ID   IF2P_PYRAB              Reviewed;         992 AA.
AC   Q9UZK7; G8ZKB1;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   27-MAR-2024, entry version 146.
DE   RecName: Full=Probable translation initiation factor IF-2;
DE   Contains:
DE     RecName: Full=Pab infB intein;
DE     AltName: Full=Pab IF2 intein;
GN   Name=infB; OrderedLocusNames=PYRAB11390; ORFNames=PAB0755;
OS   Pyrococcus abyssi (strain GE5 / Orsay).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=272844;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GE5 / Orsay;
RX   PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA   Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA   Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA   Weissenbach J., Zivanovic Y., Forterre P.;
RT   "An integrated analysis of the genome of the hyperthermophilic archaeon
RT   Pyrococcus abyssi.";
RL   Mol. Microbiol. 47:1495-1512(2003).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=GE5 / Orsay;
RX   PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA   Gao J., Wang J.;
RT   "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT   Pyrococcus furiosus DSM 3638.";
RL   Curr. Microbiol. 64:118-129(2012).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2 (By similarity). {ECO:0000250}.
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
CC   -!- MISCELLANEOUS: The intein interrupts the GTP-binding site.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AJ248286; CAB50050.1; -; Genomic_DNA.
DR   EMBL; HE613800; CCE70554.1; -; Genomic_DNA.
DR   PIR; E75093; E75093.
DR   PDB; 7YYP; X-ray; 2.90 A; A=414-992.
DR   PDB; 7YZN; X-ray; 1.70 A; A=414-852.
DR   PDB; 7ZAG; EM; 2.77 A; 7=414-992.
DR   PDB; 7ZAH; EM; 2.70 A; 7=414-992.
DR   PDB; 7ZKI; EM; 3.60 A; 7=418-992.
DR   PDBsum; 7YYP; -.
DR   PDBsum; 7YZN; -.
DR   PDBsum; 7ZAG; -.
DR   PDBsum; 7ZAH; -.
DR   PDBsum; 7ZKI; -.
DR   AlphaFoldDB; Q9UZK7; -.
DR   EMDB; EMD-14579; -.
DR   EMDB; EMD-14580; -.
DR   EMDB; EMD-14763; -.
DR   SMR; Q9UZK7; -.
DR   STRING; 272844.PAB0755; -.
DR   MEROPS; N10.004; -.
DR   KEGG; pab:PAB0755; -.
DR   PATRIC; fig|272844.11.peg.1195; -.
DR   eggNOG; arCOG01560; Archaea.
DR   eggNOG; arCOG03151; Archaea.
DR   HOGENOM; CLU_002656_3_4_2; -.
DR   OrthoDB; 30957at2157; -.
DR   PhylomeDB; Q9UZK7; -.
DR   Proteomes; UP000000810; Chromosome.
DR   Proteomes; UP000009139; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   CDD; cd03703; aeIF5B_II; 1.
DR   CDD; cd00081; Hint; 2.
DR   CDD; cd16266; IF2_aeIF5B_IV; 1.
DR   CDD; cd01887; IF2_eIF5B; 1.
DR   Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR   Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   Gene3D; 2.40.30.10; Translation factors; 2.
DR   Gene3D; 3.40.50.10050; Translation initiation factor IF- 2, domain 3; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR004042; Intein_endonuc.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   NCBIfam; TIGR00491; aIF-2; 1.
DR   NCBIfam; TIGR01443; intein_Cterm; 1.
DR   NCBIfam; TIGR01445; intein_Nterm; 1.
DR   NCBIfam; TIGR00231; small_GTP; 1.
DR   PANTHER; PTHR43381:SF4; EUKARYOTIC TRANSLATION INITIATION FACTOR 5B; 1.
DR   PANTHER; PTHR43381; TRANSLATION INITIATION FACTOR IF-2-RELATED; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   Pfam; PF14890; Intein_splicing; 1.
DR   SMART; SM00305; HintC; 1.
DR   SMART; SM00306; HintN; 1.
DR   SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR   SUPFAM; SSF52156; Initiation factor IF2/eIF5b, domain 3; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   SUPFAM; SSF50447; Translation proteins; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Autocatalytic cleavage; Endonuclease; GTP-binding; Hydrolase;
KW   Initiation factor; Intron homing; Nuclease; Nucleotide-binding;
KW   Protein biosynthesis; Protein splicing.
FT   CHAIN           1..20
FT                   /note="Probable translation initiation factor IF-2, 1st
FT                   part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000014487"
FT   CHAIN           21..414
FT                   /note="Pab infB intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000014488"
FT   CHAIN           415..992
FT                   /note="Probable translation initiation factor IF-2, 2nd
FT                   part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000014489"
FT   DOMAIN          96..220
FT                   /note="DOD-type homing endonuclease"
FT   DOMAIN          399..616
FT                   /note="tr-type G"
FT   BINDING         472..476
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         526..529
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   HELIX           414..422
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           426..429
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           431..433
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          439..444
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           445..452
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           453..458
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          459..461
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          467..472
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           477..479
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           480..489
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          491..498
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   TURN            499..501
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           505..517
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          521..526
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           528..530
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          531..533
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   HELIX           542..545
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           546..548
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           551..570
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          574..577
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           578..580
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   TURN            584..586
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          587..592
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   TURN            595..598
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           601..619
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          624..626
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   STRAND          629..638
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   TURN            639..641
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          642..655
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          659..662
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          664..667
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          669..672
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          675..678
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          693..707
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           711..713
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          718..722
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           726..742
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          749..758
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           759..771
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          776..784
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           786..798
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           800..802
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          804..809
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           814..823
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   STRAND          826..832
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           833..848
FT                   /evidence="ECO:0007829|PDB:7YZN"
FT   HELIX           855..858
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   STRAND          863..867
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   STRAND          874..876
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   STRAND          878..890
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   STRAND          897..899
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   STRAND          905..917
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   STRAND          927..934
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   TURN            938..940
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   STRAND          946..949
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   HELIX           953..961
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   HELIX           969..982
FT                   /evidence="ECO:0007829|PDB:7ZAH"
FT   HELIX           987..991
FT                   /evidence="ECO:0007829|PDB:7ZAH"
SQ   SEQUENCE   992 AA;  112225 MW;  9838BADA50E6F1C6 CRC64;
     MTKRIRQPII AVLGHVDHGK CLLPDEKVVV PSVGFVTLKE LFETASKVVE RDDEKEIREL
     DERITSVNGD GKTGLVKASY VWKVRHKGKV IRVKLKNWHG VTVTPEHPFL TTKGWKRADQ
     LRPGDYVAVP RFIHGNEDEK IFLSYVKVKK SGEEWKEYFY LAGRKGNIDV NLLFVAPKRY
     VVEFLRGYFE ERSEVKGESV IVEARELVEP LSLALLRFGI FSKIQGSKLI VTGKRNLEAF
     KDYIGFKDER EKALEEAIEK VKGSEVYPIF EEIRRLRLLF GFTREELGSY AKYENSEAPT
     YEELMEILDF IERGSPSLSK KIAILEGKLK AELRVLEEEG LIKDGKLTPL GRELLEVWRN
     REFDSKDVDY IRNIAETLVF IPVENVEEEE YDGYVYDLTT ETHNFIANGI LVHNTTLLDR
     IRKTNVAAKE AGGITQHIGA TEVPIEVVKK IAGPLIKLWK AEIKLPGLLF IDTPGHEAFT
     SLRARGGSLA DLAVLVVDIN EGFQPQTIES IEILRKYRTP FVVAANKIDR IKGWVIEEDE
     PFLMNIKKQD QRAVQELETK LWELIGKFYE FGFQANRFDR VQNFTRELAI VPISAKYGIG
     IAELLVLIAG LSQRYLEEKL KIEVEGPARG TILEVREEPG LGHTIDVIIY DGTLHKDDTI
     VVGGKDKAIV TKIRALLKPK PLDEIRDPRF RFDYVDEVTA AAGVKIAAPG LEEALAGSPV
     IAAPTPEDVE KAKQEILEQI ERVVISTDKV GVIVKADTLG SLEALSKELQ EKEIPIRKAD
     VGNVSKTDVM EALSVKEEEP KYGVILGFNV KVNEDAEEVA KAKDVKIFVG NVIYKLIEDY
     EEWVKEEEEK KKRELLSKVT FPGVIRLYPD ERYVFRRSNP AIVGIEVIEG RIKPGVTLIK
     QNGQKVGVIR SIKSRDEFLQ EAKKGQAVAI AIEGAIVGRH IHPGETLYVD LSRDDAITLL
     KHLRDTLEDT DIKALKMIAK VKAKEDPFWR AI
//