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Q9V298 (AMYA_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-amylase

EC=3.2.1.1
Gene names
Name:amyA
Ordered Locus Names:PYRAB01760
ORF Names:PAB0118
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length655 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Sequence similarities

Belongs to the glycosyl hydrolase 57 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionalpha-amylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 655655Alpha-amylase
PRO_0000184572

Sites

Active site1231Nucleophile By similarity
Active site2141Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9V298 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 7F6F920B1A00EECE

FASTA65577,296
        10         20         30         40         50         60 
MPRINFIFGI HNHQPLGNFG WVMEEAYEKA YRPFLEILEE YPNMKVAIHI SGILVEWLEE 

        70         80         90        100        110        120 
NKPDYIDLLK SLVRKGQVEI VVAGFYEPVL AAIPKEDRLE QIYLLKEWAK KIGYDAKGLW 

       130        140        150        160        170        180 
LTERVWQPEL VKTLREAGIE YVVVDDYHFM SAGLSKDQLF WPYYTEDGGE VITVFPIDEK 

       190        200        210        220        230        240 
LRYLIPFRPV DKVISYLHSL ASEDESKVAV FHDDGEKFGI WPMTYEWVYE KGWLREFFDR 

       250        260        270        280        290        300 
VSSDEAINIM LYSEYLQKFK PKGLVYLPIA SYFEMSEWSL PAQQAKLFVE FVEKLKELNM 

       310        320        330        340        350        360 
FERYRVFVRG GIWKNFFYKY PEANYMHKRM LMLSRLLRDN PSARRFVLRA QCNDAYWHGV 

       370        380        390        400        410        420 
FGGIYLPHLR RAIWSNLIKA HSHLEPKNKI LDVDFDGREE VFLENDNFII VVKPHYGGSI 

       430        440        450        460        470        480 
FELSSKRRAV NYADVIARRW EHYHNLGESE SDDNENQEGV SSIHEIGKRI PEDIRKELAY 

       490        500        510        520        530        540 
DRYRRGILQD HFFSANETLD RYRLAKYWEL GDFIEGVYNY EVGNGLVLWR RGKVLNVTVD 

       550        560        570        580        590        600 
VKKSIEVRED GFSVRYTVLS EEDIEALFGI ELNIAVHSIK ESPEELIGKR IKVNDKYGVG 

       610        620        630        640        650 
EFEIVLNKKA RIWKYPVKTL SQSEAGWDYI QQGVSYTIIY PINGRLDIEL YFREL 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248283 Genomic DNA. Translation: CAB49100.1.
HE613800 Genomic DNA. Translation: CCE69552.1.
PIRE75206.
RefSeqNP_125869.1. NC_000868.1.

3D structure databases

ProteinModelPortalQ9V298.
SMRQ9V298. Positions 3-655.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272844.PAB0118.

Protein family/group databases

CAZyGH57. Glycoside Hydrolase Family 57.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB49100; CAB49100; PAB0118.
GeneID1495063.
KEGGpab:PAB0118.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1449.
HOGENOMHOG000223207.
KOK07405.
OMAHFMSAGL.
ProtClustDBCLSK2459743.

Family and domain databases

Gene3D2.70.98.10. 1 hit.
3.20.110.10. 2 hits.
InterProIPR015179. A-amylase/a-glucTrfase_C.
IPR015178. A-amylase/a-glucTrfase_central.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR004300. Glyco_hydro_57_N.
[Graphical view]
PfamPF09094. DUF1925. 1 hit.
PF09095. DUF1926. 1 hit.
PF03065. Glyco_hydro_57. 1 hit.
[Graphical view]
ProDomPD014405. DUF1925. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF74650. SSF74650. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAMYA_PYRAB
AccessionPrimary (citable) accession number: Q9V298
Secondary accession number(s): G8ZG11
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: October 16, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries