Q9UZ09 (ILVC_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	PYRAB13480
ORF Names:	PAB0889

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151396
Sites
	Active site	111	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9UZ09 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D9C1D0A0CE705378	FASTA	332	37,176
	10 20 30 40 50 60 MVKVVKVYYD DEVSMDILKD KTVAVIGYGN QGEAQAKNMR DSGVHVILGL RPSGSSWKRA 70 80 90 100 110 120 EKDGFEVYTI EEAVKRADIV HILIPDLVQP KVYREHIEPY LREGQALGFS HGFNIHYKQI 130 140 150 160 170 180 VPPEYVDVIM VAPKSPGKRV REKYLEGFGV PALVAVYQDY TGNAKDLALA MAKAIGCTRA 190 200 210 220 230 240 GVIETTFKDE TESDLIGEQL VLVGGLIELI KKGFEVLVEL GYPPELAYFE ACNEAKLIMD 250 260 270 280 290 300 LIYERGFTGM LKAVSDTAKY GGLTVGPKVI DDHVKENMKK FAERVRSGEF AKEWISKADK 310 320 330 ASEVLEELMK PIEEHEIEKV GRFIRKMSGL ER

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248287 Genomic DNA. Translation: CAB50253.1.
PIR	H75044.
RefSeq	NP_127023.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9UZ09.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000003007; EBPYRP00000002938; EBPYRG00000003007.
GeneID	1496737.
GenomeReviews	Gene locus PYRAB13480 in contig AL096836_GR.
KEGG	pab:PAB0889.
NMPDR	fig|272844.1.peg.1430.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000023153.
HOGENOM	HBG286525.
OMA	MAYAKGI.
PhylomeDB	Q9UZ09.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	PABY272844:PAB0889-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_PYRAB

Accession

Primary (citable) accession number: Q9UZ09

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program