Q9V0I7 (GUAAB_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Synonyms:	guaA-C
Ordered Locus Names:	PYRAB08020
ORF Names:	PAB2417

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_0000140247
Regions
	Domain	1 – 185	185	GMPS ATP-PPase
	Nucleotide binding	28 – 34	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V0I7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D5A3A59B9B3BC3B6	FASTA	308	34,632
	10 20 30 40 50 60 MNWEKFVEEK VKEIRETVGD SKAIIALSGG VDSSTAAVLA YKAIGDKLHA VFVNTGFLRK 70 80 90 100 110 120 GEPEFVVKTF RDEFGMNLHY VDAQDRFFSA LKGVTDPEEK RKIIGRVFIE VFEEVAREIG 130 140 150 160 170 180 AEYLIQGTIA PDWIESQGKI KSHHNVGGLP ERLNLKLIEP LRDLYKDEVR ELAKFLGLPE 190 200 210 220 230 240 KIYNRMPFPG PGLAVRVIGE VTPEKIRIVR EANAIVEEEV ERAGLRPWQA FAVLLGVKTV 250 260 270 280 290 300 GVQGDIRAYK ETIAVRIVES LDGMTANAMN VPWEVLQRIA FRITSEIPEV GRVLYDITNK PPATIEFE

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248285 Genomic DNA. Translation: CAB49716.1.
PIR	C75125.
RefSeq	NP_126485.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V0I7.
SMR	Q9V0I7. Positions 1-308.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000003658; EBPYRP00000003589; EBPYRG00000003658.
GeneID	1496146.
GenomeReviews	Gene locus PYRAB08020 in contig AL096836_GR.
KEGG	pab:PAB2417.
NMPDR	fig|272844.1.peg.839.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022268.
HOGENOM	HBG391775.
OMA	QGTIAPD.
PhylomeDB	Q9V0I7.
ProtClustDB	PRK00919.
Enzyme and pathway databases
BioCyc	PABY272844:PAB2417-MONOMER.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_PYRAB

Accession

Primary (citable) accession number: Q9V0I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program