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Q9V0B9 (SYL_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PYRAB08710
ORF Names:PAB1782
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length967 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 967967Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152137

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif650 – 6545"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6531ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9V0B9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: AA138311F00276A7

FASTA967113,824
        10         20         30         40         50         60 
MPELNFRAIE EKWQKRWLEA KVFEPNIKDK PKEKKFYITV AFPYLSGHLH VGHARTYTIP 

        70         80         90        100        110        120 
DVIARFKRMQ GYNVLFPMGW HITGSPIVGI AERIKNRDPH TIWIYRDVYK VPEEILWTFE 

       130        140        150        160        170        180 
DPVNIVKYFM KAAKETFIRA GFSVDWSREF YTTSLFPPFS KFIEWQFLKL KEKGYIVKGA 

       190        200        210        220        230        240 
HRVRWDPVVG TPLGDHDLME GEDVPILEYV IIKFELKEGD ETIYLPAATL RPETVYGVTN 

       250        260        270        280        290        300 
MWINPNATYV KAKVKRGGKE ETWIISKEAA YKLSFQDREI EVIEEFKGEK LIGKYVRNPV 

       310        320        330        340        350        360 
TGDEVIILPA EFVDPDNATG VVMSVPAHAP FDHVALEDLK RESEILVKYD IDPRIVEEIT 

       370        380        390        400        410        420 
YISLIKLEGY GEFPAVEEVQ KLGIKSQKDR EKLEQATKTI YKAEYHKGIF KVPPYDGKPV 

       430        440        450        460        470        480 
QEVKELIAKE MMEKGIAEIM YEFAEKNVIS RFGNRAVIKI IHDQWFIDYG NSEWKEKARK 

       490        500        510        520        530        540 
ALARMKIYPE TRRAQFEAII DWLDKKACAR KVGLGTPLPW DPEWVIESLS DSTIYMAYYT 

       550        560        570        580        590        600 
ISRHINRLRE EGRLDPEKLT PEFFDYIFLE EFSEEREKEL EKKTGIPAEI IHEMKEEFEY 

       610        620        630        640        650        660 
WYPLDWRCSG KDLIPNHLTF FIFNHVAIFR EEHWPKGIAV NGFGTLEGQK MSKSKGNVLN 

       670        680        690        700        710        720 
FIDAIEENGA DVVRLYIMSL AEHDSDFDWR RKEVGKLRRQ LERFYELISQ FAEYEAKENV 

       730        740        750        760        770        780 
ELKTIDKWLL HRLNKAIEGT TKALEEFRTR TAVQWAFYSI MNDLRWYMRR TEGRDDEAKR 

       790        800        810        820        830        840 
FVLRKLADIW VRLMAPFTPH ICEELWEKLG GEGFVSLAKW PEPVDEWWNE EVEVEEDFIK 

       850        860        870        880        890        900 
SLIEDIKEII EVAKIESPKR AYIYTAPEWK WKVYEVVAEK REFKSAMAEL MKDEEIRKHG 

       910        920        930        940        950        960 
KEVAKLVQAI IKERAFDVKR IDEEKVLRES KDFLEKELGL EVIINPEEDK GGKKRQAIPL 


KPAVFIE 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248285 Genomic DNA. Translation: CAB49785.1.
HE613800 Genomic DNA. Translation: CCE70276.1.
PIRH75133.
RefSeqNP_126554.1. NC_000868.1.

3D structure databases

ProteinModelPortalQ9V0B9.
SMRQ9V0B9. Positions 4-967.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272844.PAB1782.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB49785; CAB49785; PAB1782.
GeneID1496220.
KEGGpab:PAB1782.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAIMNDLRW.
ProtClustDBPRK12300.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PYRAB
AccessionPrimary (citable) accession number: Q9V0B9
Secondary accession number(s): G8ZI35
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries