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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi143 – 1431ZincUniRule annotation
Metal bindingi146 – 1461ZincUniRule annotation
Metal bindingi156 – 1561ZincUniRule annotation
Metal bindingi159 – 1591ZincUniRule annotation
Binding sitei347 – 3471ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BRENDAi6.1.1.10. 5242.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotation
Synonyms:metS
Ordered Locus Names:PYRAB09870
ORF Names:PAB2364
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 722722Methionine--tRNA ligasePRO_0000139194Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272844.PAB2364.

Structurei

Secondary structure

1
722
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi19 – 257Combined sources
Helixi27 – 3812Combined sources
Beta strandi42 – 509Combined sources
Helixi54 – 6310Combined sources
Helixi67 – 8519Combined sources
Beta strandi90 – 945Combined sources
Helixi98 – 11316Combined sources
Beta strandi117 – 12711Combined sources
Turni128 – 1314Combined sources
Helixi136 – 1383Combined sources
Beta strandi144 – 1463Combined sources
Turni153 – 1553Combined sources
Beta strandi157 – 1593Combined sources
Beta strandi167 – 1704Combined sources
Turni174 – 1763Combined sources
Beta strandi181 – 19010Combined sources
Helixi192 – 1943Combined sources
Helixi196 – 2049Combined sources
Helixi210 – 22011Combined sources
Beta strandi234 – 2363Combined sources
Beta strandi244 – 2463Combined sources
Helixi253 – 2564Combined sources
Helixi257 – 2593Combined sources
Helixi260 – 27112Combined sources
Turni275 – 2784Combined sources
Helixi279 – 2824Combined sources
Beta strandi289 – 2968Combined sources
Helixi297 – 2993Combined sources
Helixi300 – 3045Combined sources
Helixi306 – 3127Combined sources
Beta strandi319 – 3213Combined sources
Beta strandi338 – 3403Combined sources
Turni347 – 3504Combined sources
Helixi355 – 3584Combined sources
Turni359 – 3613Combined sources
Helixi364 – 37310Combined sources
Beta strandi377 – 3793Combined sources
Beta strandi381 – 3833Combined sources
Helixi385 – 39410Combined sources
Helixi395 – 3995Combined sources
Helixi400 – 41314Combined sources
Helixi427 – 44822Combined sources
Helixi452 – 47120Combined sources
Helixi475 – 4784Combined sources
Turni479 – 4813Combined sources
Helixi483 – 50422Combined sources
Turni505 – 5073Combined sources
Helixi509 – 51810Combined sources
Helixi550 – 55910Combined sources
Turni560 – 5645Combined sources
Helixi566 – 57611Combined sources
Helixi579 – 59012Combined sources
Helixi591 – 5944Combined sources
Helixi595 – 6017Combined sources
Helixi620 – 6245Combined sources
Beta strandi628 – 63811Combined sources
Beta strandi646 – 6516Combined sources
Beta strandi656 – 6616Combined sources
Turni664 – 6663Combined sources
Helixi669 – 6724Combined sources
Beta strandi676 – 6805Combined sources
Beta strandi699 – 7013Combined sources
Beta strandi706 – 7083Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MKHX-ray2.01A616-722[»]
1RQGX-ray2.90A1-722[»]
ProteinModelPortaliQ9V011.
SMRiQ9V011. Positions 1-606, 616-722.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9V011.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini622 – 722101tRNA-bindingUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi344 – 3485"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00810. Archaea.
COG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200402.
KOiK01874.
OMAiFWPAMLD.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR004495. Met-tRNA-synth_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9V011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRYMVTSAL PYANGPIHAG HLAGAYLPAD IFVRYLRLKG EDVVFICGTD
60 70 80 90 100
EHGTPISFRA LKEGRSPREI VDEFHEQIKI TFQRAKISFD FFGRTELPIH
110 120 130 140 150
YKLSQEFFLK AYENGHLVKK VTKQAYCEHD KMFLPDRFVI GTCPYCGAED
160 170 180 190 200
QKGDQCEVCG RPLTPEILIN PRCAICGRPI SFRDSAHYYI KMQDFAERLK
210 220 230 240 250
RWIEKQPWKP NVKNMVLSWI EEGLEERAIT RDLNWGIPVP LDEEDMKGKV
260 270 280 290 300
LYVWFEAPIG YISITIEHFK RIGKPNEWKK YWLNIDGQTR VIHFIGKDNI
310 320 330 340 350
PFHAIFWPAF LMAYGKYKDE EVEAEWNLPY DIPANEYLTL EGKKFSTSRN
360 370 380 390 400
WAIWVHEFLD VFPADYLRYY LTTIMPETRD SDFSFSDFKV RINEELVNNL
410 420 430 440 450
GNFVHRALTF VNRYFDGVVP ERGELDELDR EALEEIEKAF KEVGELIMNY
460 470 480 490 500
RFKDALKRVM SLASFGNRYF DHKQPWKTAK EDKVRTGTTV NISLQIVKAL
510 520 530 540 550
GILLEPFLPD ASEKIWHLLN LDEVKRWEFR ELPAGHKVRK PEILFKKVTD
560 570 580 590 600
DQIIYFILNY MAKGNPEGAR ILLDKYYKRE DVIRVAKEKF GDEAEVVLRR
610 620 630 640 650
VYKDIKLKEK KEGKEMYVKF DDFAKLDLRV GKIIEVKDHP NADKLYVVKV
660 670 680 690 700
DLGDEVRTLV AGLKKYYKPE ELLNRYVVVV ANLEPKKLRG IGSQGMLLAA
710 720
DDGERVALLM PDKEVKLGAK VR
Length:722
Mass (Da):84,295
Last modified:May 1, 2000 - v1
Checksum:iA69078A91D5BE028
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248286 Genomic DNA. Translation: CAB49895.1.
HE613800 Genomic DNA. Translation: CCE70393.1.
PIRiB75074.
RefSeqiWP_010868104.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB49895; CAB49895; PAB2364.
GeneIDi1496337.
KEGGipab:PAB2364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248286 Genomic DNA. Translation: CAB49895.1.
HE613800 Genomic DNA. Translation: CCE70393.1.
PIRiB75074.
RefSeqiWP_010868104.1. NC_000868.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MKHX-ray2.01A616-722[»]
1RQGX-ray2.90A1-722[»]
ProteinModelPortaliQ9V011.
SMRiQ9V011. Positions 1-606, 616-722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272844.PAB2364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB49895; CAB49895; PAB2364.
GeneIDi1496337.
KEGGipab:PAB2364.

Phylogenomic databases

eggNOGiarCOG00810. Archaea.
COG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200402.
KOiK01874.
OMAiFWPAMLD.

Enzyme and pathway databases

BRENDAi6.1.1.10. 5242.

Miscellaneous databases

EvolutionaryTraceiQ9V011.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR004495. Met-tRNA-synth_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_PYRAB
AccessioniPrimary (citable) accession number: Q9V011
Secondary accession number(s): G8ZIF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.