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Protein

Putative 2-dehydropantoate 2-reductase

Gene

apbA

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.By similarity

Catalytic activityi

(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.

Pathway: (R)-pantothenate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. Putative 2-dehydropantoate 2-reductase (apbA)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991NADP; via amide nitrogenBy similarity
Binding sitei99 – 991SubstrateBy similarity
Active sitei179 – 1791Proton donorBy similarity
Binding sitei183 – 1831SubstrateBy similarity
Binding sitei187 – 1871SubstrateBy similarity
Binding sitei249 – 2491SubstrateBy similarity
Binding sitei261 – 2611NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 126NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pantothenate biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00028; UER00004.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 2-dehydropantoate 2-reductase (EC:1.1.1.169)
Alternative name(s):
Ketopantoate reductase
Short name:
KPA reductase
Short name:
KPR
Gene namesi
Name:apbA
Ordered Locus Names:PYRAB07590
ORF Names:PAB0512
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000810 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Putative 2-dehydropantoate 2-reductasePRO_0000157325Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB0512.

Structurei

3D structure databases

ProteinModelPortaliQ9V0N0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ketopantoate reductase family.Curated

Phylogenomic databases

eggNOGiCOG1893.
HOGENOMiHOG000050222.
KOiK00077.
OMAiWKWAKAI.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR003710. ApbA.
IPR013752. KPA_reductase.
IPR013332. KPR_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02558. ApbA. 1 hit.
PF08546. ApbA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00745. apbA_panE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9V0N0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIYILGAGA IGSLFGGLLA NAGEDVLLIG RDPHVSAINE KGLKIVGIKD
60 70 80 90 100
LNVKVEATTR VPEEKPDLIV LATKSYSTIE ALKSARHIVK GSWVLSIQNG
110 120 130 140 150
IGNEDKIIEF GGKAIGGITT NGAMVEAPGV IKWTGKGVTI IGLYPQGKEK
160 170 180 190 200
FIEKVADVFN SADIETHVSE NIISWIWAKA IVNSAINPIG TLLEVKNKVI
210 220 230 240 250
RENDFLLSMA MEVVKEGCRV ALQNGIEFDV PPMDLFFQTL EQTRENYNSM
260 270 280 290 300
LQDIWRGKKT EVDYINGKIV EYAKAVNLEA PMNLLLWGLI KGKEALEGKK
Length:300
Mass (Da):32,773
Last modified:May 1, 2000 - v1
Checksum:iF559625B73F691DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248285 Genomic DNA. Translation: CAB49673.1.
HE613800 Genomic DNA. Translation: CCE70155.1.
PIRiH75119.
RefSeqiNP_126442.1. NC_000868.1.
WP_010867881.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB49673; CAB49673; PAB0512.
GeneIDi1496099.
KEGGipab:PAB0512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248285 Genomic DNA. Translation: CAB49673.1.
HE613800 Genomic DNA. Translation: CCE70155.1.
PIRiH75119.
RefSeqiNP_126442.1. NC_000868.1.
WP_010867881.1. NC_000868.1.

3D structure databases

ProteinModelPortaliQ9V0N0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272844.PAB0512.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB49673; CAB49673; PAB0512.
GeneIDi1496099.
KEGGipab:PAB0512.

Phylogenomic databases

eggNOGiCOG1893.
HOGENOMiHOG000050222.
KOiK00077.
OMAiWKWAKAI.

Enzyme and pathway databases

UniPathwayiUPA00028; UER00004.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR003710. ApbA.
IPR013752. KPA_reductase.
IPR013332. KPR_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02558. ApbA. 1 hit.
PF08546. ApbA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00745. apbA_panE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiPANE_PYRAB
AccessioniPrimary (citable) accession number: Q9V0N0
Secondary accession number(s): G8ZGW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: May 27, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.