Q9V2F3 (DP2S_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II small subunit
Short name=Pol II
EC=2.7.7.7

Gene names

Name:	polB
Ordered Locus Names:	PYRAB01210
ORF Names:	PAB2266

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00325
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00325 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00325
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the DNA polymerase delta/II small subunit family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	DNA polymerase II small subunit HAMAP MF_00325		PRO_0000096179

Sequences

	Sequence		Length	Mass (Da)
	Q9V2F3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F556800288784814	FASTA	619	69,395
	10 20 30 40 50 60 MDELVKALER AGYLLTPSAY YLLVDHFKEG KFSLVELVKF AKSKGVFIID GDLAYEFLQF 70 80 90 100 110 120 LGLGVPQEIK ESYISTGEEA EKTVESQETR ASELEEGGVS QVSSGELQEL KEESPEISTT 130 140 150 160 170 180 EEEIGGLELV QSSISTGSEV EYNNGENGES VVVLDKYGYP ILYAPEEIGE EKEYSKYEDV 190 200 210 220 230 240 VIEWNPSVTP VQIEKNYEVK FDVRQVKLRP PKVKNGSGKE GEIIVEAYAS LFKSRLSKLK 250 260 270 280 290 300 RILRENPEIS NVVDIGKLNY VSGDEEVTII GLVNSKRETN RGLIFEVEDK TGIVKVFLPK 310 320 330 340 350 360 DSEDYREAFK VLPDAVVAFK GFYSKKGIFF ANKFYLPDVP LYRKQKPPLE EKVYAILISD 370 380 390 400 410 420 IHVGSREFCE KAFLKFLEWL NGHVESKEEE EIVSRVKYLI IAGDVVDGIG IYPGQYSDLV 430 440 450 460 470 480 IPDIFDQYEA LANLLANVPE HITMFIGPGN HDAARPAIPQ PEFYKEYAKP IYKLKNAIII 490 500 510 520 530 540 SNPAVIRLHG RDFLIAHGRG IEDVVSFVPG LTHHKPGLPM VELLKMRHLA PTFGGKVPIA 550 560 570 580 590 600 PDPEDLLVIE EVPDLVQMGH VHVYDAVVYR GVQLVNSATW QAQTEFQKMV NIVPTPAKVP 610 VVDVESARVV KVLDFSGWC

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248283 Genomic DNA. Translation: CAB49045.1.
PIR	F75199.
RefSeq	NP_125814.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V2F3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000003411; EBPYRP00000003342; EBPYRG00000003411.
GeneID	1495008.
GenomeReviews	Gene locus PYRAB01210 in contig AL096836_GR.
KEGG	pab:PAB2266.
NMPDR	fig|272844.1.peg.132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022734.
HOGENOM	HBG726778.
OMA	IPDIFDQ.
ProtClustDB	PRK04036.
Enzyme and pathway databases
BioCyc	PABY272844:PAB2266-MONOMER.
Family and domain databases
HAMAP	MF_00325. DNApol_II_A_arch. [Tree]
InterPro	IPR007185. DNA_pol_alpha/epsilon_bsu. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. IPR011149. Pol2_small_arc. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K02323.
Pfam	PF04042. DNA_pol_E_B. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PIRSF	PIRSF000803. Arc_Pol2_small. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2S_PYRAB

Accession

Primary (citable) accession number: Q9V2F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program