Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methionine--tRNA ligase

Gene

metG

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi143ZincUniRule annotation1
Metal bindingi146ZincUniRule annotation1
Metal bindingi156ZincUniRule annotation1
Metal bindingi159ZincUniRule annotation1
Binding sitei347ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BRENDAi6.1.1.10. 5242.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotation
Synonyms:metS
Ordered Locus Names:PYRAB09870
ORF Names:PAB2364
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001391941 – 722Methionine--tRNA ligaseAdd BLAST722

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272844.PAB2364.

Structurei

Secondary structure

1722
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi19 – 25Combined sources7
Helixi27 – 38Combined sources12
Beta strandi42 – 50Combined sources9
Helixi54 – 63Combined sources10
Helixi67 – 85Combined sources19
Beta strandi90 – 94Combined sources5
Helixi98 – 113Combined sources16
Beta strandi117 – 127Combined sources11
Turni128 – 131Combined sources4
Helixi136 – 138Combined sources3
Beta strandi144 – 146Combined sources3
Turni153 – 155Combined sources3
Beta strandi157 – 159Combined sources3
Beta strandi167 – 170Combined sources4
Turni174 – 176Combined sources3
Beta strandi181 – 190Combined sources10
Helixi192 – 194Combined sources3
Helixi196 – 204Combined sources9
Helixi210 – 220Combined sources11
Beta strandi234 – 236Combined sources3
Beta strandi244 – 246Combined sources3
Helixi253 – 256Combined sources4
Helixi257 – 259Combined sources3
Helixi260 – 271Combined sources12
Turni275 – 278Combined sources4
Helixi279 – 282Combined sources4
Beta strandi289 – 296Combined sources8
Helixi297 – 299Combined sources3
Helixi300 – 304Combined sources5
Helixi306 – 312Combined sources7
Beta strandi319 – 321Combined sources3
Beta strandi338 – 340Combined sources3
Turni347 – 350Combined sources4
Helixi355 – 358Combined sources4
Turni359 – 361Combined sources3
Helixi364 – 373Combined sources10
Beta strandi377 – 379Combined sources3
Beta strandi381 – 383Combined sources3
Helixi385 – 394Combined sources10
Helixi395 – 399Combined sources5
Helixi400 – 413Combined sources14
Helixi427 – 448Combined sources22
Helixi452 – 471Combined sources20
Helixi475 – 478Combined sources4
Turni479 – 481Combined sources3
Helixi483 – 504Combined sources22
Turni505 – 507Combined sources3
Helixi509 – 518Combined sources10
Helixi550 – 559Combined sources10
Turni560 – 564Combined sources5
Helixi566 – 576Combined sources11
Helixi579 – 590Combined sources12
Helixi591 – 594Combined sources4
Helixi595 – 601Combined sources7
Helixi620 – 624Combined sources5
Beta strandi628 – 638Combined sources11
Beta strandi646 – 651Combined sources6
Beta strandi656 – 661Combined sources6
Turni664 – 666Combined sources3
Helixi669 – 672Combined sources4
Beta strandi676 – 680Combined sources5
Beta strandi699 – 701Combined sources3
Beta strandi706 – 708Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MKHX-ray2.01A616-722[»]
1RQGX-ray2.90A1-722[»]
ProteinModelPortaliQ9V011.
SMRiQ9V011.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9V011.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini622 – 722tRNA-bindingUniRule annotationAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionAdd BLAST11
Motifi344 – 348"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00810. Archaea.
COG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200402.
KOiK01874.
OMAiFWPAMLD.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR004495. Met-tRNA-synth_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9V011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRYMVTSAL PYANGPIHAG HLAGAYLPAD IFVRYLRLKG EDVVFICGTD
60 70 80 90 100
EHGTPISFRA LKEGRSPREI VDEFHEQIKI TFQRAKISFD FFGRTELPIH
110 120 130 140 150
YKLSQEFFLK AYENGHLVKK VTKQAYCEHD KMFLPDRFVI GTCPYCGAED
160 170 180 190 200
QKGDQCEVCG RPLTPEILIN PRCAICGRPI SFRDSAHYYI KMQDFAERLK
210 220 230 240 250
RWIEKQPWKP NVKNMVLSWI EEGLEERAIT RDLNWGIPVP LDEEDMKGKV
260 270 280 290 300
LYVWFEAPIG YISITIEHFK RIGKPNEWKK YWLNIDGQTR VIHFIGKDNI
310 320 330 340 350
PFHAIFWPAF LMAYGKYKDE EVEAEWNLPY DIPANEYLTL EGKKFSTSRN
360 370 380 390 400
WAIWVHEFLD VFPADYLRYY LTTIMPETRD SDFSFSDFKV RINEELVNNL
410 420 430 440 450
GNFVHRALTF VNRYFDGVVP ERGELDELDR EALEEIEKAF KEVGELIMNY
460 470 480 490 500
RFKDALKRVM SLASFGNRYF DHKQPWKTAK EDKVRTGTTV NISLQIVKAL
510 520 530 540 550
GILLEPFLPD ASEKIWHLLN LDEVKRWEFR ELPAGHKVRK PEILFKKVTD
560 570 580 590 600
DQIIYFILNY MAKGNPEGAR ILLDKYYKRE DVIRVAKEKF GDEAEVVLRR
610 620 630 640 650
VYKDIKLKEK KEGKEMYVKF DDFAKLDLRV GKIIEVKDHP NADKLYVVKV
660 670 680 690 700
DLGDEVRTLV AGLKKYYKPE ELLNRYVVVV ANLEPKKLRG IGSQGMLLAA
710 720
DDGERVALLM PDKEVKLGAK VR
Length:722
Mass (Da):84,295
Last modified:May 1, 2000 - v1
Checksum:iA69078A91D5BE028
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248286 Genomic DNA. Translation: CAB49895.1.
HE613800 Genomic DNA. Translation: CCE70393.1.
PIRiB75074.
RefSeqiWP_010868104.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB49895; CAB49895; PAB2364.
GeneIDi1496337.
KEGGipab:PAB2364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248286 Genomic DNA. Translation: CAB49895.1.
HE613800 Genomic DNA. Translation: CCE70393.1.
PIRiB75074.
RefSeqiWP_010868104.1. NC_000868.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MKHX-ray2.01A616-722[»]
1RQGX-ray2.90A1-722[»]
ProteinModelPortaliQ9V011.
SMRiQ9V011.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272844.PAB2364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB49895; CAB49895; PAB2364.
GeneIDi1496337.
KEGGipab:PAB2364.

Phylogenomic databases

eggNOGiarCOG00810. Archaea.
COG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200402.
KOiK01874.
OMAiFWPAMLD.

Enzyme and pathway databases

BRENDAi6.1.1.10. 5242.

Miscellaneous databases

EvolutionaryTraceiQ9V011.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR004495. Met-tRNA-synth_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_PYRAB
AccessioniPrimary (citable) accession number: Q9V011
Secondary accession number(s): G8ZIF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.