Q9V290 (PRNK_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyribonucleotide 5'-hydroxyl-kinase PYRAB01840
EC=2.7.1.78

Alternative name(s):
Polynucleotide kinase PYRAB01840

Gene names

Ordered Locus Names:

PYRAB01840

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA By similarity.
Catalytic activity	ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA. ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.
Cofactor	Divalent cation By similarity.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Polyribonucleotide 5'-hydroxyl-kinase PYRAB01840		PRO_0000376015
Regions
	Nucleotide binding	39 – 46	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9V290 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 5667B23E9A559DEE	FASTA	357	40,410
	10 20 30 40 50 60 MMRMASNKAM FTNDVPQDRL LAVEKIESLR KPAKVMIIGD VDTGKTTLTI YLANELLSRG 70 80 90 100 110 120 FRVAIIDSDI GQKGILPPAT ISLAFVDSHF TSLDDLKAFS HYFIGTITPN QYFGEMVVGV 130 140 150 160 170 180 MKLSELAMKF SDVVLIDTTG MIYGSGVELK RMKIEAVKPN LILALERNNE LAPILKGYED 190 200 210 220 230 240 ITIRLEVSEK AKDFSRSERR ELRREKWRKY FENSKIVNFN LDDVLVTGTS LFQGEEIGDT 250 260 270 280 290 300 EKSLLERLFK WLIIHGRKIG NKYFVVKVDA SEGPRIVDKN VVKYFDFSKL SNILLGLIDK 310 320 330 340 350 QGFCIGLGIL KSINFKEKKI EVLTPVEDLS SVAEIRFGRV RVREDGEELG LLDREAL

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248283 Genomic DNA. Translation: CAB49108.1.
PIR	E75207.
RefSeq	NP_125877.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V290.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002312; EBPYRP00000002243; EBPYRG00000002312.
GeneID	1495071.
GenomeReviews	Gene locus PYRAB01840 in contig AL096836_GR.
NMPDR	fig|272844.1.peg.195.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000023291.
HOGENOM	HBG538965.
OMA	NDLGPPT.
ProtClustDB	CLSK544691.
Enzyme and pathway databases
BioCyc	PABY272844:PAB0124-MONOMER.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PRNK_PYRAB

Accession

Primary (citable) accession number: Q9V290

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 1, 2000
Last modified:	October 19, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program