Reviewed, UniProtKB/Swiss-Prot Q9V1R3 (HMDH_PYRAB)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:	hmgA
Ordered Locus Names:	PYRAB03640
ORF Names:	PAB2106

Organism

Pyrococcus abyssi [Complete proteome] [HAMAP]

Taxonomic identifier

29292 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts HMG-CoA to mevalonate By similarity.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114463
Sites
	Active site	104	1	Charge relay system By similarity
	Active site	310	1	Charge relay system By similarity
	Active site	406	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V1R3-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 070BA13A30631B31	FASTA	411	44,349
	10 20 30 40 50 60 MKTLNVEDII EKVANGEIKL HQVEKYVNGD KRLATEIRRK ALERKLGISL KHIGHYSIDP 70 80 90 100 110 120 NELIGRNIEN MIGVVQIPMG VAGPLKINGE YAKGEFYIPL ATTEGALVAS VNRGCSALTE 130 140 150 160 170 180 AGGVVTTILD DKMTRAPLIR CPNARRAREV AEWVKENLNY LQEKAVAKVT RHGKLRDVKP 190 200 210 220 230 240 FIVGNNLYLR FEFETGDAMG MNMVTIASEE IMKVIEEEFP DVRYLALSGN LCVDKKPNAV 250 260 270 280 290 300 NFILGRGKTV VAEAIVPREI VEKKLKTTPE LIAEVNYFKN LVGSAQAGSY GFNAHFGNIV 310 320 330 340 350 360 GAIFLATGQD EAQITEGSHG ITIAEVTPEG DLYISITMPS LEIGTVGGGT RVPTQREALS 370 380 390 400 410 IMGVAGGGDP PGVNAKKFAE IVAGAVLAGE LSLLAAIAAK HLARAHKMLG R

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ248284 Genomic DNA. Translation: CAB49286.1.
PIR	G75150.
RefSeq	NP_126055.1.
3D structure databases
HSSP	HSSP built from PDB template 1HWI based on UniProtKB P04035.
ModBase	Search...
Genome annotation databases
GeneID	1495254.
GenomeReviews	Gene locus PYRAB03640 in contig AL096836_GR.
KEGG	pab:PAB2106.
NMPDR	fig|272844.1.peg.378.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9V1R3.
OMA	AHFANII.
Enzyme and pathway databases
BioCyc	PABY272844:PAB2106-MON.
BRENDA	1.1.1.34. 262861.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. False negative. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_PYRAB

Accession

Primary (citable) accession number: Q9V1R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)