPATDHL IRFELEDGRS FETTVDHPVL 1050
VYENGRFIEK RAFEVKEGDK VLVSELELVE QSSSSQDNPK NENLGSPEHD 1100
QLLEIKNIKY VRANDDFVFS LNAKKYHNVI INENIVTHQC DGDEDAVMLL 1150
LDALLNFSRY YLPEKRGGKM DAPLVITTRL DPREVDSEVH NMDIVRYYPL 1200
EFYEATYELK SPKELVGVIE RVEDRLGKPE MYYGLKFTHD TDDIALGPKM 1250
SLYKQLGDME EKVRRQLEVA KRIRAVDEHG VAEKILNSHL IPDLRGNLRS 1300
FTRQEFRCVK CNTKFRRPPL NGKCPVCGGK IVLTVSKGAI EKYLGTAKML 1350
VTEYNVKNYT RQRICLTERD IDSLFENVFP ETQLTLIVNP NDICQRLVMA 1400
RTGEVNKSGL LENLSNGSKK TEKAEKAEKP RKKSDEKPKK KRVISLEEFF 1450
SRKSK 1455
Length:1,455
Mass (Da):165,711
Last modified:May 1, 2000 - v1
Checksum:iBB092E7BB2FE0D21
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ248283 Genomic DNA. Translation: CAB49044.1.
HE613800 Genomic DNA. Translation: CCE69496.1.
PIRiE75199.
RefSeqiNP_125813.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB49044; CAB49044; PAB2404.
GeneIDi1495007.
KEGGipab:PAB2404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ248283 Genomic DNA. Translation: CAB49044.1 .
HE613800 Genomic DNA. Translation: CCE69496.1 .
PIRi E75199.
RefSeqi NP_125813.1. NC_000868.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LCJ NMR - A 955-1139 [» ]
ProteinModelPortali Q9V2F4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272844.PAB2404.

Protein family/group databases

MEROPSi N10.005.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB49044 ; CAB49044 ; PAB2404 .
GeneIDi 1495007.
KEGGi pab:PAB2404.

Phylogenomic databases

eggNOGi COG1933.
HOGENOMi HOG000286435.
KOi K02322.
OMAi RNCDGDE.

Family and domain databases

Gene3Di 2.170.16.10. 1 hit.
HAMAPi MF_00324. DNApol_II_L_arch.
InterProi IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view ]
Pfami PF03833. PolC_DP2. 1 hit.
[Graphical view ]
SMARTi SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 1 hit.
TIGRFAMsi TIGR01445. intein_Nterm. 1 hit.
TIGR00354. polC. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiDP2L_PYRAB
AccessioniPrimary (citable) accession number: Q9V2F4
Secondary accession number(s): G8ZFV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi