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Q9V072

- SYI_PYRAB

UniProt

Q9V072 - SYI_PYRAB

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei628 – 6281ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PYRAB09190
ORF Names:PAB0616
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000810: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10671067Isoleucine--tRNA ligasePRO_0000098588Add
BLAST

Proteomic databases

PRIDEiQ9V072.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272844.PAB0616.

Structurei

3D structure databases

ProteinModelPortaliQ9V072.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 5911"HIGH" regionAdd
BLAST
Motifi625 – 6295"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9V072-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKEPEFREY DPKKLEEKVE KFWSENEIYR KVKELRKDGP KYYFLDGPPY
60 70 80 90 100
VSGAIHLGTA WNKIIKDMII RFRTMQGYNV WRQPGYDMHG LPIEVKVEQA
110 120 130 140 150
LGLKTKKEIE EKIGVENFIK KCKEFALNNL KIMTEQFKML GIWMDWDNPY
160 170 180 190 200
MTIKNEYIES AWFTLKRAWE KGLLEKDKRV LHWCPRCETA LAEHEVRGEY
210 220 230 240 250
KLRKDPSIYV KFPVEGKENE YLLIWTTTPW TLPANLAVSA HPDYDYVKVK
260 270 280 290 300
VEFNGREEYW ILAKALVDKV LGEIGVKGEV VEEFKGRELE GLRYVHILMD
310 320 330 340 350
EYPRQKEFKE KYEWAHRVIL ADFVTLEEGT GLVHTAPGHG EEDFEVGQKY
360 370 380 390 400
GLPVYSPLDD QGKYTEGKWK GIYVKEADPK IIEHLREKGY LVKAGEIEHK
410 420 430 440 450
YPHCWRCKTP LIFRATDQWF LKISKVKDKI IKENDEKVTW YPDWVKIRFD
460 470 480 490 500
NGVRDSGDWV ISRQRYWGIP LPIWQSEDGE IYVVGSWREL VELAVAIEVN
510 520 530 540 550
GERIELPESY EEKLKVIEEK LGPEDLHRPY VDAFIIKVNG KDMRRVKDVV
560 570 580 590 600
DVWFDSGIAS WASLGYPRNK ELFEKLWPAD FIVEGEDQVT KWFYSQQAAS
610 620 630 640 650
IVAFDTVPYR RVAMHGYVLD EKGDKMSKSL GNIIRPEEVV ERAGRDTFRF
660 670 680 690 700
YMLWATNPWE NLKFSWKGVE QVRRMLNILW NVYVLASTYM SLDNFDPRKL
710 720 730 740 750
NPDELPFREE DKWILSRVNS LISEVENGIE SFYLTKATRA LYNFVVEDLS
760 770 780 790 800
RWYVRLIRKR LWVEGEDPDK LAAYYTLWKV FDVLLRLLAP FTPYIAEEIY
810 820 830 840 850
QNLIRPFTNV ESVHMLDWPK VDEKAIDEEL EREMEFIRRI VEAGSAARQR
860 870 880 890 900
AKIKLRYPVR RIIIETEDET VKKAVERLNR ILRDQLNAKE VKVGRVEREL
910 920 930 940 950
TIKPNFAKLG PEFKGDAKII AKWINENGRE LYEKGELTVE IDGKTFHLTR
960 970 980 990 1000
EHIIVEEKLP DFFVSEEFEG GRVFVDKTLT RELIAEGLAR EFVRRIQEMR
1010 1020 1030 1040 1050
KRLDLDVNDR IIVTIETTDE NVELLKENLD YIMRETRADK IVFGKAKGYV
1060
VEWPEVQAKI GIEKVEE
Length:1,067
Mass (Da):126,109
Last modified:May 1, 2000 - v1
Checksum:iB7A8C50C93924D82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248285 Genomic DNA. Translation: CAB49833.1.
HE613800 Genomic DNA. Translation: CCE70327.1.
PIRiH75139.
RefSeqiNP_126602.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB49833; CAB49833; PAB0616.
GeneIDi1496271.
KEGGipab:PAB0616.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248285 Genomic DNA. Translation: CAB49833.1 .
HE613800 Genomic DNA. Translation: CCE70327.1 .
PIRi H75139.
RefSeqi NP_126602.1. NC_000868.1.

3D structure databases

ProteinModelPortali Q9V072.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272844.PAB0616.

Proteomic databases

PRIDEi Q9V072.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB49833 ; CAB49833 ; PAB0616 .
GeneIDi 1496271.
KEGGi pab:PAB0616.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiSYI_PYRAB
AccessioniPrimary (citable) accession number: Q9V072
Secondary accession number(s): G8ZI86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3