Q9V1E3 (SYE_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	PYRAB04840
ORF Names:	PAB0323

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus abyssi

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_0000119725
Regions
	Motif	106 – 116	11	"HIGH" region HAMAP-Rule MF_00022

Sequences

	Sequence		Length	Mass (Da)
	Q9V1E3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D7BA5D954B1A8AC6	FASTA	571	65,920
	10 20 30 40 50 60 MEVERIALKY ALINAIEHGG KANPKAVIGK VLGENPELRS KAREIVPIIN KVVEEVNSLS 70 80 90 100 110 120 LDEQKAKLME IYPEYFEKKE EKKEEKKGLP PLPKAEKGKV VTRFAPNPDG AFHLGNARAA 130 140 150 160 170 180 ILSYEYAKMY GGKFILRFDD TDPKVKRPEP IFYEMIIEDL EWLGIKPDEI VYASDRLELY 190 200 210 220 230 240 YKYAEELIKM GKAYVCTCKP EKFRELRDKG IPCPHRDEPV EVQLERWRKM LNGEYKEGEA 250 260 270 280 290 300 VVRIKTDLNH PNPAVRDWPA LRIVDNPNHP RAGNKYRVWP LYNFASAIDD HELGVTHIFR 310 320 330 340 350 360 GQEHAENETR QRYIYEYFGW EYPVTVHHGR LSIEGVILSK SKTRKGIEEG KYLGWDDPRL 370 380 390 400 410 420 GTIRALRRRG ILPEAIKELI IEVGLKKSDA TVSWDNLAAI NRKLVDPIAN RYFFVADPVP 430 440 450 460 470 480 MEVEGAPEFI AKIPLHPDHP ERGTRELRFT PGKPIYVSKD DLDLLKPGSF VRLKDLFNVE 490 500 510 520 530 540 IVEVGEKIKA KFHSFEYEIA RKNKWRMIHW VPEGRPCEVI IPEGDELIVR KGLLEKDANV 550 560 570 KAGEIVQFER FGFVRIDKIE GEKVVAIYAH K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.
[2]	"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638." Gao J., Wang J. Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248284 Genomic DNA. Translation: CAB49406.1. HE613800 Genomic DNA. Translation: CCE69868.1.
PIR	G75165.
RefSeq	NP_126175.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V1E3.
ModBase	Search...
Protein-protein interaction databases
STRING	272844.PAB0323.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB49406; CAB49406; PAB0323.
GeneID	1495381.
KEGG	pab:PAB0323.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000073585.
KO	K01885.
OMA	MRFAPNP.
ProtClustDB	PRK04156.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_A. Glu_tRNA_synth_A.
InterPro	IPR004526. Glu-tRNA-synth_Ib_arc/euk. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR022888. Glu_tRNA_synth_arc. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00463. gltX_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PYRAB

Accession

Primary (citable) accession number: Q9V1E3
Secondary accession number(s): G8ZGI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program