Q9V1G9 (TRPA_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase alpha chain
EC=4.2.1.20

Gene names

Name:	trpA
Ordered Locus Names:	PYRAB04580
ORF Names:	PAB2049

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. HAMAP MF_00131
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP MF_00131
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP MF_00131
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpA family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	tryptophan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Tryptophan synthase alpha chain HAMAP MF_00131		PRO_0000098896
Sites
	Active site	36	1	Proton acceptor By similarity
	Active site	47	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V1G9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 400E27D2E17F7440	FASTA	248	27,288
	10 20 30 40 50 60 MFRDGSLIPY LTAGDPSAKA TLRFLLAIEE YSGAIELGIP FSDPIADGKT IQQSHFRALK 70 80 90 100 110 120 GGFKLEHAFN IVREFRKHSD VPIVLMTYYN PVFRVGLREF IGKAKDSGVD GMLIVDLPVM 130 140 150 160 170 180 HASEFLEVAR EEGIKTVFLA APNTPDERLK EIDKASTGFV YLISLYGTTG ARDKIPETAF 190 200 210 220 230 240 NLLKRAKRIC KNKVAVGFGV SKREHVEMLL NAGANGVVVG SALINIIAEH GENAEEKLRE KVRELAGL

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248284 Genomic DNA. Translation: CAB49380.1.
PIR	E75162.
RefSeq	NP_126149.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V1G9.
SMR	Q9V1G9. Positions 1-248.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002707; EBPYRP00000002638; EBPYRG00000002707.
GeneID	1495354.
GenomeReviews	Gene locus PYRAB04580 in contig AL096836_GR.
KEGG	pab:PAB2049.
NMPDR	fig|272844.1.peg.478.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022998.
HOGENOM	HBG391690.
OMA	NPIYIYG.
PhylomeDB	Q9V1G9.
ProtClustDB	PRK13111.
Enzyme and pathway databases
BioCyc	PABY272844:PAB2049-MONOMER.
Family and domain databases
HAMAP	MF_00131. Trp_synth_alpha. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR018204. Trp_synthase_alpha_AS. IPR002028. Trp_synthase_suA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01695.
Pfam	PF00290. Trp_syntA. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00262. TrpA. 1 hit.
PROSITE	PS00167. TRP_SYNTHASE_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPA_PYRAB

Accession

Primary (citable) accession number: Q9V1G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program