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Protein

23S rRNA (uracil(747)-C(5))-methyltransferase

Gene

PYRAB11450

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 5-methyl-uridine at position equivalent to 747 (m5U747) in 23S rRNA (m5U859 in the P.abyssi numbering).1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uracil(747) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil(747) in 23S rRNA.1 Publication

Enzyme regulationi

Activated by magnesium ions.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur (4Fe-4S)By similarity
Metal bindingi67 – 671Iron-sulfur (4Fe-4S)By similarity
Metal bindingi70 – 701Iron-sulfur (4Fe-4S)By similarity
Metal bindingi137 – 1371Iron-sulfur (4Fe-4S)By similarity
Binding sitei253 – 2531S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei279 – 2791S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei300 – 3001S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei341 – 3411S-adenosyl-L-methioninePROSITE-ProRule annotation
Active sitei367 – 3671NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • rRNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA (uracil(747)-C(5))-methyltransferase (EC:2.1.1.189)
Alternative name(s):
23S rRNA(m5U747)-methyltransferase
Gene namesi
Ordered Locus Names:PYRAB11450
ORF Names:PAB0760
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 41041023S rRNA (uracil(747)-C(5))-methyltransferasePRO_0000162054Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB0760.

Structurei

3D structure databases

ProteinModelPortaliQ9UZK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG00123. Archaea.
COG2265. LUCA.
HOGENOMiHOG000029870.
OMAiQTNSYAL.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR001566. 23S_rRNA_MeTrfase_RlmD.
IPR030390. MeTrfase_TrmA_AS.
IPR030391. MeTrfase_TrmA_CS.
IPR012340. NA-bd_OB-fold.
IPR029063. SAM-dependent_MTases.
IPR015985. TehB-like_dom.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF03848. TehB. 1 hit.
PF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00479. rumA. 1 hit.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
PS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UZK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGIIKGVSN DGLGVLGEVL VPFAYPGDVV EVISTRERFG RTIARDFKLV
60 70 80 90 100
KSSPIRVPGK CRYFGRCGGC LWQGLKYREQ LKLKEEIFKR VTGVEAEIKG
110 120 130 140 150
SPRIWFFRNI SNFIVTVNGI GFKEFGMPRT VVSVDECPVF SERTKLYIRA
160 170 180 190 200
MKRFLRETGL NPWNWKNGDV HYLQVREGKF TGEVMINVIA HIPPSGREEL
210 220 230 240 250
TEAFGFADSV YWSLKRDKRD DPKGIPTLIK GNEFIRESIE GLVYLIHPST
260 270 280 290 300
FFQTNSYALP ILLKAVESFA EGSKVLDLYS GVGTFSLYLA KKGFEVTGVE
310 320 330 340 350
VNEESVRVAK KSAEVNSLDV SFIPGRAEDA KLKGYETLIV DPPRKGLKDF
360 370 380 390 400
SKRIAKEGPE NLIYVSCNPS KFVLDYRNYL SKAYKIEDAV LIDMFPHTPH
410
VEAVVKLRRR
Length:410
Mass (Da):46,442
Last modified:May 1, 2000 - v1
Checksum:i5315A0A418E34024
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248286 Genomic DNA. Translation: CAB50056.1.
HE613800 Genomic DNA. Translation: CCE70562.1.
PIRiC75094.
RefSeqiWP_010868262.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB50056; CAB50056; PAB0760.
GeneIDi1496508.
KEGGipab:PAB0760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248286 Genomic DNA. Translation: CAB50056.1.
HE613800 Genomic DNA. Translation: CCE70562.1.
PIRiC75094.
RefSeqiWP_010868262.1. NC_000868.1.

3D structure databases

ProteinModelPortaliQ9UZK1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272844.PAB0760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB50056; CAB50056; PAB0760.
GeneIDi1496508.
KEGGipab:PAB0760.

Phylogenomic databases

eggNOGiarCOG00123. Archaea.
COG2265. LUCA.
HOGENOMiHOG000029870.
OMAiQTNSYAL.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR001566. 23S_rRNA_MeTrfase_RlmD.
IPR030390. MeTrfase_TrmA_AS.
IPR030391. MeTrfase_TrmA_CS.
IPR012340. NA-bd_OB-fold.
IPR029063. SAM-dependent_MTases.
IPR015985. TehB-like_dom.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF03848. TehB. 1 hit.
PF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00479. rumA. 1 hit.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
PS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.
  3. "Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases."
    Auxilien S., Rasmussen A., Rose S., Brochier-Armanet C., Husson C., Fourmy D., Grosjean H., Douthwaite S.
    RNA 17:45-53(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiARLMC_PYRAB
AccessioniPrimary (citable) accession number: Q9UZK1
Secondary accession number(s): G8ZKB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 2000
Last modified: November 11, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.