Reviewed, UniProtKB/Swiss-Prot Q9V298 (AMYA_PYRAB)

Names and origin

Protein names

Recommended name:
    Alpha-amylase
    EC=3.2.1.1

Gene names

Name:	amyA
Ordered Locus Names:	PYRAB01760
ORF Names:	PAB0118

Organism

Pyrococcus abyssi [Complete proteome] [HAMAP]

Taxonomic identifier

29292 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.
Sequence similarities	Belongs to the glycosyl hydrolase 57 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 655	655	Alpha-amylase		PRO_0000184572
Sites
	Active site	123	1	Nucleophile By similarity
	Active site	214	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V298-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7F6F920B1A00EECE	FASTA	655	77,296
	10 20 30 40 50 60 MPRINFIFGI HNHQPLGNFG WVMEEAYEKA YRPFLEILEE YPNMKVAIHI SGILVEWLEE 70 80 90 100 110 120 NKPDYIDLLK SLVRKGQVEI VVAGFYEPVL AAIPKEDRLE QIYLLKEWAK KIGYDAKGLW 130 140 150 160 170 180 LTERVWQPEL VKTLREAGIE YVVVDDYHFM SAGLSKDQLF WPYYTEDGGE VITVFPIDEK 190 200 210 220 230 240 LRYLIPFRPV DKVISYLHSL ASEDESKVAV FHDDGEKFGI WPMTYEWVYE KGWLREFFDR 250 260 270 280 290 300 VSSDEAINIM LYSEYLQKFK PKGLVYLPIA SYFEMSEWSL PAQQAKLFVE FVEKLKELNM 310 320 330 340 350 360 FERYRVFVRG GIWKNFFYKY PEANYMHKRM LMLSRLLRDN PSARRFVLRA QCNDAYWHGV 370 380 390 400 410 420 FGGIYLPHLR RAIWSNLIKA HSHLEPKNKI LDVDFDGREE VFLENDNFII VVKPHYGGSI 430 440 450 460 470 480 FELSSKRRAV NYADVIARRW EHYHNLGESE SDDNENQEGV SSIHEIGKRI PEDIRKELAY 490 500 510 520 530 540 DRYRRGILQD HFFSANETLD RYRLAKYWEL GDFIEGVYNY EVGNGLVLWR RGKVLNVTVD 550 560 570 580 590 600 VKKSIEVRED GFSVRYTVLS EEDIEALFGI ELNIAVHSIK ESPEELIGKR IKVNDKYGVG 610 620 630 640 650 EFEIVLNKKA RIWKYPVKTL SQSEAGWDYI QQGVSYTIIY PINGRLDIEL YFREL

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ248283 Genomic DNA. Translation: CAB49100.1.
PIR	E75206.
RefSeq	NP_125869.1.
3D structure databases
HSSP	HSSP built from PDB template 1K1W based on UniProtKB O32462.
ModBase	Search...
Protein family/group databases
CAZy	GH57. Glycoside Hydrolase Family 57.
Genome annotation databases
GeneID	1495063.
GenomeReviews	Gene locus PYRAB01760 in contig AL096836_GR.
KEGG	pab:PAB0118.
NMPDR	fig|272844.1.peg.187.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9V298.
OMA	Q9V298. WLTERVW.
Enzyme and pathway databases
BioCyc	PABY272844:PAB0118-MON.
BRENDA	3.2.1.1. 262861.
Family and domain databases
InterPro	IPR015179. a-amylase/a-glucanoTrfase_prok. IPR015178. DUF1925. IPR004300. Glyco_hydro_57_core. [Graphical view]
Pfam	PF09094. DUF1925. 1 hit. PF09095. DUF1926. 1 hit. PF03065. Glyco_hydro_57. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYA_PYRAB

Accession

Primary (citable) accession number: Q9V298

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)