Threonine--tRNA ligase - Pyrococcus abyssi (strain GE5 / Orsay)

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Q9UZ14 (SYT_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	PYRAB13430
ORF Names:	PAB1490

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus ›

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP-Rule MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 625

625

Threonine--tRNA ligase HAMAP-Rule MF_00184

PRO_0000101107

Regions

Region

206 – 505

300

Catalytic HAMAP-Rule MF_00184

Sites

Metal binding

298

Zinc; catalytic By similarity

Metal binding

350

Zinc; catalytic By similarity

Metal binding

474

Zinc; catalytic By similarity

Secondary structure

625

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9UZ14 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: AF8E48AAE789124F
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FASTA

625

73,372

        10         20         30         40         50         60 
MRVLLIHSDY IEYEVKDKAL KNPEPISEDM KRGRMEEVLV AFISVEKVDE KNPEEVSLKA 

        70         80         90        100        110        120 
IEEISKVAEQ VKAENVFVYP FAHLSSELAK PSVAMDILNR VYQGLKERGF NVGKAPFGYY 

       130        140        150        160        170        180 
KAFKISCKGH PLAELSRTIV PEEARVEEVP EALRKEEEEL VSYWYILTPE GELIEVDKFD 

       190        200        210        220        230        240 
FTGYENLRKF VNYEIAKNRI AEKEPPHVKL MLEHELVDYE PGSDPGNLRY YPKGRLIKSL 

       250        260        270        280        290        300 
LEQYVTEKVI EYGAMEVETP IMYDFEHPAL EKYLNRFPAR QYIVLSGDKR YFLRFAACFG 

       310        320        330        340        350        360 
QFMIKKDAII SYRNLPLRMY ELTRYSFRRE KRGELSGLRR LRAFTMPDMH TLAKDIEQAK 

       370        380        390        400        410        420 
EEFKKQFKLS MEVLEGVGLT PEDYEVAIRF TEDFWKEHKD FIVELVKLIG KPVLIEMWKQ 

       430        440        450        460        470        480 
RFFYFILKFE FNFVDNLDKA AALSTVQIDV ENAERFGITY YDENGEEKYP LILHCSPSGA 

       490        500        510        520        530        540 
IERVMYAILE KQAKLMNEGK KPMFPLWLSP IQVRVIPVSE EYLDYALYVA GKLEGAKIRV 

       550        560        570        580        590        600 
DVDDEDERLN KKIRRAEKEW IPYIVVVGAR EKENGTITVR RREDGKQYET RIEELIKEIK 

       610        620 
EKTEGFPYKP RPLPLLLSKR PKFRG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.
[2]	"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638." Gao J., Wang J. Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: GE5 / Orsay.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ248287 Genomic DNA. Translation: CAB50248.1.
HE613800 Genomic DNA. Translation: CCE70785.1.

PIR

C75044.

RefSeq

NP_127018.1. NC_000868.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Y2Q	X-ray	1.95	A	1-143	[»]
2HKZ	X-ray	2.10	A	1-143	[»]
2HL0	X-ray	1.86	A	1-143	[»]
2HL1	X-ray	2.25	A/B	1-147	[»]
2HL2	X-ray	2.60	A/B	1-143	[»]
3PD2	X-ray	1.86	A/B	1-147	[»]
3PD3	X-ray	1.86	A/B	1-147	[»]
3PD4	X-ray	2.40	A/B	1-147	[»]
3PD5	X-ray	2.29	A/B	1-147	[»]

ProteinModelPortal

Q9UZ14.

SMR

Q9UZ14. Positions 1-147.

ModBase

Search...

Protein-protein interaction databases

STRING

272844.PAB1490.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB50248; CAB50248; PAB1490.

GeneID

1496731.

KEGG

pab:PAB1490.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0441.

HOGENOM

HOG000073571.

K01868.

OMA

MYELTRY.

ProtClustDB

PRK03991.

Family and domain databases

Gene3D

3.40.50.800. 1 hit.

HAMAP

MF_00184. Thr_tRNA_synth.

InterPro

IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
IPR015011. Threonyl-tRNA_syn_edit_dom_arc.
[Graphical view]

Pfam

PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF08915. tRNA-Thr_ED. 1 hit.
[Graphical view]

PRINTS

PR01047. TRNASYNTHTHR.

ProDom

PD016189. tRNA-Thr_ED_arc. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF52954. Anticodon_bd. 1 hit.

TIGRFAMs

TIGR00418. thrS. 1 hit.

PROSITE

PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9UZ14.

Entry information

Entry name

SYT_PYRAB

Accession

Primary (citable) accession number: Q9UZ14
Secondary accession number(s): G8ZHE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents