PYRAB06230

Functionⁱ

Catalyzes AdoMet-dependent formation of m5C in tRNA. In the presence of protein archease, specifically methylates the cytosine at position 49 (m5C49) of tRNA. In the absence of archease, catalyzes the formation of m5C at many locations in tRNAs or rRNAs.1 Publication

Catalytic activityⁱ

S-adenosyl-L-methionine + cytosine(49) in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine(49) in tRNA precursor.1 Publication

Enzyme regulationⁱ

Substrate specificity and tendency to aggregate are influenced by archease.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	142	S-adenosyl-L-methioninePROSITE-ProRule annotation	1
Binding siteⁱ	169	S-adenosyl-L-methioninePROSITE-ProRule annotation	1
Binding siteⁱ	186	S-adenosyl-L-methioninePROSITE-ProRule annotation	1
Active siteⁱ	239	NucleophilePROSITE-ProRule annotation	1

GO - Molecular functionⁱ

tRNA (cytosine-5-)-methyltransferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 17470432
tRNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 17470432

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

rRNA processing Source: InterPro
tRNA methylation
Source: UniProtKB
Inferred from direct assayⁱ
- 17470432

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, RNA-binding, Transferase, tRNA-binding
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine

Enzyme and pathway databases

BioCycⁱ	PABY272844:G1GT8-679-MONOMER
BRENDAⁱ	2.1.1.B43 5242

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: tRNA (cytosine(49)-C(5))-methyltransferase (EC:2.1.1.-)
Gene namesⁱ	Ordered Locus Names:PYRAB06230 ORF Names:PAB1947
Organismⁱ	Pyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifierⁱ	272844 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus abyssi
Proteomesⁱ	UP000000810 Componentⁱ: Chromosome UP000009139 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm Curated

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000406990	1 – 311	tRNA (cytosine(49)-C(5))-methyltransferaseAdd BLAST		311

Interactionⁱ

Subunit structureⁱ

Forms a tripartite complex with archease and tRNA. Binds only the oligomeric forms of the archease.1 Publication

Protein-protein interaction databases

STRINGⁱ	272844.PAB1947

STRINGⁱ

272844.PAB1947

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9V106
SMRⁱ	Q9V106
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	118 – 124	S-adenosyl-L-methionine bindingPROSITE-ProRule annotation		7

Sequence similaritiesⁱ

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	arCOG00973 Archaea COG0144 LUCA
HOGENOMⁱ	HOG000224258
KEGG Orthology (KO) More...KOⁱ	K22446
OMAⁱ	EASSMIP
Database of Orthologous Groups More...OrthoDBⁱ	POG093Z05L5

Family and domain databases

InterProⁱ	View protein in InterPro IPR031341 Methyltr_RsmF_N IPR001678 MeTrfase_RsmB/NOP2 IPR011023 Nop2p IPR023267 RCMT IPR029063 SAM-dependent_MTases
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01189 Methyltr_RsmB-F, 1 hit PF17125 Methyltr_RsmF_N, 1 hit
PRINTSⁱ	PR02008 RCMTFAMILY
SUPFAMⁱ	SSF53335 SSF53335, 1 hit
TIGRFAMsⁱ	TIGR00446 nop2p, 1 hit
PROSITEⁱ	View protein in PROSITE PS51686 SAM_MT_RSMB_NOP, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9V106-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDYKEEFKKI NKKLVERYSK LDDSEEFWAY LYKPLRPSIR INTLKGNLKE 
        60         70         80         90        100
IKALLEEKFE LEPIPWTKGE GFYIKSYDVN YGQLIEYSLG LIIPQEASSM 
       110        120        130        140        150
IPPVVLDPKP SEVILDMAAA PGSKTTQMAQ YMENEGCIIA NDAKRDRANI 
       160        170        180        190        200
LIANLTRAGV LIAKVTVKDG AYFARYENTF DRVLLDAPCS SVGMIRKNFK 
       210        220        230        240        250
FARTWSIGKV YYHSRLQKRL ILAAYKSLKP GGVLVYSTCT VDPLENEEVV 
       260        270        280        290        300
DFLLQKTDAK LEKVKLPLKT SEPVIEWEGR KYSDEVRKTI RIHPQDNDTE 
       310 
AFYIAKIRKP R

Length:311

Mass (Da):35,747

Last modified:May 1, 2000 - v1

Checksum:ⁱF34CA8839F39B962

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ248284 Genomic DNA Translation: CAB49545.1 HE613800 Genomic DNA Translation: CCE70017.1
PIRⁱ	B75183
NCBI Reference Sequences More...RefSeqⁱ	WP_010867747.1, NC_000868.1

Genome annotation databases

EnsemblBacteriaⁱ	CAB49545; CAB49545; PAB1947
GeneIDⁱ	1495530
KEGGⁱ	pab:PAB1947
PATRICⁱ	fig\|272844.11.peg.663

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9V106	A0A172WJA7 I6V039 Q8U0N3 A0A075LRL7 F8AH07 D3S2T5 A0A1Z2TM97 UPI000B59B769 UPI000B59A73C A0A0Q2S4T7 +39	Thermococcus piezophilus Pyrococcus furiosus COM1 Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) Palaeococcus pacificus DY20341 Pyrococcus yayanosii (strain CH1 / JCM 16557) Ferroglobus placidus (strain DSM 10642 / AEDII12DO) Thermococcus sp. 5-4 Thermococcus barossii Thermococcus radiotolerans Thermococcus thioreducens And more	311	UniRef50_Q9V106	Cluster: tRNA (cytosine(49)-C(5))-methyltransferase	50

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ248284 Genomic DNA Translation: CAB49545.1 HE613800 Genomic DNA Translation: CCE70017.1
PIRⁱ	B75183
NCBI Reference Sequences More...RefSeqⁱ	WP_010867747.1, NC_000868.1

3D structure databases

ProteinModelPortalⁱ	Q9V106
SMRⁱ	Q9V106
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	272844.PAB1947

STRINGⁱ

272844.PAB1947

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	CAB49545; CAB49545; PAB1947
GeneIDⁱ	1495530
KEGGⁱ	pab:PAB1947
PATRICⁱ	fig\|272844.11.peg.663

Phylogenomic databases

eggNOGⁱ	arCOG00973 Archaea COG0144 LUCA
HOGENOMⁱ	HOG000224258
KEGG Orthology (KO) More...KOⁱ	K22446
OMAⁱ	EASSMIP
Database of Orthologous Groups More...OrthoDBⁱ	POG093Z05L5

Enzyme and pathway databases

BioCycⁱ	PABY272844:G1GT8-679-MONOMER
BRENDAⁱ	2.1.1.B43 5242

Family and domain databases

InterProⁱ	View protein in InterPro IPR031341 Methyltr_RsmF_N IPR001678 MeTrfase_RsmB/NOP2 IPR011023 Nop2p IPR023267 RCMT IPR029063 SAM-dependent_MTases
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01189 Methyltr_RsmB-F, 1 hit PF17125 Methyltr_RsmF_N, 1 hit
PRINTSⁱ	PR02008 RCMTFAMILY
SUPFAMⁱ	SSF53335 SSF53335, 1 hit
TIGRFAMsⁱ	TIGR00446 nop2p, 1 hit
PROSITEⁱ	View protein in PROSITE PS51686 SAM_MT_RSMB_NOP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TRCM_PYRAB
Accessionⁱ	Q9V106Primary (citable) accession number: Q9V106 Secondary accession number(s): G8ZJ90
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 5, 2011
	Last sequence update:	May 1, 2000
	Last modified:	April 25, 2018
	This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9V106 (TRCM_PYRAB)

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tRNA (cytosine(49)-C(5))-methyltransferase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Enzyme regulationⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ