Q9UZQ4 (THIM_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	PYRAB10910
ORF Names:	PAB2432

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Magnesium By similarity. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000156970
Sites
	Binding site	46	1	Substrate; via amide nitrogen By similarity
	Binding site	121	1	ATP By similarity
	Binding site	168	1	ATP By similarity
	Binding site	195	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UZQ4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 659156D1F9180863	FASTA	268	29,004
	10 20 30 40 50 60 MNALNFIVEA LNEVRKRKPL VHNITNFVVM NTTANALLAL GASPVMAHAE EELEEMIGLA 70 80 90 100 110 120 DAVVINIGTL DSWWRKSMIK AVEIANEMKK PIVLDPVGAG ATKLRTKVAL EILDRGVTVL 130 140 150 160 170 180 KGNFGEISSL LGEFGKTRGV DSSEYDEEKA KVLALSAAKE FNTTVAVTGA VDYVSDGGKV 190 200 210 220 230 240 FAIYNGHKLL ERVTGTGCIV TAITGAFVAV TEPLRAAISS LVVFGIAAEK AYEEAKYPGS 250 260 FHVKLYDWLY RIDGEVIRKY AKVREVGV

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248286 Genomic DNA. Translation: CAB50002.1.
PIR	E75087.
RefSeq	NP_126771.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9UZQ4.
SMR	Q9UZQ4. Positions 4-266.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000003583; EBPYRP00000003514; EBPYRG00000003583.
GeneID	1496449.
GenomeReviews	Gene locus PYRAB10910 in contig AL096836_GR.
KEGG	pab:PAB2432.
NMPDR	fig|272844.1.peg.1142.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022541.
HOGENOM	HBG351126.
OMA	AIRGNAG.
PhylomeDB	Q9UZQ4.
ProtClustDB	PRK09355.
Enzyme and pathway databases
BioCyc	PABY272844:PAB2432-MONOMER.
Family and domain databases
HAMAP	MF_00228. Thz_kinase. [Tree]
InterPro	IPR000417. Hyethyz_kinase. [Graphical view]
KO	K00878.
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. ThiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_PYRAB

Accession

Primary (citable) accession number: Q9UZQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program