Reviewed, UniProtKB/Swiss-Prot Q9UYZ0 (VORD_PYRAB)

Names and origin

Protein names

Recommended name:
    Ketoisovalerate oxidoreductase subunit vorD
      Short name=VOR
    EC=1.2.7.7
Alternative name(s):
    2-oxoisovalerate oxidoreductase delta chain
    2-oxoisovalerate ferredoxin reductase subunit delta

Gene names

Name:	vorD
Ordered Locus Names:	PYRAB13670
ORF Names:	PAB1471

Organism

Pyrococcus abyssi [Complete proteome] [HAMAP]

Taxonomic identifier

29292 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+.
Cofactor	Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain By similarity.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity Inferred from electronic annotation. Source: EC 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	Ketoisovalerate oxidoreductase subunit vorD		PRO_0000099961
Regions
	Domain	44 – 73	30	4Fe-4S ferredoxin-type 1
	Domain	74 – 103	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	53	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	56	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	63	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	83	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	86	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	89	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	93	1	Iron-sulfur 1 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9UYZ0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F7A4FFCE33C99A84	FASTA	105	11,877
	10 20 30 40 50 60 MNTLFGKAKE EARVISPKSV DEYPEAPITL GTTLVNFTGD WRTFMPVIDE SKCVKCYICW 70 80 90 100 KFCPEPAIYI KEDGFVAIDY DYCKGCGICA NECPTKAITM VREEK

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248287 Genomic DNA. Translation: CAB50272.1.
PIR	C75047.
RefSeq	NP_127042.1.
3D structure databases
SMR	Q9UYZ0. Positions 40-102.
ModBase	Search...
Genome annotation databases
GeneID	1496756.
KEGG	pab:PAB1471.
NMPDR	fig|272844.1.peg.1449.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734156.
OMA	VDYEYCK.
Enzyme and pathway databases
BioCyc	PABY272844:PAB1471-MONOMER.
BRENDA	1.2.7.7. 262861.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR011898. PorD_KorD. [Graphical view]
TIGRFAMs	TIGR02179. PorD_KorD. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VORD_PYRAB

Accession

Primary (citable) accession number: Q9UYZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)