Q9V0I6 (GUAAA_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	guaAA
Synonyms:	guaA-N
Ordered Locus Names:	PYRAB08030
ORF Names:	PAB0549

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140228
Regions
	Domain	1 – 188	188	Glutamine amidotransferase type-1
Sites
	Active site	78	1	Nucleophile By similarity
	Active site	165	1	By similarity
	Active site	167	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9V0I6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 4446057FC79660B4	FASTA	188	21,317
	10 20 30 40 50 60 MIVIMDNGGQ YVHRIWRTLR YIGVESKIIP NTTPLEDIKA MNPSGIIFSG GPSLENTGNC 70 80 90 100 110 120 EKILENYDEF NVPILGICLG HQLIAKFFGG KVGRGEKAEY SLVEIEILEE DEIFKGLPRK 130 140 150 160 170 180 LRVWESHMDE VKELPPNFKV LARSETCPIE AMKHEELPIY GVQFHPEVAH TEHGEDILRN FAKLCGEL

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248285 Genomic DNA. Translation: CAB49717.1.
PIR	D75125.
RefSeq	NP_126486.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9V0I6.
SMR	Q9V0I6. Positions 1-188.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002356; EBPYRP00000002287; EBPYRG00000002356.
GeneID	1496147.
GenomeReviews	Gene locus PYRAB08030 in contig AL096836_GR.
KEGG	pab:PAB0549.
NMPDR	fig|272844.1.peg.840.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022457.
HOGENOM	HBG292341.
OMA	GQYVHRI.
PhylomeDB	Q9V0I6.
ProtClustDB	PRK00758.
Enzyme and pathway databases
BioCyc	PABY272844:PAB0549-MONOMER.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR017926. GATASE_1. IPR004739. GMP_synth_N. IPR023686. GMP_synthase_A. [Graphical view]
KO	K01951.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_PYRAB

Accession

Primary (citable) accession number: Q9V0I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program