Q9V1I3 (LYSK_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-lysine deacetylase
EC=3.5.1.-

Gene names

Name:	lysK
Ordered Locus Names:	PYRAB04440
ORF Names:	PAB0294

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-lysine + H2O = acetate + L-lysine. HAMAP MF_01120
Cofactor	Binds 2 zinc or cobalt ions per subunit By similarity. HAMAP MF_01120 Glutathione By similarity. HAMAP MF_01120
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 5/5. HAMAP MF_01120
Subunit structure	Homodimer By similarity. HAMAP MF_01120
Subcellular location	Cytoplasm Probable HAMAP MF_01120.
Sequence similarities	Belongs to the peptidase M20A family. LysK subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Cobalt Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cobalt ion binding Inferred from electronic annotation. Source: InterPro hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Inferred from electronic annotation. Source: InterPro metallopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Acetyl-lysine deacetylase HAMAP MF_01120		PRO_0000185345
Sites
	Active site	69	1	By similarity
	Active site	118	1	Proton acceptor By similarity
	Metal binding	67	1	Cobalt or zinc 1 By similarity
	Metal binding	91	1	Cobalt or zinc 1 By similarity
	Metal binding	91	1	Cobalt or zinc 2 By similarity
	Metal binding	119	1	Cobalt or zinc 2 By similarity
	Metal binding	140	1	Cobalt or zinc 1 By similarity
	Metal binding	298	1	Cobalt or zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V1I3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B082B30C55C524CB	FASTA	337	37,448
	10 20 30 40 50 60 MEIPKDEKIK FLKELVEIYS PTGKEEEAAK FIKEKLEEYG VKAYIDKVGN VIGVKEGEGP 70 80 90 100 110 120 LILLAGHVDT VPGYIPVRIE GDILWGRGSV DAKGPLSALL FAMVESNANV IFAGLVDEEG 130 140 150 160 170 180 FSKGARALDV PRPEYVIVGE PSGVNGVTIG YKGSLTVRFV ERVEKFHGSI GGGAAEKLIE 190 200 210 220 230 240 RWLSISGNFE DGFNGLSGRI VRFVAYERDF EFYGEMIVNL RTPPGYEPPR DWDIIDFVPA 250 260 270 280 290 300 YEVNRRSPLV RTFVRSIREL GMKPKLKKKS GTADMNILAP RFGVDAVAYG PGDSRLDHTP 310 320 330 YERISLMEYL QSIDVLKNVL TKLKGKDLDK IYKSSPR

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248284 Genomic DNA. Translation: CAB49366.1.
PIR	G75160.
RefSeq	NP_126135.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V1I3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002959; EBPYRP00000002890; EBPYRG00000002959.
GeneID	1495339.
GenomeReviews	Gene locus PYRAB04440 in contig AL096836_GR.
KEGG	pab:PAB0294.
NMPDR	fig|272844.1.peg.463.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022564.
HOGENOM	HBG728841.
OMA	LDHTPYE.
ProtClustDB	PRK04443.
Enzyme and pathway databases
BioCyc	PABY272844:PAB0294-MONOMER.
Family and domain databases
HAMAP	MF_01120. Acet-lys_deacetyl. [Tree]
InterPro	IPR023556. Acetyl-lysine_deAcylase. IPR001261. ArgE/DapE_CS. IPR010175. DapE_lys_deAc. IPR002933. Peptidase_M20. [Graphical view]
KO	K05831.
Pfam	PF01546. Peptidase_M20. 1 hit. [Graphical view]
TIGRFAMs	TIGR01902. DapE-lys-deAc. 1 hit.
PROSITE	PS00758. ARGE_DAPE_CPG2_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSK_PYRAB

Accession

Primary (citable) accession number: Q9V1I3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program