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Q9V0Z6 (RTCA_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase

Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4
Gene names
Name:rtcA
Ordered Locus Names:PYRAB06330
ORF Names:PAB1943
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length342 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00200.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 342342RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
PRO_0000156430

Regions

Nucleotide binding283 – 2875ATP By similarity

Sites

Active site3071Tele-AMP-histidine intermediate By similarity
Binding site1001ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9V0Z6 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: F9B55209F5C07849

FASTA34236,928
        10         20         30         40         50         60 
MITIDGSYGE GGGQILRTSI ALSAITGEPV RIINIRANRP NPGLRPQHLH GILALKHLAN 

        70         80         90        100        110        120 
ADVKGAHVGS RELVFIPKRL EAKKVEVNIG TAGSITLVLQ ALLPAMAFAK NRVEFKITGG 

       130        140        150        160        170        180 
TDVPWSPPVD YLANVTLFAL EKLGIMAGIK IVRRGHYPKG GGIIEGYVEP WKERRELVAT 

       190        200        210        220        230        240 
KYSSIAKVEG ISHATNLPAH VAERQAKAAK EELSKLEVPV KIKTEVSKSL GPGSGIVVWA 

       250        260        270        280        290        300 
ETDCLRLGGD ALGKRGKPAE VVGKEAAQEL LEQLKPGYCV DKFLGDQLIP FLAFSGGEIW 

       310        320        330        340 
VSEVTNHLKT NIWVVENFLG KVFDLDGEVG KPGKVKVVRR VE 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248284 Genomic DNA. Translation: CAB49555.1.
HE613800 Genomic DNA. Translation: CCE70027.1.
PIRD75184.
RefSeqNP_126324.1. NC_000868.1.

3D structure databases

ProteinModelPortalQ9V0Z6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272844.PAB1943.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB49555; CAB49555; PAB1943.
GeneID1495540.
KEGGpab:PAB1943.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0430.
HOGENOMHOG000015264.
KOK01974.
OMARRGHYPK.
ProtClustDBPRK04204.

Family and domain databases

Gene3D3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPMF_00200. RTC.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMSSF55205. SSF55205. 1 hit.
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCA_PYRAB
AccessionPrimary (citable) accession number: Q9V0Z6
Secondary accession number(s): G8ZJA0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: February 19, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families