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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit E

Gene

gatE

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit EUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Glu-ADT subunit EUniRule annotation
Gene namesi
Name:gatEUniRule annotation
Ordered Locus Names:PYRAB06990
ORF Names:PAB1902
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001400761 – 633Glutamyl-tRNA(Gln) amidotransferase subunit EAdd BLAST633

Interactioni

Subunit structurei

Heterodimer of GatD and GatE.UniRule annotation

Protein-protein interaction databases

DIPiDIP-48454N.
STRINGi272844.PAB1902.

Structurei

Secondary structure

1633
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni10 – 14Combined sources5
Beta strandi17 – 28Combined sources12
Beta strandi44 – 48Combined sources5
Helixi62 – 69Combined sources8
Beta strandi73 – 78Combined sources6
Turni80 – 82Combined sources3
Helixi85 – 87Combined sources3
Helixi98 – 110Combined sources13
Beta strandi117 – 119Combined sources3
Beta strandi122 – 125Combined sources4
Beta strandi127 – 131Combined sources5
Beta strandi134 – 138Combined sources5
Beta strandi140 – 147Combined sources8
Beta strandi154 – 163Combined sources10
Beta strandi167 – 172Combined sources6
Beta strandi175 – 180Combined sources6
Turni181 – 184Combined sources4
Beta strandi186 – 191Combined sources6
Helixi199 – 215Combined sources17
Beta strandi226 – 232Combined sources7
Beta strandi240 – 243Combined sources4
Helixi248 – 250Combined sources3
Helixi251 – 275Combined sources25
Helixi279 – 281Combined sources3
Turni290 – 295Combined sources6
Helixi299 – 306Combined sources8
Beta strandi310 – 313Combined sources4
Turni320 – 322Combined sources3
Beta strandi323 – 328Combined sources6
Helixi333 – 341Combined sources9
Turni342 – 344Combined sources3
Helixi351 – 353Combined sources3
Helixi361 – 370Combined sources10
Beta strandi378 – 385Combined sources8
Helixi386 – 405Combined sources20
Beta strandi411 – 414Combined sources4
Beta strandi420 – 424Combined sources5
Beta strandi432 – 434Combined sources3
Helixi445 – 452Combined sources8
Helixi459 – 467Combined sources9
Turni468 – 470Combined sources3
Helixi474 – 482Combined sources9
Helixi486 – 494Combined sources9
Helixi501 – 508Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZQ1X-ray3.00C/D1-633[»]
ProteinModelPortaliQ9V0U0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9V0U0.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01719. Archaea.
COG2511. LUCA.
HOGENOMiHOG000222914.
KOiK03330.
OMAiTSGFQRT.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00588. GatE. 1 hit.
InterProiIPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR023168. GatB_Yqey_C.
IPR004414. GatE.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 3 hits.
PfamiPF02938. GAD. 1 hit.
PF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF55261. SSF55261. 1 hit.
SSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00134. gatE_arch. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9V0U0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVMTDKFNY EELGLKVGLE IHRQLDTKKL FSPVPSELSD KVEFTFQRRL
60 70 80 90 100
RPTMSELGEI DPAALEEFKK GRVYVYEGNY ELTDLVYMDE EPPRGPDREA
110 120 130 140 150
LEVALQIAYL LNAKPVDEVY YMRKIVIDGS NVSGFQRTAI IATDGKVETP
160 170 180 190 200
WGAVGIPTIC LEEDAARIIE RKDKEVIYRL DRLGIPLIEI STTPDIHHPE
210 220 230 240 250
QAKVVAKFIG DALRATKKVK RGLGTIRQDL NVSIKGGARI EIKGVQELDM
260 270 280 290 300
IPIIIEREVE RQLNLLKIRD ELRKRGVKPK DIKEEFYDVT DIFENTKSKI
310 320 330 340 350
IARVIKKGGK VLAIKLPKFR GLIGREIQPG RRLGTEFADR AKKYVPGIFH
360 370 380 390 400
IDELPNYGIS QEEVNKVIER LNLSEEDAFV LVAAEEEKAK NALREVIKRA
410 420 430 440 450
REAIEGVPEE TRRALPDGNT EYMRPLPGKA RMYPETDIPP LRIPDDLKKK
460 470 480 490 500
IKENLPELPQ AKVERYVKEY KLDRSLAQTL VDDERDELFE ELVSMGVKPS
510 520 530 540 550
LAASILVVVL KGLRKEVPIE NVTDEHIREA FQLYLEGKIA KEAFEEIFKE
560 570 580 590 600
LARNPSKSAR EVAEEKGLTL LSEEEVTRII EEVIQQNIEV VKAKGMGAMG
610 620 630
LIMGRVMAKV RGKADGKLVS QIVRRKLQEI SGG
Length:633
Mass (Da):71,876
Last modified:May 1, 2000 - v1
Checksum:i3F1BE701DBA6214A
GO

Sequence cautioni

The sequence CCE70090 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248285 Genomic DNA. Translation: CAB49613.1.
HE613800 Genomic DNA. Translation: CCE70090.1. Different initiation.
PIRiD75112.
RefSeqiWP_048146615.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB49613; CAB49613; PAB1902.
GeneIDi1495603.
KEGGipab:PAB1902.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248285 Genomic DNA. Translation: CAB49613.1.
HE613800 Genomic DNA. Translation: CCE70090.1. Different initiation.
PIRiD75112.
RefSeqiWP_048146615.1. NC_000868.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZQ1X-ray3.00C/D1-633[»]
ProteinModelPortaliQ9V0U0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48454N.
STRINGi272844.PAB1902.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB49613; CAB49613; PAB1902.
GeneIDi1495603.
KEGGipab:PAB1902.

Phylogenomic databases

eggNOGiarCOG01719. Archaea.
COG2511. LUCA.
HOGENOMiHOG000222914.
KOiK03330.
OMAiTSGFQRT.

Miscellaneous databases

EvolutionaryTraceiQ9V0U0.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00588. GatE. 1 hit.
InterProiIPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR023168. GatB_Yqey_C.
IPR004414. GatE.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 3 hits.
PfamiPF02938. GAD. 1 hit.
PF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF55261. SSF55261. 1 hit.
SSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00134. gatE_arch. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATE_PYRAB
AccessioniPrimary (citable) accession number: Q9V0U0
Secondary accession number(s): G8ZJG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.