Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9UZ14

- SYT_PYRAB

UniProt

Q9UZ14 - SYT_PYRAB

Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi298 – 2981Zinc; catalyticBy similarity
    Metal bindingi350 – 3501Zinc; catalyticBy similarity
    Metal bindingi474 – 4741Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. threonine-tRNA ligase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. threonyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Threonine--tRNA ligase (EC:6.1.1.3)
    Alternative name(s):
    Threonyl-tRNA synthetase
    Short name:
    ThrRS
    Gene namesi
    Name:thrS
    Ordered Locus Names:PYRAB13430
    ORF Names:PAB1490
    OrganismiPyrococcus abyssi (strain GE5 / Orsay)
    Taxonomic identifieri272844 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000810: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 625625Threonine--tRNA ligasePRO_0000101107Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272844.PAB1490.

    Structurei

    Secondary structure

    1
    625
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 2221
    Helixi28 – 303
    Beta strandi31 – 4414
    Helixi47 – 515
    Helixi53 – 7119
    Beta strandi75 – 806
    Helixi82 – 843
    Beta strandi86 – 883
    Helixi91 – 10717
    Beta strandi111 – 1144
    Beta strandi117 – 12711
    Beta strandi133 – 1386
    Helixi142 – 1443

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Y2QX-ray1.95A1-143[»]
    2HKZX-ray2.10A1-143[»]
    2HL0X-ray1.86A1-143[»]
    2HL1X-ray2.25A/B1-147[»]
    2HL2X-ray2.60A/B1-143[»]
    3PD2X-ray1.86A/B1-147[»]
    3PD3X-ray1.86A/B1-147[»]
    3PD4X-ray2.40A/B1-147[»]
    3PD5X-ray2.29A/B1-147[»]
    ProteinModelPortaliQ9UZ14.
    SMRiQ9UZ14. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9UZ14.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni206 – 505300CatalyticAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0441.
    HOGENOMiHOG000073571.
    KOiK01868.
    OMAiRQYRFRQ.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    HAMAPiMF_00184. Thr_tRNA_synth.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002320. Thr-tRNA-ligase_IIa.
    IPR015011. Threonyl-tRNA_syn_edit_dom_arc.
    [Graphical view]
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF08915. tRNA-Thr_ED. 1 hit.
    [Graphical view]
    PRINTSiPR01047. TRNASYNTHTHR.
    ProDomiPD016189. tRNA-Thr_ED_arc. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF52954. SSF52954. 1 hit.
    TIGRFAMsiTIGR00418. thrS. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9UZ14-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVLLIHSDY IEYEVKDKAL KNPEPISEDM KRGRMEEVLV AFISVEKVDE    50
    KNPEEVSLKA IEEISKVAEQ VKAENVFVYP FAHLSSELAK PSVAMDILNR 100
    VYQGLKERGF NVGKAPFGYY KAFKISCKGH PLAELSRTIV PEEARVEEVP 150
    EALRKEEEEL VSYWYILTPE GELIEVDKFD FTGYENLRKF VNYEIAKNRI 200
    AEKEPPHVKL MLEHELVDYE PGSDPGNLRY YPKGRLIKSL LEQYVTEKVI 250
    EYGAMEVETP IMYDFEHPAL EKYLNRFPAR QYIVLSGDKR YFLRFAACFG 300
    QFMIKKDAII SYRNLPLRMY ELTRYSFRRE KRGELSGLRR LRAFTMPDMH 350
    TLAKDIEQAK EEFKKQFKLS MEVLEGVGLT PEDYEVAIRF TEDFWKEHKD 400
    FIVELVKLIG KPVLIEMWKQ RFFYFILKFE FNFVDNLDKA AALSTVQIDV 450
    ENAERFGITY YDENGEEKYP LILHCSPSGA IERVMYAILE KQAKLMNEGK 500
    KPMFPLWLSP IQVRVIPVSE EYLDYALYVA GKLEGAKIRV DVDDEDERLN 550
    KKIRRAEKEW IPYIVVVGAR EKENGTITVR RREDGKQYET RIEELIKEIK 600
    EKTEGFPYKP RPLPLLLSKR PKFRG 625
    Length:625
    Mass (Da):73,372
    Last modified:May 1, 2000 - v1
    Checksum:iAF8E48AAE789124F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ248287 Genomic DNA. Translation: CAB50248.1.
    HE613800 Genomic DNA. Translation: CCE70785.1.
    PIRiC75044.
    RefSeqiNP_127018.1. NC_000868.1.
    WP_010868458.1. NC_000868.1.

    Genome annotation databases

    EnsemblBacteriaiCAB50248; CAB50248; PAB1490.
    GeneIDi1496731.
    KEGGipab:PAB1490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ248287 Genomic DNA. Translation: CAB50248.1 .
    HE613800 Genomic DNA. Translation: CCE70785.1 .
    PIRi C75044.
    RefSeqi NP_127018.1. NC_000868.1.
    WP_010868458.1. NC_000868.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Y2Q X-ray 1.95 A 1-143 [» ]
    2HKZ X-ray 2.10 A 1-143 [» ]
    2HL0 X-ray 1.86 A 1-143 [» ]
    2HL1 X-ray 2.25 A/B 1-147 [» ]
    2HL2 X-ray 2.60 A/B 1-143 [» ]
    3PD2 X-ray 1.86 A/B 1-147 [» ]
    3PD3 X-ray 1.86 A/B 1-147 [» ]
    3PD4 X-ray 2.40 A/B 1-147 [» ]
    3PD5 X-ray 2.29 A/B 1-147 [» ]
    ProteinModelPortali Q9UZ14.
    SMRi Q9UZ14. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272844.PAB1490.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB50248 ; CAB50248 ; PAB1490 .
    GeneIDi 1496731.
    KEGGi pab:PAB1490.

    Phylogenomic databases

    eggNOGi COG0441.
    HOGENOMi HOG000073571.
    KOi K01868.
    OMAi RQYRFRQ.

    Miscellaneous databases

    EvolutionaryTracei Q9UZ14.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    HAMAPi MF_00184. Thr_tRNA_synth.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002320. Thr-tRNA-ligase_IIa.
    IPR015011. Threonyl-tRNA_syn_edit_dom_arc.
    [Graphical view ]
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF08915. tRNA-Thr_ED. 1 hit.
    [Graphical view ]
    PRINTSi PR01047. TRNASYNTHTHR.
    ProDomi PD016189. tRNA-Thr_ED_arc. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF52954. SSF52954. 1 hit.
    TIGRFAMsi TIGR00418. thrS. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GE5 / Orsay.
    2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
      Gao J., Wang J.
      Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: GE5 / Orsay.

    Entry informationi

    Entry nameiSYT_PYRAB
    AccessioniPrimary (citable) accession number: Q9UZ14
    Secondary accession number(s): G8ZHE8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3