Q9V1H1 (AROA_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	PYRAB04560
ORF Names:	PAB0306

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Probable 3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088334

Sequences

	Sequence		Length	Mass (Da)
	Q9V1H1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: FE2303174F5340C8	FASTA	410	44,464
	10 20 30 40 50 60 MFGPVSVEMI IERVDEVRGK VKAPPSKSYT HRAYFLSLLA DSPSKVMNPL ISEDTIASLD 70 80 90 100 110 120 AISKFGAQVN GNKIIPPQEL TPGKIDARES GTTARISLAV ASLARGTSVI TGKGRLVERP 130 140 150 160 170 180 FKPLVDALRS LKVKISGEKL PIAVEGGNPV GEYVKVDCSL SSQFGTAMLI LASKIGLTVE 190 200 210 220 230 240 MLNPVSRPYI EVTLKVMESF GIEFERNGFK VKVHPGIRGS KFHVPGDYSS ASFFLAAGAL 250 260 270 280 290 300 YGKVKVSNLV KDDPQADARI IDILEEFGAD VKVGRKYVVV ERNEMKPINV DCSNFPDLFP 310 320 330 340 350 360 ILAVLASYAE GKSVITGRQL RLKESDRVKA VAVNLRKAGI KVKELPNGLE IVGGKPRGFT 370 380 390 400 410 VESFNDHRIV MAMAILGLGA EGKTIIKDPH VVSKSYPSFF LDLRRVLNEG

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248284 Genomic DNA. Translation: CAB49378.1.
PIR	C75162.
RefSeq	NP_126147.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V1H1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002893; EBPYRP00000002824; EBPYRG00000002893.
GeneID	1495352.
GenomeReviews	Gene locus PYRAB04560 in contig AL096836_GR.
KEGG	pab:PAB0306.
NMPDR	fig|272844.1.peg.476.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000023163.
HOGENOM	HBG646626.
OMA	FADHRIA.
PhylomeDB	Q9V1H1.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	PABY272844:PAB0306-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. False negative. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_PYRAB

Accession

Primary (citable) accession number: Q9V1H1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program