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Q9UZ14 (SYT_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Threonine--tRNA ligase

EC=6.1.1.3
Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS
Gene names
Name:thrS
Ordered Locus Names:PYRAB13430
ORF Names:PAB1490
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length625 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP-Rule MF_00184

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00184

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00184.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processthreonyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

threonine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 625625Threonine--tRNA ligase HAMAP-Rule MF_00184
PRO_0000101107

Regions

Region206 – 505300Catalytic HAMAP-Rule MF_00184

Sites

Metal binding2981Zinc; catalytic By similarity
Metal binding3501Zinc; catalytic By similarity
Metal binding4741Zinc; catalytic By similarity

Secondary structure

.......................... 625
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9UZ14 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: AF8E48AAE789124F

FASTA62573,372
        10         20         30         40         50         60 
MRVLLIHSDY IEYEVKDKAL KNPEPISEDM KRGRMEEVLV AFISVEKVDE KNPEEVSLKA 

        70         80         90        100        110        120 
IEEISKVAEQ VKAENVFVYP FAHLSSELAK PSVAMDILNR VYQGLKERGF NVGKAPFGYY 

       130        140        150        160        170        180 
KAFKISCKGH PLAELSRTIV PEEARVEEVP EALRKEEEEL VSYWYILTPE GELIEVDKFD 

       190        200        210        220        230        240 
FTGYENLRKF VNYEIAKNRI AEKEPPHVKL MLEHELVDYE PGSDPGNLRY YPKGRLIKSL 

       250        260        270        280        290        300 
LEQYVTEKVI EYGAMEVETP IMYDFEHPAL EKYLNRFPAR QYIVLSGDKR YFLRFAACFG 

       310        320        330        340        350        360 
QFMIKKDAII SYRNLPLRMY ELTRYSFRRE KRGELSGLRR LRAFTMPDMH TLAKDIEQAK 

       370        380        390        400        410        420 
EEFKKQFKLS MEVLEGVGLT PEDYEVAIRF TEDFWKEHKD FIVELVKLIG KPVLIEMWKQ 

       430        440        450        460        470        480 
RFFYFILKFE FNFVDNLDKA AALSTVQIDV ENAERFGITY YDENGEEKYP LILHCSPSGA 

       490        500        510        520        530        540 
IERVMYAILE KQAKLMNEGK KPMFPLWLSP IQVRVIPVSE EYLDYALYVA GKLEGAKIRV 

       550        560        570        580        590        600 
DVDDEDERLN KKIRRAEKEW IPYIVVVGAR EKENGTITVR RREDGKQYET RIEELIKEIK 

       610        620 
EKTEGFPYKP RPLPLLLSKR PKFRG 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248287 Genomic DNA. Translation: CAB50248.1.
HE613800 Genomic DNA. Translation: CCE70785.1.
PIRC75044.
RefSeqNP_127018.1. NC_000868.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y2QX-ray1.95A1-143[»]
2HKZX-ray2.10A1-143[»]
2HL0X-ray1.86A1-143[»]
2HL1X-ray2.25A/B1-147[»]
2HL2X-ray2.60A/B1-143[»]
3PD2X-ray1.86A/B1-147[»]
3PD3X-ray1.86A/B1-147[»]
3PD4X-ray2.40A/B1-147[»]
3PD5X-ray2.29A/B1-147[»]
ProteinModelPortalQ9UZ14.
SMRQ9UZ14. Positions 1-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272844.PAB1490.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB50248; CAB50248; PAB1490.
GeneID1496731.
KEGGpab:PAB1490.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0441.
HOGENOMHOG000073571.
KOK01868.
OMARQYRFRQ.

Family and domain databases

Gene3D3.40.50.800. 1 hit.
HAMAPMF_00184. Thr_tRNA_synth.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
IPR015011. Threonyl-tRNA_syn_edit_dom_arc.
[Graphical view]
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF08915. tRNA-Thr_ED. 1 hit.
[Graphical view]
PRINTSPR01047. TRNASYNTHTHR.
ProDomPD016189. tRNA-Thr_ED_arc. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF52954. SSF52954. 1 hit.
TIGRFAMsTIGR00418. thrS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9UZ14.

Entry information

Entry nameSYT_PYRAB
AccessionPrimary (citable) accession number: Q9UZ14
Secondary accession number(s): G8ZHE8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries