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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei628ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPABY272844:G1GT8-1000-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PYRAB09190
ORF Names:PAB0616
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985881 – 1067Isoleucine--tRNA ligaseAdd BLAST1067

Proteomic databases

PRIDEiQ9V072

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272844.PAB0616

Structurei

3D structure databases

ProteinModelPortaliQ9V072
SMRiQ9V072
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 59"HIGH" regionAdd BLAST11
Motifi625 – 629"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9V072-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKEPEFREY DPKKLEEKVE KFWSENEIYR KVKELRKDGP KYYFLDGPPY
60 70 80 90 100
VSGAIHLGTA WNKIIKDMII RFRTMQGYNV WRQPGYDMHG LPIEVKVEQA
110 120 130 140 150
LGLKTKKEIE EKIGVENFIK KCKEFALNNL KIMTEQFKML GIWMDWDNPY
160 170 180 190 200
MTIKNEYIES AWFTLKRAWE KGLLEKDKRV LHWCPRCETA LAEHEVRGEY
210 220 230 240 250
KLRKDPSIYV KFPVEGKENE YLLIWTTTPW TLPANLAVSA HPDYDYVKVK
260 270 280 290 300
VEFNGREEYW ILAKALVDKV LGEIGVKGEV VEEFKGRELE GLRYVHILMD
310 320 330 340 350
EYPRQKEFKE KYEWAHRVIL ADFVTLEEGT GLVHTAPGHG EEDFEVGQKY
360 370 380 390 400
GLPVYSPLDD QGKYTEGKWK GIYVKEADPK IIEHLREKGY LVKAGEIEHK
410 420 430 440 450
YPHCWRCKTP LIFRATDQWF LKISKVKDKI IKENDEKVTW YPDWVKIRFD
460 470 480 490 500
NGVRDSGDWV ISRQRYWGIP LPIWQSEDGE IYVVGSWREL VELAVAIEVN
510 520 530 540 550
GERIELPESY EEKLKVIEEK LGPEDLHRPY VDAFIIKVNG KDMRRVKDVV
560 570 580 590 600
DVWFDSGIAS WASLGYPRNK ELFEKLWPAD FIVEGEDQVT KWFYSQQAAS
610 620 630 640 650
IVAFDTVPYR RVAMHGYVLD EKGDKMSKSL GNIIRPEEVV ERAGRDTFRF
660 670 680 690 700
YMLWATNPWE NLKFSWKGVE QVRRMLNILW NVYVLASTYM SLDNFDPRKL
710 720 730 740 750
NPDELPFREE DKWILSRVNS LISEVENGIE SFYLTKATRA LYNFVVEDLS
760 770 780 790 800
RWYVRLIRKR LWVEGEDPDK LAAYYTLWKV FDVLLRLLAP FTPYIAEEIY
810 820 830 840 850
QNLIRPFTNV ESVHMLDWPK VDEKAIDEEL EREMEFIRRI VEAGSAARQR
860 870 880 890 900
AKIKLRYPVR RIIIETEDET VKKAVERLNR ILRDQLNAKE VKVGRVEREL
910 920 930 940 950
TIKPNFAKLG PEFKGDAKII AKWINENGRE LYEKGELTVE IDGKTFHLTR
960 970 980 990 1000
EHIIVEEKLP DFFVSEEFEG GRVFVDKTLT RELIAEGLAR EFVRRIQEMR
1010 1020 1030 1040 1050
KRLDLDVNDR IIVTIETTDE NVELLKENLD YIMRETRADK IVFGKAKGYV
1060
VEWPEVQAKI GIEKVEE
Length:1,067
Mass (Da):126,109
Last modified:May 1, 2000 - v1
Checksum:iB7A8C50C93924D82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248285 Genomic DNA Translation: CAB49833.1
HE613800 Genomic DNA Translation: CCE70327.1
PIRiH75139
RefSeqiWP_010868042.1, NC_000868.1

Genome annotation databases

EnsemblBacteriaiCAB49833; CAB49833; PAB0616
GeneIDi1496271
KEGGipab:PAB0616
PATRICifig|272844.11.peg.973

Similar proteinsi

Entry informationi

Entry nameiSYI_PYRAB
AccessioniPrimary (citable) accession number: Q9V072
Secondary accession number(s): G8ZI86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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