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Q9V0T9 (GATD_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit D
Short name=Glu-ADT subunit D
EC=6.3.5.-
Gene names
Name:gatD
Ordered Locus Names:PYRAB07000
ORF Names:PAB1901
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The gatDE system is specific for glutamate and does not act on aspartate By similarity. HAMAP MF_00586

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00586

Subunit structure

Heterodimer of gatD and gatE By similarity. HAMAP MF_00586

Sequence similarities

Belongs to the asparaginase 1 family. GatD subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438Glutamyl-tRNA(Gln) amidotransferase subunit D HAMAP MF_00586
PRO_0000140058

Sites

Active site1021 By similarity
Active site1781 By similarity
Active site1791 By similarity
Active site2561 By similarity

Secondary structure

1 .......................................................................... 438
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9V0T9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 39C616E89496D61C

FASTA43848,860
        10         20         30         40         50         60 
MRVDEFLKER NINVGDFVRI TKEEDGEEVT YEGYIMPPYE LSAGDTLVLK LENGYNIGIA 

        70         80         90        100        110        120 
LEKIRRIEVL ERAKVKPEVH FEALIEGKPG LPEVTIIGTG GTIASRIDYE TGAVYPAFTA 

       130        140        150        160        170        180 
EELAKAVPEI FEVANVKPKL LFNIFSEDMK PKHWVKIAHE VAKALNSGDY GVVVAHGTDT 

       190        200        210        220        230        240 
MGYTAAALSF MLRNLGKPVV LVGAQRSSDR PSSDAAMNLI CSVRMATSEV AEVMVVMHGE 

       250        260        270        280        290        300 
TGDTYCLAHR GTKVRKMHTS RRDAFRSIND VPIAKIWPNG EIEFLRKDYR KRSDEEVEVD 

       310        320        330        340        350        360 
DKIEEKVALV KVYPGISSEI IDFLVDKGYK GIVIEGTGLG HTPNDIIPSI ERAVEEGVAV 

       370        380        390        400        410        420 
CMTSQCIYGR VNLNVYSTGR KLLKAGVIPC EDMLPETAYV KLMWVLGHTQ NLEEVRKMML 

       430 
TNYAGEITPY TRFDTYLR

« Hide

References

[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ248285 Genomic DNA. Translation: CAB49614.1.
PIRE75112.
RefSeqNP_126383.1. NC_000868.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZQ1X-ray3.00A/B1-438[»]
ProteinModelPortalQ9V0T9.
SMRQ9V0T9. Positions 2-438.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-48453N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000002189; EBPYRP00000002120; EBPYRG00000002189.
GeneID1495604.
GenomeReviewsGene locus PYRAB07000 in contig AL096836_GR.
KEGGpab:PAB1901.
NMPDRfig|272844.1.peg.728.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000022811.
HOGENOMHBG497495.
OMAPEFYHDE.
PhylomeDBQ9V0T9.
ProtClustDBPRK04183.

Enzyme and pathway databases

BioCycPABY272844:PAB1901-MONOMER.

Family and domain databases

HAMAPMF_00586. GatD.
[Tree]
InterProIPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR020827. Asparaginase/glutaminase_CS.
IPR011878. GatD_arch.
[Graphical view]
KOK09482.
PANTHERPTHR11707. Asp/Glutamnse. 1 hit.
PfamPF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSPR00139. ASNGLNASE.
SMARTSM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMSSF53774. Asp/Glutamnse. 1 hit.
TIGRFAMsTIGR00519. AsnASE_I. 1 hit.
TIGR02153. GatD_arch. 1 hit.
PROSITEPS00144. ASN_GLN_ASE_1. 1 hit.
PS00917. ASN_GLN_ASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATD_PYRAB
AccessionPrimary (citable) accession number: Q9V0T9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: May 1, 2000
Last modified: December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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