ogt

Functionⁱ

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.By similarity

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.By similarity

Catalytic activityⁱ

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.By similarity

DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	142	Nucleophile; methyl group acceptorBy similarity		1

GO - Molecular functionⁱ

methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA repair Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Biological process	DNA damage, DNA repair

Enzyme and pathway databases

BioCycⁱ	PABY272844:G1GT8-419-MONOMER.

BioCycⁱ

PABY272844:G1GT8-419-MONOMER.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Methylated-DNA--protein-cysteine methyltransferaseBy similarity (EC:2.1.1.63By similarity) Alternative name(s): 6-O-methylguanine-DNA methyltransferaseBy similarity Short name: MGMTBy similarity O-6-methylguanine-DNA-alkyltransferaseBy similarity
Gene namesⁱ	Name:ogtBy similarity Ordered Locus Names:PYRAB03900 ORF Names:PAB0260
Organismⁱ	Pyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifierⁱ	272844 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus abyssi
Proteomesⁱ	UP000000810 Componentⁱ: Chromosome UP000009139 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
By similarity
Manual assertion inferred from sequence similarity toⁱ
- UniProtKB:O74023 (OGT_THEKO)

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000139381	1 – 172	Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST		172

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	272844.PAB0260.

STRINGⁱ

272844.PAB0260.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9V1N7.
SMRⁱ	Q9V1N7.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the MGMT family.By similarity

Phylogenomic databases

eggNOGⁱ	arCOG02724. Archaea. COG0350. LUCA.
HOGENOMⁱ	HOG000244137.
KEGG Orthology (KO) More...KOⁱ	K00567.
OMAⁱ	KRNPYPI.
Database of Orthologous Groups More...OrthoDBⁱ	POG093Z0E1E.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06445. ATase. 1 hit.
Gene3Dⁱ	1.10.10.10. 1 hit.
InterProⁱ	View protein in InterPro IPR015236. DUF1938. IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA-bd. IPR036217. MethylDNA_cys_MeTrfase_DNAb. IPR036631. MGMT_N_sf. IPR036388. WH-like_DNA-bd_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01035. DNA_binding_1. 1 hit. PF09153. DUF1938. 1 hit.
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD068053. DUF1938. 1 hit.
SUPFAMⁱ	SSF46767. SSF46767. 1 hit. SSF53155. SSF53155. 1 hit.
TIGRFAMsⁱ	TIGR00589. ogt. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00374. MGMT. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9V1N7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLSCESFKIK GREIIICVIW EEGIQGIVYS LDGREFLEKQ LSRLISMLNK 
        60         70         80         90        100
RGVSLSLKER HSKYPELVFN VLTGKISNEE GFEELSLEGL TDFEIRVYSW 
       110        120        130        140        150
LVKNVKRGEV ITYGKVAKEL KTSALAIGGA MKRNPYPIVV PCHRVVGKKD 
       160        170 
PWLYTPKPEY KKFLLEVEGW TS

Length:172

Mass (Da):19,713

Last modified:May 1, 2000 - v1

Checksum:ⁱBEE0E6A7B436384E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ248284 Genomic DNA. Translation: CAB49312.1. HE613800 Genomic DNA. Translation: CCE69768.1.
PIRⁱ	A75154.
NCBI Reference Sequences More...RefSeqⁱ	WP_010867512.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaⁱ	CAB49312; CAB49312; PAB0260.
GeneIDⁱ	1495281.
KEGGⁱ	pab:PAB0260.
PATRICⁱ	fig\|272844.11.peg.411.

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9V1N7	O74023 A0A142CUV1 UPI000B59B225 O59499 C6A382 A0A101ENI5 A0A101F0B7 A0A0S1XDT5 UPI000704886C F0LIT8 +27	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) Thermococcus peptonophilus Thermococcus gorgonarius Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) Thermococcus sibiricus (strain MM 739 / DSM 12597) Thermococcus sibiricus Thermococcus sp. 40_45 Thermococcus barophilus Thermococcus barophilus (strain DSM 11836 / MP) Thermococcus eurythermalis And more	174	UniRef50_O74023	Cluster: Methylated-DNA--protein-cysteine methyltransferase	38

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ248284 Genomic DNA. Translation: CAB49312.1. HE613800 Genomic DNA. Translation: CCE69768.1.
PIRⁱ	A75154.
NCBI Reference Sequences More...RefSeqⁱ	WP_010867512.1. NC_000868.1.

3D structure databases

ProteinModelPortalⁱ	Q9V1N7.
SMRⁱ	Q9V1N7.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	272844.PAB0260.

STRINGⁱ

272844.PAB0260.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	CAB49312; CAB49312; PAB0260.
GeneIDⁱ	1495281.
KEGGⁱ	pab:PAB0260.
PATRICⁱ	fig\|272844.11.peg.411.

Phylogenomic databases

eggNOGⁱ	arCOG02724. Archaea. COG0350. LUCA.
HOGENOMⁱ	HOG000244137.
KEGG Orthology (KO) More...KOⁱ	K00567.
OMAⁱ	KRNPYPI.
Database of Orthologous Groups More...OrthoDBⁱ	POG093Z0E1E.

Enzyme and pathway databases

BioCycⁱ	PABY272844:G1GT8-419-MONOMER.

BioCycⁱ

PABY272844:G1GT8-419-MONOMER.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06445. ATase. 1 hit.
Gene3Dⁱ	1.10.10.10. 1 hit.
InterProⁱ	View protein in InterPro IPR015236. DUF1938. IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA-bd. IPR036217. MethylDNA_cys_MeTrfase_DNAb. IPR036631. MGMT_N_sf. IPR036388. WH-like_DNA-bd_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01035. DNA_binding_1. 1 hit. PF09153. DUF1938. 1 hit.
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD068053. DUF1938. 1 hit.
SUPFAMⁱ	SSF46767. SSF46767. 1 hit. SSF53155. SSF53155. 1 hit.
TIGRFAMsⁱ	TIGR00589. ogt. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00374. MGMT. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	OGT_PYRAB
Accessionⁱ	Q9V1N7Primary (citable) accession number: Q9V1N7 Secondary accession number(s): G8ZI25
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
	Last sequence update:	May 1, 2000
	Last modified:	February 28, 2018
	This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9V1N7 (OGT_PYRAB)

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Methylated-DNA--protein-cysteine methyltransferase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

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Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ