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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi298 – 2981Zinc; catalyticBy similarity
Metal bindingi350 – 3501Zinc; catalyticBy similarity
Metal bindingi474 – 4741Zinc; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi6.1.1.3. 5242.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Gene namesi
Name:thrS
Ordered Locus Names:PYRAB13430
ORF Names:PAB1490
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000810 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 625625Threonine--tRNA ligasePRO_0000101107Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB1490.

Structurei

Secondary structure

1
625
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 2221Combined sources
Helixi28 – 303Combined sources
Beta strandi31 – 4414Combined sources
Helixi47 – 515Combined sources
Helixi53 – 7119Combined sources
Beta strandi75 – 806Combined sources
Helixi82 – 843Combined sources
Beta strandi86 – 883Combined sources
Helixi91 – 10717Combined sources
Beta strandi111 – 1144Combined sources
Beta strandi117 – 12711Combined sources
Beta strandi133 – 1386Combined sources
Helixi142 – 1443Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y2QX-ray1.95A1-143[»]
2HKZX-ray2.10A1-143[»]
2HL0X-ray1.86A1-143[»]
2HL1X-ray2.25A/B1-147[»]
2HL2X-ray2.60A/B1-143[»]
3PD2X-ray1.86A/B1-147[»]
3PD3X-ray1.86A/B1-147[»]
3PD4X-ray2.40A/B1-147[»]
3PD5X-ray2.29A/B1-147[»]
4S02X-ray1.95A1-143[»]
4S03X-ray2.05A1-143[»]
4S0IX-ray2.36A1-143[»]
4S0JX-ray2.10A1-143[»]
4S0KX-ray2.10A1-143[»]
4S0LX-ray2.50A1-143[»]
ProteinModelPortaliQ9UZ14.
SMRiQ9UZ14. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UZ14.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni206 – 505300CatalyticAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000073571.
KOiK01868.
OMAiLRFAACF.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
IPR015011. Threonyl-tRNA_syn_edit_dom_arc.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF08915. tRNA-Thr_ED. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
ProDomiPD016189. tRNA-Thr_ED_arc. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UZ14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLLIHSDY IEYEVKDKAL KNPEPISEDM KRGRMEEVLV AFISVEKVDE
60 70 80 90 100
KNPEEVSLKA IEEISKVAEQ VKAENVFVYP FAHLSSELAK PSVAMDILNR
110 120 130 140 150
VYQGLKERGF NVGKAPFGYY KAFKISCKGH PLAELSRTIV PEEARVEEVP
160 170 180 190 200
EALRKEEEEL VSYWYILTPE GELIEVDKFD FTGYENLRKF VNYEIAKNRI
210 220 230 240 250
AEKEPPHVKL MLEHELVDYE PGSDPGNLRY YPKGRLIKSL LEQYVTEKVI
260 270 280 290 300
EYGAMEVETP IMYDFEHPAL EKYLNRFPAR QYIVLSGDKR YFLRFAACFG
310 320 330 340 350
QFMIKKDAII SYRNLPLRMY ELTRYSFRRE KRGELSGLRR LRAFTMPDMH
360 370 380 390 400
TLAKDIEQAK EEFKKQFKLS MEVLEGVGLT PEDYEVAIRF TEDFWKEHKD
410 420 430 440 450
FIVELVKLIG KPVLIEMWKQ RFFYFILKFE FNFVDNLDKA AALSTVQIDV
460 470 480 490 500
ENAERFGITY YDENGEEKYP LILHCSPSGA IERVMYAILE KQAKLMNEGK
510 520 530 540 550
KPMFPLWLSP IQVRVIPVSE EYLDYALYVA GKLEGAKIRV DVDDEDERLN
560 570 580 590 600
KKIRRAEKEW IPYIVVVGAR EKENGTITVR RREDGKQYET RIEELIKEIK
610 620
EKTEGFPYKP RPLPLLLSKR PKFRG
Length:625
Mass (Da):73,372
Last modified:May 1, 2000 - v1
Checksum:iAF8E48AAE789124F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248287 Genomic DNA. Translation: CAB50248.1.
HE613800 Genomic DNA. Translation: CCE70785.1.
PIRiC75044.
RefSeqiNP_127018.1. NC_000868.1.
WP_010868458.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB50248; CAB50248; PAB1490.
GeneIDi1496731.
KEGGipab:PAB1490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248287 Genomic DNA. Translation: CAB50248.1.
HE613800 Genomic DNA. Translation: CCE70785.1.
PIRiC75044.
RefSeqiNP_127018.1. NC_000868.1.
WP_010868458.1. NC_000868.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y2QX-ray1.95A1-143[»]
2HKZX-ray2.10A1-143[»]
2HL0X-ray1.86A1-143[»]
2HL1X-ray2.25A/B1-147[»]
2HL2X-ray2.60A/B1-143[»]
3PD2X-ray1.86A/B1-147[»]
3PD3X-ray1.86A/B1-147[»]
3PD4X-ray2.40A/B1-147[»]
3PD5X-ray2.29A/B1-147[»]
4S02X-ray1.95A1-143[»]
4S03X-ray2.05A1-143[»]
4S0IX-ray2.36A1-143[»]
4S0JX-ray2.10A1-143[»]
4S0KX-ray2.10A1-143[»]
4S0LX-ray2.50A1-143[»]
ProteinModelPortaliQ9UZ14.
SMRiQ9UZ14. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272844.PAB1490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB50248; CAB50248; PAB1490.
GeneIDi1496731.
KEGGipab:PAB1490.

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000073571.
KOiK01868.
OMAiLRFAACF.

Enzyme and pathway databases

BRENDAi6.1.1.3. 5242.

Miscellaneous databases

EvolutionaryTraceiQ9UZ14.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
IPR015011. Threonyl-tRNA_syn_edit_dom_arc.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF08915. tRNA-Thr_ED. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
ProDomiPD016189. tRNA-Thr_ED_arc. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiSYT_PYRAB
AccessioniPrimary (citable) accession number: Q9UZ14
Secondary accession number(s): G8ZHE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: May 27, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.