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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit E

Gene

gatE

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit EUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Glu-ADT subunit EUniRule annotation
Gene namesi
Name:gatEUniRule annotation
Ordered Locus Names:PYRAB06990
ORF Names:PAB1902
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 633633Glutamyl-tRNA(Gln) amidotransferase subunit EPRO_0000140076Add
BLAST

Interactioni

Subunit structurei

Heterodimer of GatD and GatE.UniRule annotation

Protein-protein interaction databases

DIPiDIP-48454N.
STRINGi272844.PAB1902.

Structurei

Secondary structure

1
633
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni10 – 145Combined sources
Beta strandi17 – 2812Combined sources
Beta strandi44 – 485Combined sources
Helixi62 – 698Combined sources
Beta strandi73 – 786Combined sources
Turni80 – 823Combined sources
Helixi85 – 873Combined sources
Helixi98 – 11013Combined sources
Beta strandi117 – 1193Combined sources
Beta strandi122 – 1254Combined sources
Beta strandi127 – 1315Combined sources
Beta strandi134 – 1385Combined sources
Beta strandi140 – 1478Combined sources
Beta strandi154 – 16310Combined sources
Beta strandi167 – 1726Combined sources
Beta strandi175 – 1806Combined sources
Turni181 – 1844Combined sources
Beta strandi186 – 1916Combined sources
Helixi199 – 21517Combined sources
Beta strandi226 – 2327Combined sources
Beta strandi240 – 2434Combined sources
Helixi248 – 2503Combined sources
Helixi251 – 27525Combined sources
Helixi279 – 2813Combined sources
Turni290 – 2956Combined sources
Helixi299 – 3068Combined sources
Beta strandi310 – 3134Combined sources
Turni320 – 3223Combined sources
Beta strandi323 – 3286Combined sources
Helixi333 – 3419Combined sources
Turni342 – 3443Combined sources
Helixi351 – 3533Combined sources
Helixi361 – 37010Combined sources
Beta strandi378 – 3858Combined sources
Helixi386 – 40520Combined sources
Beta strandi411 – 4144Combined sources
Beta strandi420 – 4245Combined sources
Beta strandi432 – 4343Combined sources
Helixi445 – 4528Combined sources
Helixi459 – 4679Combined sources
Turni468 – 4703Combined sources
Helixi474 – 4829Combined sources
Helixi486 – 4949Combined sources
Helixi501 – 5088Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZQ1X-ray3.00C/D1-633[»]
ProteinModelPortaliQ9V0U0.
SMRiQ9V0U0. Positions 5-512.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9V0U0.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01719. Archaea.
COG2511. LUCA.
HOGENOMiHOG000222914.
KOiK03330.
OMAiTSGFQRT.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
HAMAPiMF_00588. GatE.
InterProiIPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR023168. GatB_Yqey_C.
IPR004414. GatE.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 3 hits.
PfamiPF02938. GAD. 1 hit.
PF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF55261. SSF55261. 1 hit.
SSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00134. gatE_arch. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9V0U0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVMTDKFNY EELGLKVGLE IHRQLDTKKL FSPVPSELSD KVEFTFQRRL
60 70 80 90 100
RPTMSELGEI DPAALEEFKK GRVYVYEGNY ELTDLVYMDE EPPRGPDREA
110 120 130 140 150
LEVALQIAYL LNAKPVDEVY YMRKIVIDGS NVSGFQRTAI IATDGKVETP
160 170 180 190 200
WGAVGIPTIC LEEDAARIIE RKDKEVIYRL DRLGIPLIEI STTPDIHHPE
210 220 230 240 250
QAKVVAKFIG DALRATKKVK RGLGTIRQDL NVSIKGGARI EIKGVQELDM
260 270 280 290 300
IPIIIEREVE RQLNLLKIRD ELRKRGVKPK DIKEEFYDVT DIFENTKSKI
310 320 330 340 350
IARVIKKGGK VLAIKLPKFR GLIGREIQPG RRLGTEFADR AKKYVPGIFH
360 370 380 390 400
IDELPNYGIS QEEVNKVIER LNLSEEDAFV LVAAEEEKAK NALREVIKRA
410 420 430 440 450
REAIEGVPEE TRRALPDGNT EYMRPLPGKA RMYPETDIPP LRIPDDLKKK
460 470 480 490 500
IKENLPELPQ AKVERYVKEY KLDRSLAQTL VDDERDELFE ELVSMGVKPS
510 520 530 540 550
LAASILVVVL KGLRKEVPIE NVTDEHIREA FQLYLEGKIA KEAFEEIFKE
560 570 580 590 600
LARNPSKSAR EVAEEKGLTL LSEEEVTRII EEVIQQNIEV VKAKGMGAMG
610 620 630
LIMGRVMAKV RGKADGKLVS QIVRRKLQEI SGG
Length:633
Mass (Da):71,876
Last modified:May 1, 2000 - v1
Checksum:i3F1BE701DBA6214A
GO

Sequence cautioni

The sequence CCE70090.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248285 Genomic DNA. Translation: CAB49613.1.
HE613800 Genomic DNA. Translation: CCE70090.1. Different initiation.
PIRiD75112.

Genome annotation databases

EnsemblBacteriaiCAB49613; CAB49613; PAB1902.
KEGGipab:PAB1902.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248285 Genomic DNA. Translation: CAB49613.1.
HE613800 Genomic DNA. Translation: CCE70090.1. Different initiation.
PIRiD75112.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZQ1X-ray3.00C/D1-633[»]
ProteinModelPortaliQ9V0U0.
SMRiQ9V0U0. Positions 5-512.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48454N.
STRINGi272844.PAB1902.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB49613; CAB49613; PAB1902.
KEGGipab:PAB1902.

Phylogenomic databases

eggNOGiarCOG01719. Archaea.
COG2511. LUCA.
HOGENOMiHOG000222914.
KOiK03330.
OMAiTSGFQRT.

Miscellaneous databases

EvolutionaryTraceiQ9V0U0.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
HAMAPiMF_00588. GatE.
InterProiIPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR023168. GatB_Yqey_C.
IPR004414. GatE.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 3 hits.
PfamiPF02938. GAD. 1 hit.
PF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF55261. SSF55261. 1 hit.
SSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00134. gatE_arch. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiGATE_PYRAB
AccessioniPrimary (citable) accession number: Q9V0U0
Secondary accession number(s): G8ZJG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.