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Q9V253 (PUR1_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:PYRAB02210
ORF Names:PAB0151
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 1010 Probable
PRO_0000029277
Chain11 – 447437Amidophosphoribosyltransferase
PRO_0000029278

Regions

Domain11 – 224214Glutamine amidotransferase type-2

Sites

Active site111For GATase activity By similarity
Metal binding2391Iron-sulfur (4Fe-4S) By similarity
Metal binding2861Magnesium By similarity
Metal binding3461Magnesium By similarity
Metal binding3471Magnesium By similarity
Metal binding3831Iron-sulfur (4Fe-4S) By similarity
Metal binding4321Iron-sulfur (4Fe-4S) By similarity
Metal binding4351Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9V253 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 94E0B221A8B3F5DF

FASTA44749,772
        10         20         30         40         50         60 
MAKVRGMREK CGIFGVSSED AVRKTYYGLI ALQHRGQEGA GISVWNGRIR TIKGHGLVSE 

        70         80         90        100        110        120 
VFDENSLNLA SNIAIGHVRY STSGSLSEVQ PLEVRCCGYE LAIAHNGTLT NFIPLRRLYE 

       130        140        150        160        170        180 
GMGIKFHSSV DTEVIGISFL NHYSQVKDEF EAMRRVFEEV KGAYSILILF DGKIIAARDP 

       190        200        210        220        230        240 
VGFRPLVFGE GDGYYFASED SALRMFGLET RDVLPGEVFV IDGESYESKV LAREKHAYCV 

       250        260        270        280        290        300 
FEYIYFARPD STLNGINVYC ARYRMGVELA RESPAEGDVV IAVPDSGRTA ALGFAHESGI 

       310        320        330        340        350        360 
PYMEGLIKNR YIGRTFIMPS GRGLKVKLKL SPVREVVNGK RVVLVDDSIV RGTTMTRIVK 

       370        380        390        400        410        420 
MLRDAGAREV HVRIASPPIR YPCYMGIDIP TRHELIAAWR SIEDIRKEIG ADSLAYLSIE 

       430        440 
GLKRAVGTKN LCMACLTGEY PEWAFNF 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248283 Genomic DNA. Translation: CAB49145.1.
HE613800 Genomic DNA. Translation: CCE69597.1.
PIRB75212.
RefSeqNP_125914.1. NC_000868.1.

3D structure databases

ProteinModelPortalQ9V253.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272844.PAB0151.

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB49145; CAB49145; PAB0151.
GeneID1495110.
KEGGpab:PAB0151.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033688.
KOK00764.
OMACDACFTG.
ProtClustDBPRK08341.

Enzyme and pathway databases

UniPathwayUPA00074; UER00124.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR000836. PRibTrfase_dom.
[Graphical view]
PfamPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_PYRAB
AccessionPrimary (citable) accession number: Q9V253
Secondary accession number(s): G8ZG56
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: May 1, 2000
Last modified: October 16, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways