Q9UZK7 (IF2P_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable translation initiation factor IF-2

Cleaved into the following chain:

1. Pab infB intein
   Alternative name(s):
   Pab IF2 intein

Gene names

Name:	infB
Ordered Locus Names:	PYRAB11390
ORF Names:	PAB0755

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus abyssi

Protein attributes

Sequence length	992 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity. HAMAP-Rule MF_00100_A
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00100_A
Miscellaneous	The intein interrupts the GTP-binding site. HAMAP-Rule MF_00100_A
Sequence similarities	Belongs to the IF-2 family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing Protein biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Initiation factor Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 20	20	Probable translation initiation factor IF-2, 1st part Potential		PRO_0000014487
	Chain	21 – 414	394	Pab infB intein Potential		PRO_0000014488
	Chain	415 – 992	578	Probable translation initiation factor IF-2, 2nd part Potential		PRO_0000014489
Regions
	Domain	96 – 220	125	DOD-type homing endonuclease
	Nucleotide binding	472 – 476	5	GTP By similarity
	Nucleotide binding	526 – 529	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UZK7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 9838BADA50E6F1C6	FASTA	992	112,225
	10 20 30 40 50 60 MTKRIRQPII AVLGHVDHGK CLLPDEKVVV PSVGFVTLKE LFETASKVVE RDDEKEIREL 70 80 90 100 110 120 DERITSVNGD GKTGLVKASY VWKVRHKGKV IRVKLKNWHG VTVTPEHPFL TTKGWKRADQ 130 140 150 160 170 180 LRPGDYVAVP RFIHGNEDEK IFLSYVKVKK SGEEWKEYFY LAGRKGNIDV NLLFVAPKRY 190 200 210 220 230 240 VVEFLRGYFE ERSEVKGESV IVEARELVEP LSLALLRFGI FSKIQGSKLI VTGKRNLEAF 250 260 270 280 290 300 KDYIGFKDER EKALEEAIEK VKGSEVYPIF EEIRRLRLLF GFTREELGSY AKYENSEAPT 310 320 330 340 350 360 YEELMEILDF IERGSPSLSK KIAILEGKLK AELRVLEEEG LIKDGKLTPL GRELLEVWRN 370 380 390 400 410 420 REFDSKDVDY IRNIAETLVF IPVENVEEEE YDGYVYDLTT ETHNFIANGI LVHNTTLLDR 430 440 450 460 470 480 IRKTNVAAKE AGGITQHIGA TEVPIEVVKK IAGPLIKLWK AEIKLPGLLF IDTPGHEAFT 490 500 510 520 530 540 SLRARGGSLA DLAVLVVDIN EGFQPQTIES IEILRKYRTP FVVAANKIDR IKGWVIEEDE 550 560 570 580 590 600 PFLMNIKKQD QRAVQELETK LWELIGKFYE FGFQANRFDR VQNFTRELAI VPISAKYGIG 610 620 630 640 650 660 IAELLVLIAG LSQRYLEEKL KIEVEGPARG TILEVREEPG LGHTIDVIIY DGTLHKDDTI 670 680 690 700 710 720 VVGGKDKAIV TKIRALLKPK PLDEIRDPRF RFDYVDEVTA AAGVKIAAPG LEEALAGSPV 730 740 750 760 770 780 IAAPTPEDVE KAKQEILEQI ERVVISTDKV GVIVKADTLG SLEALSKELQ EKEIPIRKAD 790 800 810 820 830 840 VGNVSKTDVM EALSVKEEEP KYGVILGFNV KVNEDAEEVA KAKDVKIFVG NVIYKLIEDY 850 860 870 880 890 900 EEWVKEEEEK KKRELLSKVT FPGVIRLYPD ERYVFRRSNP AIVGIEVIEG RIKPGVTLIK 910 920 930 940 950 960 QNGQKVGVIR SIKSRDEFLQ EAKKGQAVAI AIEGAIVGRH IHPGETLYVD LSRDDAITLL 970 980 990 KHLRDTLEDT DIKALKMIAK VKAKEDPFWR AI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.
[2]	"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638." Gao J., Wang J. Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248286 Genomic DNA. Translation: CAB50050.1. HE613800 Genomic DNA. Translation: CCE70554.1.
PIR	E75093.
RefSeq	NP_126820.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9UZK7.
ModBase	Search...
Protein-protein interaction databases
STRING	272844.PAB0755.
Protein family/group databases
MEROPS	N10.004.
Proteomic databases
PRIDE	Q9UZK7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB50050; CAB50050; PAB0755.
GeneID	1496500.
KEGG	pab:PAB0755.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0532.
HOGENOM	HOG000105770.
KO	K03243.
OMA	EVWRNRE.
ProtClustDB	PRK14845.
Family and domain databases
Gene3D	3.10.28.10. 1 hit. 3.40.50.10050. 1 hit.
HAMAP	MF_00100_A. IF-2_A.
InterPro	IPR000795. EF_GTP-bd_dom. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR027434. Homing_endonucl. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR004544. TIF_aIF-2_arc. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. [Graphical view]
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit. SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00491. aIF-2. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. False negative. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2P_PYRAB

Accession

Primary (citable) accession number: Q9UZK7
Secondary accession number(s): G8ZKB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program