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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.By similarity

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.By similarity

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.By similarity
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei142Nucleophile; methyl group acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciPABY272844:G1GT8-419-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseBy similarity (EC:2.1.1.63By similarity)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseBy similarity
Short name:
MGMTBy similarity
O-6-methylguanine-DNA-alkyltransferaseBy similarity
Gene namesi
Name:ogtBy similarity
Ordered Locus Names:PYRAB03900
ORF Names:PAB0260
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393811 – 172Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST172

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB0260.

Structurei

3D structure databases

ProteinModelPortaliQ9V1N7.
SMRiQ9V1N7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.By similarity

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
HOGENOMiHOG000244137.
KOiK00567.
OMAiKRNPYPI.
OrthoDBiPOG093Z0E1E.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR015236. DUF1938.
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR036217. MethylDNA_cys_MeTrfase_DNAb.
IPR036631. MGMT_N_sf.
IPR036388. WH-like_DNA-bd_sf.
PfamiView protein in Pfam
PF01035. DNA_binding_1. 1 hit.
PF09153. DUF1938. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD068053. DUF1938. 1 hit.
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiView protein in PROSITE
PS00374. MGMT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9V1N7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSCESFKIK GREIIICVIW EEGIQGIVYS LDGREFLEKQ LSRLISMLNK
60 70 80 90 100
RGVSLSLKER HSKYPELVFN VLTGKISNEE GFEELSLEGL TDFEIRVYSW
110 120 130 140 150
LVKNVKRGEV ITYGKVAKEL KTSALAIGGA MKRNPYPIVV PCHRVVGKKD
160 170
PWLYTPKPEY KKFLLEVEGW TS
Length:172
Mass (Da):19,713
Last modified:May 1, 2000 - v1
Checksum:iBEE0E6A7B436384E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248284 Genomic DNA. Translation: CAB49312.1.
HE613800 Genomic DNA. Translation: CCE69768.1.
PIRiA75154.
RefSeqiWP_010867512.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB49312; CAB49312; PAB0260.
GeneIDi1495281.
KEGGipab:PAB0260.
PATRICifig|272844.11.peg.411.

Similar proteinsi

Entry informationi

Entry nameiOGT_PYRAB
AccessioniPrimary (citable) accession number: Q9V1N7
Secondary accession number(s): G8ZI25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 1, 2000
Last modified: February 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome