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Q9UZK7

- IF2P_PYRAB

UniProt

Q9UZK7 - IF2P_PYRAB

Protein

Probable translation initiation factor IF-2

Gene

infB

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi472 – 4765GTPBy similarity
    Nucleotide bindingi526 – 5294GTPBy similarity

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. GTPase activity Source: InterPro
    3. GTP binding Source: UniProtKB-KW
    4. translation initiation factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. intein-mediated protein splicing Source: InterPro
    2. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Initiation factor, Nuclease

    Keywords - Biological processi

    Intron homing, Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiN10.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable translation initiation factor IF-2
    Cleaved into the following chain:
    Alternative name(s):
    Pab IF2 intein
    Gene namesi
    Name:infB
    Ordered Locus Names:PYRAB11390
    ORF Names:PAB0755
    OrganismiPyrococcus abyssi (strain GE5 / Orsay)
    Taxonomic identifieri272844 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000810: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2020Probable translation initiation factor IF-2, 1st partSequence AnalysisPRO_0000014487Add
    BLAST
    Chaini21 – 414394Pab infB inteinSequence AnalysisPRO_0000014488Add
    BLAST
    Chaini415 – 992578Probable translation initiation factor IF-2, 2nd partSequence AnalysisPRO_0000014489Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Proteomic databases

    PRIDEiQ9UZK7.

    Interactioni

    Protein-protein interaction databases

    STRINGi272844.PAB0755.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UZK7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini96 – 220125DOD-type homing endonucleaseAdd
    BLAST
    Domaini399 – 616218tr-type GAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0532.
    HOGENOMiHOG000105770.
    KOiK03243.
    OMAiDHGKCLL.

    Family and domain databases

    Gene3Di2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.10050. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00100_A. IF_2_A.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR029459. EFTU-type.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR004544. TIF_aIF-2_arc.
    IPR023115. TIF_IF2_dom3.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF14578. GTP_EFTU_D4. 1 hit.
    PF11987. IF-2. 1 hit.
    [Graphical view]
    SMARTiSM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52156. SSF52156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00491. aIF-2. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS51722. G_TR_2. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9UZK7-1 [UniParc]FASTAAdd to Basket

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    MTKRIRQPII AVLGHVDHGK CLLPDEKVVV PSVGFVTLKE LFETASKVVE    50
    RDDEKEIREL DERITSVNGD GKTGLVKASY VWKVRHKGKV IRVKLKNWHG 100
    VTVTPEHPFL TTKGWKRADQ LRPGDYVAVP RFIHGNEDEK IFLSYVKVKK 150
    SGEEWKEYFY LAGRKGNIDV NLLFVAPKRY VVEFLRGYFE ERSEVKGESV 200
    IVEARELVEP LSLALLRFGI FSKIQGSKLI VTGKRNLEAF KDYIGFKDER 250
    EKALEEAIEK VKGSEVYPIF EEIRRLRLLF GFTREELGSY AKYENSEAPT 300
    YEELMEILDF IERGSPSLSK KIAILEGKLK AELRVLEEEG LIKDGKLTPL 350
    GRELLEVWRN REFDSKDVDY IRNIAETLVF IPVENVEEEE YDGYVYDLTT 400
    ETHNFIANGI LVHNTTLLDR IRKTNVAAKE AGGITQHIGA TEVPIEVVKK 450
    IAGPLIKLWK AEIKLPGLLF IDTPGHEAFT SLRARGGSLA DLAVLVVDIN 500
    EGFQPQTIES IEILRKYRTP FVVAANKIDR IKGWVIEEDE PFLMNIKKQD 550
    QRAVQELETK LWELIGKFYE FGFQANRFDR VQNFTRELAI VPISAKYGIG 600
    IAELLVLIAG LSQRYLEEKL KIEVEGPARG TILEVREEPG LGHTIDVIIY 650
    DGTLHKDDTI VVGGKDKAIV TKIRALLKPK PLDEIRDPRF RFDYVDEVTA 700
    AAGVKIAAPG LEEALAGSPV IAAPTPEDVE KAKQEILEQI ERVVISTDKV 750
    GVIVKADTLG SLEALSKELQ EKEIPIRKAD VGNVSKTDVM EALSVKEEEP 800
    KYGVILGFNV KVNEDAEEVA KAKDVKIFVG NVIYKLIEDY EEWVKEEEEK 850
    KKRELLSKVT FPGVIRLYPD ERYVFRRSNP AIVGIEVIEG RIKPGVTLIK 900
    QNGQKVGVIR SIKSRDEFLQ EAKKGQAVAI AIEGAIVGRH IHPGETLYVD 950
    LSRDDAITLL KHLRDTLEDT DIKALKMIAK VKAKEDPFWR AI 992
    Length:992
    Mass (Da):112,225
    Last modified:May 1, 2000 - v1
    Checksum:i9838BADA50E6F1C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ248286 Genomic DNA. Translation: CAB50050.1.
    HE613800 Genomic DNA. Translation: CCE70554.1.
    PIRiE75093.
    RefSeqiNP_126820.1. NC_000868.1.

    Genome annotation databases

    EnsemblBacteriaiCAB50050; CAB50050; PAB0755.
    GeneIDi1496500.
    KEGGipab:PAB0755.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ248286 Genomic DNA. Translation: CAB50050.1 .
    HE613800 Genomic DNA. Translation: CCE70554.1 .
    PIRi E75093.
    RefSeqi NP_126820.1. NC_000868.1.

    3D structure databases

    ProteinModelPortali Q9UZK7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272844.PAB0755.

    Protein family/group databases

    MEROPSi N10.004.

    Proteomic databases

    PRIDEi Q9UZK7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB50050 ; CAB50050 ; PAB0755 .
    GeneIDi 1496500.
    KEGGi pab:PAB0755.

    Phylogenomic databases

    eggNOGi COG0532.
    HOGENOMi HOG000105770.
    KOi K03243.
    OMAi DHGKCLL.

    Family and domain databases

    Gene3Di 2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.10050. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00100_A. IF_2_A.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR029459. EFTU-type.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR004544. TIF_aIF-2_arc.
    IPR023115. TIF_IF2_dom3.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF14578. GTP_EFTU_D4. 1 hit.
    PF11987. IF-2. 1 hit.
    [Graphical view ]
    SMARTi SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52156. SSF52156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00491. aIF-2. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51722. G_TR_2. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GE5 / Orsay.
    2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
      Gao J., Wang J.
      Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: GE5 / Orsay.

    Entry informationi

    Entry nameiIF2P_PYRAB
    AccessioniPrimary (citable) accession number: Q9UZK7
    Secondary accession number(s): G8ZKB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The intein interrupts the GTP-binding site.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3