Q9V298 (AMYA_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase
EC=3.2.1.1

Gene names

Name:	amyA
Ordered Locus Names:	PYRAB01760
ORF Names:	PAB0118

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Sequence similarities	Belongs to the glycosyl hydrolase 57 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC alpha-mannosidase activity Inferred from electronic annotation. Source: InterPro carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 655	655	Alpha-amylase		PRO_0000184572
Sites
	Active site	123	1	Nucleophile By similarity
	Active site	214	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V298 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7F6F920B1A00EECE	FASTA	655	77,296
	10 20 30 40 50 60 MPRINFIFGI HNHQPLGNFG WVMEEAYEKA YRPFLEILEE YPNMKVAIHI SGILVEWLEE 70 80 90 100 110 120 NKPDYIDLLK SLVRKGQVEI VVAGFYEPVL AAIPKEDRLE QIYLLKEWAK KIGYDAKGLW 130 140 150 160 170 180 LTERVWQPEL VKTLREAGIE YVVVDDYHFM SAGLSKDQLF WPYYTEDGGE VITVFPIDEK 190 200 210 220 230 240 LRYLIPFRPV DKVISYLHSL ASEDESKVAV FHDDGEKFGI WPMTYEWVYE KGWLREFFDR 250 260 270 280 290 300 VSSDEAINIM LYSEYLQKFK PKGLVYLPIA SYFEMSEWSL PAQQAKLFVE FVEKLKELNM 310 320 330 340 350 360 FERYRVFVRG GIWKNFFYKY PEANYMHKRM LMLSRLLRDN PSARRFVLRA QCNDAYWHGV 370 380 390 400 410 420 FGGIYLPHLR RAIWSNLIKA HSHLEPKNKI LDVDFDGREE VFLENDNFII VVKPHYGGSI 430 440 450 460 470 480 FELSSKRRAV NYADVIARRW EHYHNLGESE SDDNENQEGV SSIHEIGKRI PEDIRKELAY 490 500 510 520 530 540 DRYRRGILQD HFFSANETLD RYRLAKYWEL GDFIEGVYNY EVGNGLVLWR RGKVLNVTVD 550 560 570 580 590 600 VKKSIEVRED GFSVRYTVLS EEDIEALFGI ELNIAVHSIK ESPEELIGKR IKVNDKYGVG 610 620 630 640 650 EFEIVLNKKA RIWKYPVKTL SQSEAGWDYI QQGVSYTIIY PINGRLDIEL YFREL

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248283 Genomic DNA. Translation: CAB49100.1.
PIR	E75206.
RefSeq	NP_125869.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V298.
SMR	Q9V298. Positions 3-655.
ModBase	Search...
Protein family/group databases
CAZy	GH57. Glycoside Hydrolase Family 57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000003382; EBPYRP00000003313; EBPYRG00000003382.
GeneID	1495063.
GenomeReviews	Gene locus PYRAB01760 in contig AL096836_GR.
KEGG	pab:PAB0118.
NMPDR	fig|272844.1.peg.187.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000023113.
HOGENOM	HBG460637.
OMA	LTERVWE.
ProtClustDB	CLSK2459743.
Enzyme and pathway databases
BioCyc	PABY272844:PAB0118-MONOMER.
Family and domain databases
InterPro	IPR015179. A-amylase/a-glucTrfase_C. IPR015178. A-amylase/a-glucTrfase_central. IPR011013. Glyco_hydro-type_carb-bd. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR000602. Glyco_hydro_38_core. IPR004300. Glyco_hydro_57_N. [Graphical view]
Gene3D	G3DSA:3.20.110.10. Glyco_hydro_38_core. 1 hit.
KO	K07405.
Pfam	PF09094. DUF1925. 1 hit. PF09095. DUF1926. 1 hit. PF03065. Glyco_hydro_57. 1 hit. [Graphical view]
ProDom	PD014405. DUF1925. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMYA_PYRAB

Accession

Primary (citable) accession number: Q9V298

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program