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Protein

Probable translation initiation factor IF-2

Gene

infB

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity).By similarity

Miscellaneous

The intein interrupts the GTP-binding site.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi472 – 476GTPBy similarity5
Nucleotide bindingi526 – 529GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Initiation factor, Nuclease
Biological processIntron homing, Protein biosynthesis
LigandGTP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiN10.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable translation initiation factor IF-2
Cleaved into the following chain:
Alternative name(s):
Pab IF2 intein
Gene namesi
Name:infB
Ordered Locus Names:PYRAB11390
ORF Names:PAB0755
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000144871 – 20Probable translation initiation factor IF-2, 1st partSequence analysisAdd BLAST20
ChainiPRO_000001448821 – 414Pab infB inteinSequence analysisAdd BLAST394
ChainiPRO_0000014489415 – 992Probable translation initiation factor IF-2, 2nd partSequence analysisAdd BLAST578

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiQ9UZK7.

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB0755.

Structurei

3D structure databases

ProteinModelPortaliQ9UZK7.
SMRiQ9UZK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini96 – 220DOD-type homing endonucleaseAdd BLAST125
Domaini399 – 616tr-type GAdd BLAST218

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01560. Archaea.
COG0532. LUCA.
COG1372. LUCA.
HOGENOMiHOG000105770.
KOiK03243.
OMAiDHGKCLL.
OrthoDBiPOG093Z012A.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
HAMAPiMF_00100_A. IF_2_A. 1 hit.
InterProiView protein in InterPro
IPR004161. EFTu-like_2.
IPR029459. EFTU-type.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
PfamiView protein in Pfam
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
SMARTiView protein in SMART
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UZK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKRIRQPII AVLGHVDHGK CLLPDEKVVV PSVGFVTLKE LFETASKVVE
60 70 80 90 100
RDDEKEIREL DERITSVNGD GKTGLVKASY VWKVRHKGKV IRVKLKNWHG
110 120 130 140 150
VTVTPEHPFL TTKGWKRADQ LRPGDYVAVP RFIHGNEDEK IFLSYVKVKK
160 170 180 190 200
SGEEWKEYFY LAGRKGNIDV NLLFVAPKRY VVEFLRGYFE ERSEVKGESV
210 220 230 240 250
IVEARELVEP LSLALLRFGI FSKIQGSKLI VTGKRNLEAF KDYIGFKDER
260 270 280 290 300
EKALEEAIEK VKGSEVYPIF EEIRRLRLLF GFTREELGSY AKYENSEAPT
310 320 330 340 350
YEELMEILDF IERGSPSLSK KIAILEGKLK AELRVLEEEG LIKDGKLTPL
360 370 380 390 400
GRELLEVWRN REFDSKDVDY IRNIAETLVF IPVENVEEEE YDGYVYDLTT
410 420 430 440 450
ETHNFIANGI LVHNTTLLDR IRKTNVAAKE AGGITQHIGA TEVPIEVVKK
460 470 480 490 500
IAGPLIKLWK AEIKLPGLLF IDTPGHEAFT SLRARGGSLA DLAVLVVDIN
510 520 530 540 550
EGFQPQTIES IEILRKYRTP FVVAANKIDR IKGWVIEEDE PFLMNIKKQD
560 570 580 590 600
QRAVQELETK LWELIGKFYE FGFQANRFDR VQNFTRELAI VPISAKYGIG
610 620 630 640 650
IAELLVLIAG LSQRYLEEKL KIEVEGPARG TILEVREEPG LGHTIDVIIY
660 670 680 690 700
DGTLHKDDTI VVGGKDKAIV TKIRALLKPK PLDEIRDPRF RFDYVDEVTA
710 720 730 740 750
AAGVKIAAPG LEEALAGSPV IAAPTPEDVE KAKQEILEQI ERVVISTDKV
760 770 780 790 800
GVIVKADTLG SLEALSKELQ EKEIPIRKAD VGNVSKTDVM EALSVKEEEP
810 820 830 840 850
KYGVILGFNV KVNEDAEEVA KAKDVKIFVG NVIYKLIEDY EEWVKEEEEK
860 870 880 890 900
KKRELLSKVT FPGVIRLYPD ERYVFRRSNP AIVGIEVIEG RIKPGVTLIK
910 920 930 940 950
QNGQKVGVIR SIKSRDEFLQ EAKKGQAVAI AIEGAIVGRH IHPGETLYVD
960 970 980 990
LSRDDAITLL KHLRDTLEDT DIKALKMIAK VKAKEDPFWR AI
Length:992
Mass (Da):112,225
Last modified:May 1, 2000 - v1
Checksum:i9838BADA50E6F1C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248286 Genomic DNA. Translation: CAB50050.1.
HE613800 Genomic DNA. Translation: CCE70554.1.
PIRiE75093.

Genome annotation databases

EnsemblBacteriaiCAB50050; CAB50050; PAB0755.
KEGGipab:PAB0755.
PATRICifig|272844.11.peg.1195.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIF2P_PYRAB
AccessioniPrimary (citable) accession number: Q9UZK7
Secondary accession number(s): G8ZKB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: July 5, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families