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Protein

23S rRNA (uracil(747)-C(5))-methyltransferase

Gene

PYRAB11450

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 5-methyl-uridine at position equivalent to 747 (m5U747) in 23S rRNA (m5U859 in the P.abyssi numbering).1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uracil(747) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil(747) in 23S rRNA.1 Publication

Enzyme regulationi

Activated by magnesium ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi67Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi70Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi137Iron-sulfur (4Fe-4S)By similarity1
Binding sitei253S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei279S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei300S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei341S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei367NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • rRNA methylation Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPABY272844:G1GT8-1242-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA (uracil(747)-C(5))-methyltransferase (EC:2.1.1.189)
Alternative name(s):
23S rRNA(m5U747)-methyltransferase
Gene namesi
Ordered Locus Names:PYRAB11450
ORF Names:PAB0760
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001620541 – 41023S rRNA (uracil(747)-C(5))-methyltransferaseAdd BLAST410

Proteomic databases

PRIDEiQ9UZK1

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB0760

Structurei

3D structure databases

ProteinModelPortaliQ9UZK1
SMRiQ9UZK1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG00123 Archaea
COG2265 LUCA
HOGENOMiHOG000029870
KOiK03212
OMAiACGGCKM
OrthoDBiPOG093Z0BJF

Family and domain databases

InterProiView protein in InterPro
IPR001566 23S_rRNA_MeTrfase_RlmD
IPR030390 MeTrfase_TrmA_AS
IPR030391 MeTrfase_TrmA_CS
IPR029063 SAM-dependent_MTases
IPR015985 TehB-like_dom
IPR010280 U5_MeTrfase_fam
PANTHERiPTHR11061 PTHR11061, 1 hit
PfamiView protein in Pfam
PF03848 TehB, 1 hit
PF05958 tRNA_U5-meth_tr, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00479 rumA, 1 hit
PROSITEiView protein in PROSITE
PS51687 SAM_MT_RNA_M5U, 1 hit
PS01230 TRMA_1, 1 hit
PS01231 TRMA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9UZK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGIIKGVSN DGLGVLGEVL VPFAYPGDVV EVISTRERFG RTIARDFKLV
60 70 80 90 100
KSSPIRVPGK CRYFGRCGGC LWQGLKYREQ LKLKEEIFKR VTGVEAEIKG
110 120 130 140 150
SPRIWFFRNI SNFIVTVNGI GFKEFGMPRT VVSVDECPVF SERTKLYIRA
160 170 180 190 200
MKRFLRETGL NPWNWKNGDV HYLQVREGKF TGEVMINVIA HIPPSGREEL
210 220 230 240 250
TEAFGFADSV YWSLKRDKRD DPKGIPTLIK GNEFIRESIE GLVYLIHPST
260 270 280 290 300
FFQTNSYALP ILLKAVESFA EGSKVLDLYS GVGTFSLYLA KKGFEVTGVE
310 320 330 340 350
VNEESVRVAK KSAEVNSLDV SFIPGRAEDA KLKGYETLIV DPPRKGLKDF
360 370 380 390 400
SKRIAKEGPE NLIYVSCNPS KFVLDYRNYL SKAYKIEDAV LIDMFPHTPH
410
VEAVVKLRRR
Length:410
Mass (Da):46,442
Last modified:May 1, 2000 - v1
Checksum:i5315A0A418E34024
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248286 Genomic DNA Translation: CAB50056.1
HE613800 Genomic DNA Translation: CCE70562.1
PIRiC75094
RefSeqiWP_010868262.1, NC_000868.1

Genome annotation databases

EnsemblBacteriaiCAB50056; CAB50056; PAB0760
GeneIDi1496508
KEGGipab:PAB0760
PATRICifig|272844.11.peg.1203

Similar proteinsi

Entry informationi

Entry nameiARLMC_PYRAB
AccessioniPrimary (citable) accession number: Q9UZK1
Secondary accession number(s): G8ZKB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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