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Q9UZQ4 (THIM_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:PYRAB10910
ORF Names:PAB2432
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence caution

The sequence CAB50002.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000156970

Sites

Binding site431Substrate; via amide nitrogen By similarity
Binding site1181ATP By similarity
Binding site1651ATP By similarity
Binding site1921Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9UZQ4 [UniParc].

Last modified May 16, 2012. Version 2.
Checksum: 5E7C4084BA3C5A18

FASTA26528,705
        10         20         30         40         50         60 
MNFIVEALNE VRKRKPLVHN ITNFVVMNTT ANALLALGAS PVMAHAEEEL EEMIGLADAV 

        70         80         90        100        110        120 
VINIGTLDSW WRKSMIKAVE IANEMKKPIV LDPVGAGATK LRTKVALEIL DRGVTVLKGN 

       130        140        150        160        170        180 
FGEISSLLGE FGKTRGVDSS EYDEEKAKVL ALSAAKEFNT TVAVTGAVDY VSDGGKVFAI 

       190        200        210        220        230        240 
YNGHKLLERV TGTGCIVTAI TGAFVAVTEP LRAAISSLVV FGIAAEKAYE EAKYPGSFHV 

       250        260 
KLYDWLYRID GEVIRKYAKV REVGV 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248286 Genomic DNA. Translation: CAB50002.1. Different initiation.
HE613800 Genomic DNA. Translation: CCE70504.1.
PIRE75087.
RefSeqNP_126771.1. NC_000868.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272844.PAB2432.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB50002; CAB50002; PAB2432.
GeneID1496449.
KEGGpab:PAB2432.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
ProtClustDBPRK09355.

Enzyme and pathway databases

UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_PYRAB
AccessionPrimary (citable) accession number: Q9UZQ4
Secondary accession number(s): G8ZJP5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 16, 2012
Last modified: February 19, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways