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Q9UZK7

- IF2P_PYRAB

UniProt

Q9UZK7 - IF2P_PYRAB

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Protein

Probable translation initiation factor IF-2

Gene

infB

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi472 – 4765GTPBy similarity
Nucleotide bindingi526 – 5294GTPBy similarity

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. GTPase activity Source: InterPro
  3. GTP binding Source: UniProtKB-KW
  4. translation initiation factor activity Source: UniProtKB-KW

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Initiation factor, Nuclease

Keywords - Biological processi

Intron homing, Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable translation initiation factor IF-2
Cleaved into the following chain:
Alternative name(s):
Pab IF2 intein
Gene namesi
Name:infB
Ordered Locus Names:PYRAB11390
ORF Names:PAB0755
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000810: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2020Probable translation initiation factor IF-2, 1st partSequence AnalysisPRO_0000014487Add
BLAST
Chaini21 – 414394Pab infB inteinSequence AnalysisPRO_0000014488Add
BLAST
Chaini415 – 992578Probable translation initiation factor IF-2, 2nd partSequence AnalysisPRO_0000014489Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiQ9UZK7.

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB0755.

Structurei

3D structure databases

ProteinModelPortaliQ9UZK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini96 – 220125DOD-type homing endonucleaseAdd
BLAST
Domaini399 – 616218tr-type GAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0532.
HOGENOMiHOG000105770.
KOiK03243.
OMAiDHGKCLL.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_A. IF_2_A.
InterProiIPR000795. EF_GTP-bd_dom.
IPR029459. EFTU-type.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view]
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UZK7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKRIRQPII AVLGHVDHGK CLLPDEKVVV PSVGFVTLKE LFETASKVVE
60 70 80 90 100
RDDEKEIREL DERITSVNGD GKTGLVKASY VWKVRHKGKV IRVKLKNWHG
110 120 130 140 150
VTVTPEHPFL TTKGWKRADQ LRPGDYVAVP RFIHGNEDEK IFLSYVKVKK
160 170 180 190 200
SGEEWKEYFY LAGRKGNIDV NLLFVAPKRY VVEFLRGYFE ERSEVKGESV
210 220 230 240 250
IVEARELVEP LSLALLRFGI FSKIQGSKLI VTGKRNLEAF KDYIGFKDER
260 270 280 290 300
EKALEEAIEK VKGSEVYPIF EEIRRLRLLF GFTREELGSY AKYENSEAPT
310 320 330 340 350
YEELMEILDF IERGSPSLSK KIAILEGKLK AELRVLEEEG LIKDGKLTPL
360 370 380 390 400
GRELLEVWRN REFDSKDVDY IRNIAETLVF IPVENVEEEE YDGYVYDLTT
410 420 430 440 450
ETHNFIANGI LVHNTTLLDR IRKTNVAAKE AGGITQHIGA TEVPIEVVKK
460 470 480 490 500
IAGPLIKLWK AEIKLPGLLF IDTPGHEAFT SLRARGGSLA DLAVLVVDIN
510 520 530 540 550
EGFQPQTIES IEILRKYRTP FVVAANKIDR IKGWVIEEDE PFLMNIKKQD
560 570 580 590 600
QRAVQELETK LWELIGKFYE FGFQANRFDR VQNFTRELAI VPISAKYGIG
610 620 630 640 650
IAELLVLIAG LSQRYLEEKL KIEVEGPARG TILEVREEPG LGHTIDVIIY
660 670 680 690 700
DGTLHKDDTI VVGGKDKAIV TKIRALLKPK PLDEIRDPRF RFDYVDEVTA
710 720 730 740 750
AAGVKIAAPG LEEALAGSPV IAAPTPEDVE KAKQEILEQI ERVVISTDKV
760 770 780 790 800
GVIVKADTLG SLEALSKELQ EKEIPIRKAD VGNVSKTDVM EALSVKEEEP
810 820 830 840 850
KYGVILGFNV KVNEDAEEVA KAKDVKIFVG NVIYKLIEDY EEWVKEEEEK
860 870 880 890 900
KKRELLSKVT FPGVIRLYPD ERYVFRRSNP AIVGIEVIEG RIKPGVTLIK
910 920 930 940 950
QNGQKVGVIR SIKSRDEFLQ EAKKGQAVAI AIEGAIVGRH IHPGETLYVD
960 970 980 990
LSRDDAITLL KHLRDTLEDT DIKALKMIAK VKAKEDPFWR AI
Length:992
Mass (Da):112,225
Last modified:May 1, 2000 - v1
Checksum:i9838BADA50E6F1C6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ248286 Genomic DNA. Translation: CAB50050.1.
HE613800 Genomic DNA. Translation: CCE70554.1.
PIRiE75093.
RefSeqiNP_126820.1. NC_000868.1.
WP_010868256.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB50050; CAB50050; PAB0755.
GeneIDi1496500.
KEGGipab:PAB0755.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ248286 Genomic DNA. Translation: CAB50050.1 .
HE613800 Genomic DNA. Translation: CCE70554.1 .
PIRi E75093.
RefSeqi NP_126820.1. NC_000868.1.
WP_010868256.1. NC_000868.1.

3D structure databases

ProteinModelPortali Q9UZK7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272844.PAB0755.

Proteomic databases

PRIDEi Q9UZK7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB50050 ; CAB50050 ; PAB0755 .
GeneIDi 1496500.
KEGGi pab:PAB0755.

Phylogenomic databases

eggNOGi COG0532.
HOGENOMi HOG000105770.
KOi K03243.
OMAi DHGKCLL.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00100_A. IF_2_A.
InterProi IPR000795. EF_GTP-bd_dom.
IPR029459. EFTU-type.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view ]
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiIF2P_PYRAB
AccessioniPrimary (citable) accession number: Q9UZK7
Secondary accession number(s): G8ZKB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the GTP-binding site.

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3