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Q9V011 (SYM_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Synonyms:metS
Ordered Locus Names:PYRAB09870
ORF Names:PAB2364
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length722 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 722722Methionine--tRNA ligase HAMAP MF_00098
PRO_0000139194

Regions

Domain622 – 722101tRNA-binding
Motif11 – 2111"HIGH" region HAMAP MF_00098
Motif344 – 3485"KMSKS" region HAMAP MF_00098

Sites

Metal binding1431Zinc By similarity
Metal binding1461Zinc By similarity
Metal binding1561Zinc By similarity
Metal binding1591Zinc By similarity
Binding site3471ATP By similarity

Secondary structure

1 ................................................................................................................... 722
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9V011 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: A69078A91D5BE028

FASTA72284,295
        10         20         30         40         50         60 
MVRYMVTSAL PYANGPIHAG HLAGAYLPAD IFVRYLRLKG EDVVFICGTD EHGTPISFRA 

        70         80         90        100        110        120 
LKEGRSPREI VDEFHEQIKI TFQRAKISFD FFGRTELPIH YKLSQEFFLK AYENGHLVKK 

       130        140        150        160        170        180 
VTKQAYCEHD KMFLPDRFVI GTCPYCGAED QKGDQCEVCG RPLTPEILIN PRCAICGRPI 

       190        200        210        220        230        240 
SFRDSAHYYI KMQDFAERLK RWIEKQPWKP NVKNMVLSWI EEGLEERAIT RDLNWGIPVP 

       250        260        270        280        290        300 
LDEEDMKGKV LYVWFEAPIG YISITIEHFK RIGKPNEWKK YWLNIDGQTR VIHFIGKDNI 

       310        320        330        340        350        360 
PFHAIFWPAF LMAYGKYKDE EVEAEWNLPY DIPANEYLTL EGKKFSTSRN WAIWVHEFLD 

       370        380        390        400        410        420 
VFPADYLRYY LTTIMPETRD SDFSFSDFKV RINEELVNNL GNFVHRALTF VNRYFDGVVP 

       430        440        450        460        470        480 
ERGELDELDR EALEEIEKAF KEVGELIMNY RFKDALKRVM SLASFGNRYF DHKQPWKTAK 

       490        500        510        520        530        540 
EDKVRTGTTV NISLQIVKAL GILLEPFLPD ASEKIWHLLN LDEVKRWEFR ELPAGHKVRK 

       550        560        570        580        590        600 
PEILFKKVTD DQIIYFILNY MAKGNPEGAR ILLDKYYKRE DVIRVAKEKF GDEAEVVLRR 

       610        620        630        640        650        660 
VYKDIKLKEK KEGKEMYVKF DDFAKLDLRV GKIIEVKDHP NADKLYVVKV DLGDEVRTLV 

       670        680        690        700        710        720 
AGLKKYYKPE ELLNRYVVVV ANLEPKKLRG IGSQGMLLAA DDGERVALLM PDKEVKLGAK 


VR

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References

[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ248286 Genomic DNA. Translation: CAB49895.1.
PIRB75074.
RefSeqNP_126664.1. NC_000868.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MKHX-ray2.01A616-722[»]
1RQGX-ray2.90A1-722[»]
ProteinModelPortalQ9V011.
SMRQ9V011. Positions 1-606, 616-722.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000002144; EBPYRP00000002075; EBPYRG00000002144.
GeneID1496337.
GenomeReviewsGene locus PYRAB09870 in contig AL096836_GR.
KEGGpab:PAB2364.
NMPDRfig|272844.1.peg.1030.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00070000032183.
HOGENOMHBG721667.
OMAHEIAPFK.
PhylomeDBQ9V011.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycPABY272844:PAB2364-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_PYRAB
AccessionPrimary (citable) accession number: Q9V011
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: January 25, 2012
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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