##gff-version 3
Q9UZ14	UniProtKB	Chain	1	625	.	.	.	ID=PRO_0000101107;Note=Threonine--tRNA ligase	
Q9UZ14	UniProtKB	Region	1	143	.	.	.	Note=Editing domain;Ontology_term=ECO:0000255,ECO:0000305,ECO:0000305,ECO:0000305,ECO:0000305;evidence=ECO:0000255|HAMAP-Rule:MF_00184,ECO:0000305|PubMed:15908961,ECO:0000305|PubMed:16902403,ECO:0000305|PubMed:21098258,ECO:0000305|PubMed:26113036;Dbxref=PMID:15908961,PMID:16902403,PMID:21098258,PMID:26113036	
Q9UZ14	UniProtKB	Region	206	505	.	.	.	Note=Catalytic;Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_00184	
Q9UZ14	UniProtKB	Binding site	298	298	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_00184	
Q9UZ14	UniProtKB	Binding site	350	350	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_00184	
Q9UZ14	UniProtKB	Binding site	474	474	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_00184	
Q9UZ14	UniProtKB	Mutagenesis	83	83	.	.	.	Note=Isolated domain has decreased to no deacylation of mischarged L-seryl-tRNA(Thr). Deacylates correctly charged glycyl-tRNA(Gly). H->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16902403,ECO:0000269|PubMed:26113036;Dbxref=PMID:16902403,PMID:26113036	
Q9UZ14	UniProtKB	Mutagenesis	120	120	.	.	.	Note=Isolated domain has nearly wild-type deacylation of mischarged L-seryl-tRNA(Thr)%2C no longer binds L-Ser or L-Cys. No activity on threonyl-tRNA(Thr). Same results%3B when associated with A-134. Y->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16902403,ECO:0000269|PubMed:26113036;Dbxref=PMID:16902403,PMID:26113036	
Q9UZ14	UniProtKB	Mutagenesis	121	121	.	.	.	Note=Isolated domain has nearly wild-type deacylation of mischarged L-seryl-tRNA(Thr). Later shown not to deacylate L-seryl-tRNA(Thr)%2C no activity on L-threonyl-tRNA(Thr). K->M;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16902403,ECO:0000269|PubMed:26113036;Dbxref=PMID:16902403,PMID:26113036	
Q9UZ14	UniProtKB	Mutagenesis	121	121	.	.	.	Note=Isolated domain does not deacylate mischarged L-seryl-tRNA(Thr). K->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16902403;Dbxref=PMID:16902403	
Q9UZ14	UniProtKB	Mutagenesis	134	134	.	.	.	Note=Isolated domain has nearly wild-type deacylation of mischarged L-seryl-tRNA(Thr). No activity on L-threonyl-tRNA(Thr). Same results%3B when associated with A-120. E->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16902403,ECO:0000269|PubMed:26113036;Dbxref=PMID:16902403,PMID:26113036	
Q9UZ14	UniProtKB	Beta strand	2	22	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Helix	28	31	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Beta strand	32	44	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Helix	47	51	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Helix	53	71	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Beta strand	75	80	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Helix	82	84	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Beta strand	86	88	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Helix	91	107	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Beta strand	111	114	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Beta strand	117	127	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Beta strand	133	138	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4RRQ	
Q9UZ14	UniProtKB	Helix	142	144	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3PD2