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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).By similarity

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPBy similarity1
Active sitei307Tele-AMP-histidine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 287ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPABY272844:G1GT8-689-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
Name:rtcA
Ordered Locus Names:PYRAB06330
ORF Names:PAB1943
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564301 – 342RNA 3'-terminal phosphate cyclaseAdd BLAST342

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB1943

Structurei

3D structure databases

ProteinModelPortaliQ9V0Z6
SMRiQ9V0Z6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG04125 Archaea
COG0430 LUCA
HOGENOMiHOG000015264
KOiK18105
OMAiANRPKPG
OrthoDBiPOG093Z05RD

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF55205 SSF55205, 1 hit
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

Q9V0Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITIDGSYGE GGGQILRTSI ALSAITGEPV RIINIRANRP NPGLRPQHLH
60 70 80 90 100
GILALKHLAN ADVKGAHVGS RELVFIPKRL EAKKVEVNIG TAGSITLVLQ
110 120 130 140 150
ALLPAMAFAK NRVEFKITGG TDVPWSPPVD YLANVTLFAL EKLGIMAGIK
160 170 180 190 200
IVRRGHYPKG GGIIEGYVEP WKERRELVAT KYSSIAKVEG ISHATNLPAH
210 220 230 240 250
VAERQAKAAK EELSKLEVPV KIKTEVSKSL GPGSGIVVWA ETDCLRLGGD
260 270 280 290 300
ALGKRGKPAE VVGKEAAQEL LEQLKPGYCV DKFLGDQLIP FLAFSGGEIW
310 320 330 340
VSEVTNHLKT NIWVVENFLG KVFDLDGEVG KPGKVKVVRR VE
Length:342
Mass (Da):36,928
Last modified:May 1, 2000 - v1
Checksum:iF9B55209F5C07849
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248284 Genomic DNA Translation: CAB49555.1
HE613800 Genomic DNA Translation: CCE70027.1
PIRiD75184
RefSeqiWP_010867757.1, NC_000868.1

Genome annotation databases

EnsemblBacteriaiCAB49555; CAB49555; PAB1943
GeneIDi1495540
KEGGipab:PAB1943
PATRICifig|272844.11.peg.673

Similar proteinsi

Entry informationi

Entry nameiRTCA_PYRAB
AccessioniPrimary (citable) accession number: Q9V0Z6
Secondary accession number(s): G8ZJA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health