Q9UZV7 (KHSE_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	PYRAB10390
ORF Names:	PAB1676

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Homoserine kinase HAMAP MF_00384		PRO_0000156644
Regions
	Nucleotide binding	83 – 93	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9UZV7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B4BD92823F7E63FE	FASTA	294	31,640
	10 20 30 40 50 60 MKKRIYAPAT IANFGPGFDV FGMAIEEPGD EVIVKESDSF EIEVEGYDVP RDENNVAVIS 70 80 90 100 110 120 AKALFKMVGE EGGVKIRLKK GVRPKSGLGS SGASSVAGAL AAARVLGVDN DELIIMAALE 130 140 150 160 170 180 GEKAASGSPH GDNVIPSYYG GFNILESLNP LRVHRVDVEL NVVVVLPEVE VPTKEARRIV 190 200 210 220 230 240 PEKVPLKDAI KNLAMASSLV LALKEGDIET VGRLLDDNLA LPYRKKLMPW FDEVRKAGLE 250 260 270 280 290 AGAYGVTVSG SGPSLFAIGE NLKDIGKAMK EKFEELGIRA EFWITKTGRG AKWY

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248286 Genomic DNA. Translation: CAB49949.1.
PIR	H75080.
RefSeq	NP_126718.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9UZV7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000003292; EBPYRP00000003223; EBPYRG00000003292.
GeneID	1496393.
GenomeReviews	Gene locus PYRAB10390 in contig AL096836_GR.
KEGG	pab:PAB1676.
NMPDR	fig|272844.1.peg.1086.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022725.
HOGENOM	HBG646290.
OMA	GSAHADN.
PhylomeDB	Q9UZV7.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	PABY272844:PAB1676-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_PYRAB

Accession

Primary (citable) accession number: Q9UZV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program