Reviewed, UniProtKB/Swiss-Prot Q9V168 (Y560_PYRAB)

Names and origin

Protein names

Recommended name:
    UPF0027 protein PYRAB05600
Cleaved into the following chain:
    1- Recommended name:
            Pab hyp2 intein
              EC=3.1.-.-

Gene names

Ordered Locus Names:	PYRAB05600
ORF Names:	PAB0383

Organism

Pyrococcus abyssi [Complete proteome] [HAMAP]

Taxonomic identifier

29292 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	916 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the UPF0027 (rtcB) family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing
Ligand	DNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 97	97	UPF0027 protein PYRAB05600, 1st part Potential		PRO_0000232529
	Chain	98 – 533	436	Pab hyp2 intein Potential		PRO_0000232530
	Chain	534 – 916	383	UPF0027 protein PYRAB05600, 2nd part Potential		PRO_0000232531
Regions
	Domain	248 – 409	162	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	Q9V168-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 32DB1F28BE85155A	FASTA	916	102,456
	10 20 30 40 50 60 MTVPLKRIDK IRWEIPKFDK RMRVPGRVYA DDVLLEKMKS DRTLEQAANV AMLPGIYKYS 70 80 90 100 110 120 IVMPDGHQGY GFPIGGVAAF DVNEGVISPG GIGYDINCLA PGSKVLTEHG YWLKVEELPE 130 140 150 160 170 180 KFKLQGVKVY NLDEGHNDTS NVAFVAEREV ETGEMAVRVT TESGRIIEGS EDHPVLTPEG 190 200 210 220 230 240 YVYLGNLKEG NLVIVYPFEG VEYEERKGVI LDEDAFKDED PQVLSFLREK GLVPLRWDDP 250 260 270 280 290 300 RIGTIARILG FAFGDGYLGE MGGRLTLTFY GKEETLRELK KDLERLGISA NLYVRESIET 310 320 330 340 350 360 TSGHSEGKSL SIELRVTSRS FALFLEKLGM PRGKKTEKAY RVPGWILEAP LWVKRNFLAG 370 380 390 400 410 420 LFAADGSIVE FKGNTPLPIN LTQSKSDELA ENLVEFLGDV AKLLAEFGIE TTLYEVKSKK 430 440 450 460 470 480 GVTYRLSIVG EDSIRTFVER INYEYDPEKK VKGLIAAAYL KLKERIVKEA HEAVKDDFPT 490 500 510 520 530 540 FEEFAKERGY EGGFVAEKVV KVERVKPEYT KFYDIGVYHE AHNFIANGIV VHNCGVRLIR 550 560 570 580 590 600 TNLTEKDVRP RIKQLVDTLF KNVPSGVGSQ GRVRLHWTQI DDVLVDGAKW AVDNGYGWEE 610 620 630 640 650 660 DLERLEEGGR MEGADPDAVS QRAKQRGAPQ LGSLGSGNHF LEVQVVDKIF DPEVAKVYGL 670 680 690 700 710 720 FEGQVVVMVH TGSRGLGHQV ASDYLRIMER AIRKYRIPWP DRELVSVPFQ SEEGQRYFSA 730 740 750 760 770 780 MKAAANFAWA NRQMITHWVR ESFQEVFRQD PEDLGMSIVY DVAHNIGKVE EHEVDGKKVK 790 800 810 820 830 840 VIVHRKGATR AFPPGHEAIP KIYRDVGQPV LIPGSMGTAS YVLAGTEGAM KETFGSTCHG 850 860 870 880 890 900 AGRVLSRKAA TRQYRGDRIR QELLNRGIYV RAASMRVVAE EAPGAYKNVD NVVKVVSEAG 910 IAKLVARMRP IGVAKG

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ248284 Genomic DNA. Translation: CAB49482.1.
PIR	C75175.
RefSeq	NP_126251.1.
3D structure databases
SMR	Q9V168. Positions 3-98, 533-916.
ModBase	Search...
Genome annotation databases
GeneID	1495463.
GenomeReviews	Gene locus PYRAB05600 in contig AL096836_GR.
KEGG	pab:PAB0383.
NMPDR	fig|272844.1.peg.587.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9V168.
OMA	VHNCGVR
Enzyme and pathway databases
BioCyc	PABY272844:PAB0383-MON.
Family and domain databases
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR001233. UPF0027. [Graphical view]
PANTHER	PTHR11118. UPF0027. 1 hit.
Pfam	PF01139. UPF0027. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y560_PYRAB

Accession

Primary (citable) accession number: Q9V168

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)