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Q9UZK7 (IF2P_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable translation initiation factor IF-2

Cleaved into the following chain:

  1. Pab infB intein
    Alternative name(s):
    Pab IF2 intein
Gene names
Name:infB
Ordered Locus Names:PYRAB11390
ORF Names:PAB0755
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length992 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity. HAMAP-Rule MF_00100_A

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00100_A

Miscellaneous

The intein interrupts the GTP-binding site.

Sequence similarities

Belongs to the IF-2 family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2020Probable translation initiation factor IF-2, 1st part Potential
PRO_0000014487
Chain21 – 414394Pab infB intein Potential
PRO_0000014488
Chain415 – 992578Probable translation initiation factor IF-2, 2nd part Potential
PRO_0000014489

Regions

Domain96 – 220125DOD-type homing endonuclease
Nucleotide binding472 – 4765GTP By similarity
Nucleotide binding526 – 5294GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9UZK7 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 9838BADA50E6F1C6

FASTA992112,225
        10         20         30         40         50         60 
MTKRIRQPII AVLGHVDHGK CLLPDEKVVV PSVGFVTLKE LFETASKVVE RDDEKEIREL 

        70         80         90        100        110        120 
DERITSVNGD GKTGLVKASY VWKVRHKGKV IRVKLKNWHG VTVTPEHPFL TTKGWKRADQ 

       130        140        150        160        170        180 
LRPGDYVAVP RFIHGNEDEK IFLSYVKVKK SGEEWKEYFY LAGRKGNIDV NLLFVAPKRY 

       190        200        210        220        230        240 
VVEFLRGYFE ERSEVKGESV IVEARELVEP LSLALLRFGI FSKIQGSKLI VTGKRNLEAF 

       250        260        270        280        290        300 
KDYIGFKDER EKALEEAIEK VKGSEVYPIF EEIRRLRLLF GFTREELGSY AKYENSEAPT 

       310        320        330        340        350        360 
YEELMEILDF IERGSPSLSK KIAILEGKLK AELRVLEEEG LIKDGKLTPL GRELLEVWRN 

       370        380        390        400        410        420 
REFDSKDVDY IRNIAETLVF IPVENVEEEE YDGYVYDLTT ETHNFIANGI LVHNTTLLDR 

       430        440        450        460        470        480 
IRKTNVAAKE AGGITQHIGA TEVPIEVVKK IAGPLIKLWK AEIKLPGLLF IDTPGHEAFT 

       490        500        510        520        530        540 
SLRARGGSLA DLAVLVVDIN EGFQPQTIES IEILRKYRTP FVVAANKIDR IKGWVIEEDE 

       550        560        570        580        590        600 
PFLMNIKKQD QRAVQELETK LWELIGKFYE FGFQANRFDR VQNFTRELAI VPISAKYGIG 

       610        620        630        640        650        660 
IAELLVLIAG LSQRYLEEKL KIEVEGPARG TILEVREEPG LGHTIDVIIY DGTLHKDDTI 

       670        680        690        700        710        720 
VVGGKDKAIV TKIRALLKPK PLDEIRDPRF RFDYVDEVTA AAGVKIAAPG LEEALAGSPV 

       730        740        750        760        770        780 
IAAPTPEDVE KAKQEILEQI ERVVISTDKV GVIVKADTLG SLEALSKELQ EKEIPIRKAD 

       790        800        810        820        830        840 
VGNVSKTDVM EALSVKEEEP KYGVILGFNV KVNEDAEEVA KAKDVKIFVG NVIYKLIEDY 

       850        860        870        880        890        900 
EEWVKEEEEK KKRELLSKVT FPGVIRLYPD ERYVFRRSNP AIVGIEVIEG RIKPGVTLIK 

       910        920        930        940        950        960 
QNGQKVGVIR SIKSRDEFLQ EAKKGQAVAI AIEGAIVGRH IHPGETLYVD LSRDDAITLL 

       970        980        990 
KHLRDTLEDT DIKALKMIAK VKAKEDPFWR AI 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248286 Genomic DNA. Translation: CAB50050.1.
HE613800 Genomic DNA. Translation: CCE70554.1.
PIRE75093.
RefSeqNP_126820.1. NC_000868.1.

3D structure databases

ProteinModelPortalQ9UZK7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272844.PAB0755.

Protein family/group databases

MEROPSN10.004.

Proteomic databases

PRIDEQ9UZK7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB50050; CAB50050; PAB0755.
GeneID1496500.
KEGGpab:PAB0755.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000105770.
KOK03243.
OMADHGKCLL.

Family and domain databases

Gene3D2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_A. IF_2_A.
InterProIPR000795. EF_GTP-bd_dom.
IPR029459. EFTU-type.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004544. TIF_aIF-2_arc.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14578. GTP_EFTU_D4. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view]
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00491. aIF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2P_PYRAB
AccessionPrimary (citable) accession number: Q9UZK7
Secondary accession number(s): G8ZKB1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries

Translation initiation factors

List of translation initiation factor entries