Q9V2A9 (NADE_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	PYRAB01650
ORF Names:	PAB2244

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Probable NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152229
Regions
	Nucleotide binding	28 – 35	8	ATP By similarity
Sites
	Active site	30	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V2A9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 162A4DBCC8BAFEC0	FASTA	257	28,959
	10 20 30 40 50 60 MRTLDYEKAI ERIIDFIREK CSNGVVVGIS GGVDSATVTY LATKALGREK VLGLIMPYYE 70 80 90 100 110 120 NQDVEDAKLV VESLGIEYRV INIRPIVDTI VSEIGIELDK KSLGNVMART RMMILYAHAN 130 140 150 160 170 180 SMNRMVLGTS NRSEFLTGYF TKWGDGASDY APLINLYKTE VWEIAKRIGV PERIVKKKPT 190 200 210 220 230 240 AGLWEGQTDE DELGISYKLL DEILWRLVDL KMEKEKIAED LGIPIEKVEH VENLVKGSEH 250 KRRLPIGPSF DDLIAGP

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248283 Genomic DNA. Translation: CAB49089.1.
PIR	B75205.
RefSeq	NP_125858.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V2A9.
SMR	Q9V2A9. Positions 1-256.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000003892; EBPYRP00000003823; EBPYRG00000003892.
GeneID	1495052.
GenomeReviews	Gene locus PYRAB01650 in contig AL096836_GR.
KEGG	pab:PAB2244.
NMPDR	fig|272844.1.peg.176.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022961.
HOGENOM	HBG351567.
OMA	DGAVDCH.
PhylomeDB	Q9V2A9.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	PABY272844:PAB2244-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_PYRAB

Accession

Primary (citable) accession number: Q9V2A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program