Q9V1I9 (LEUD2_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydratase small subunit 2
EC=4.2.1.33

Alternative name(s):
Alpha-IPM isomerase 2
Short name=IPMI 2
Isopropylmalate isomerase 2

Gene names

Name:	leuD2
Synonyms:	leuD-2
Ordered Locus Names:	PYRAB04380
ORF Names:	PAB0288

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. HAMAP MF_01032
Catalytic activity	(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H2O. HAMAP MF_01032 (2S)-2-isopropylmaleate + H2O = (2S)-2-isopropylmalate. HAMAP MF_01032
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP MF_01032
Subunit structure	Heterodimer of LeuC and LeuD By similarity. HAMAP MF_01032
Sequence similarities	Belongs to the LeuD family. LeuD type 2 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	3-isopropylmalate dehydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	3-isopropylmalate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 163	163	3-isopropylmalate dehydratase small subunit 2 HAMAP MF_01032		PRO_0000141945

Sequences

	Sequence		Length	Mass (Da)
	Q9V1I9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F759C5473A36B632	FASTA	163	18,010
	10 20 30 40 50 60 MITTGRVWKF WDNVSTDEIT PGRYNLTKDP QELARIAFIE VRPEFAEKVR RGDVVVGGKN 70 80 90 100 110 120 FGIGSSRESA ALALKAAGVS GIIAKSFGRI FYRNAVNLGI PLLIGDTDEL EDGDVITVNW 130 140 150 160 ETGEVRKNGQ TLQFEPLPGF LLEIVREGGI LEFIRRRGDL CIG

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248284 Genomic DNA. Translation: CAB49360.1.
PIR	A75160.
RefSeq	NP_126129.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V1I9.
SMR	Q9V1I9. Positions 1-162.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002332; EBPYRP00000002263; EBPYRG00000002332.
GeneID	1495332.
GenomeReviews	Gene locus PYRAB04380 in contig AL096836_GR.
KEGG	pab:PAB0288.
NMPDR	fig|272844.1.peg.456.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022453.
HOGENOM	HBG304838.
OMA	ITPGRYN.
PhylomeDB	Q9V1I9.
ProtClustDB	PRK00439.
Enzyme and pathway databases
BioCyc	PABY272844:PAB0288-MONOMER.
Family and domain databases
HAMAP	MF_01032. LeuD_type2. [Tree]
InterPro	IPR015937. Acoase/IPM_deHydtase. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. IPR011827. IsopropMal_deHydtase_ssu. [Graphical view]
Gene3D	G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit.
KO	K01704.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit.
Pfam	PF00694. Aconitase_C. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR02087. LEUD_arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEUD2_PYRAB

Accession

Primary (citable) accession number: Q9V1I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program