Q9V0Z6 (RTCA_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	PYRAB06330
ORF Names:	PAB1943

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156430
Regions
	Nucleotide binding	283 – 287	5	ATP By similarity
Sites
	Active site	307	1	Tele-AMP-histidine intermediate By similarity
	Binding site	100	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V0Z6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F9B55209F5C07849	FASTA	342	36,928
	10 20 30 40 50 60 MITIDGSYGE GGGQILRTSI ALSAITGEPV RIINIRANRP NPGLRPQHLH GILALKHLAN 70 80 90 100 110 120 ADVKGAHVGS RELVFIPKRL EAKKVEVNIG TAGSITLVLQ ALLPAMAFAK NRVEFKITGG 130 140 150 160 170 180 TDVPWSPPVD YLANVTLFAL EKLGIMAGIK IVRRGHYPKG GGIIEGYVEP WKERRELVAT 190 200 210 220 230 240 KYSSIAKVEG ISHATNLPAH VAERQAKAAK EELSKLEVPV KIKTEVSKSL GPGSGIVVWA 250 260 270 280 290 300 ETDCLRLGGD ALGKRGKPAE VVGKEAAQEL LEQLKPGYCV DKFLGDQLIP FLAFSGGEIW 310 320 330 340 VSEVTNHLKT NIWVVENFLG KVFDLDGEVG KPGKVKVVRR VE

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248284 Genomic DNA. Translation: CAB49555.1.
PIR	D75184.
RefSeq	NP_126324.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V0Z6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000003814; EBPYRP00000003745; EBPYRG00000003814.
GeneID	1495540.
GenomeReviews	Gene locus PYRAB06330 in contig AL096836_GR.
KEGG	pab:PAB1943.
NMPDR	fig|272844.1.peg.664.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022634.
HOGENOM	HBG683539.
OMA	GGTDVAW.
PhylomeDB	Q9V0Z6.
ProtClustDB	PRK04204.
Enzyme and pathway databases
BioCyc	PABY272844:PAB1943-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_PYRAB

Accession

Primary (citable) accession number: Q9V0Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program