Q9UYX4 (RIFK_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	PYRAB13830
ORF Names:	PAB0917

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Riboflavin kinase HAMAP MF_01285		PRO_0000322098
Regions
	Nucleotide binding	97 – 102	6	CDP By similarity
	Nucleotide binding	193 – 196	4	CDP By similarity
	Region	1 – 87	87	H-T-H motif-like HAMAP MF_01285
	Region	88 – 212	125	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	124	1	Magnesium By similarity
	Metal binding	126	1	Magnesium By similarity
	Binding site	180	1	FMN By similarity
	Binding site	188	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UYX4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 4A420F3ACA03C82A	FASTA	212	23,918
	10 20 30 40 50 60 MKMKTLFLLI KLAKMGAIGK EITVTMRELS RELDVSPQTV LRWLEELKEQ GYIHKRESRK 70 80 90 100 110 120 GTLVELTEKG INLLEKLYEE ISTALYSGFI VGEVISGIGE GAYYVRQYAP LIREYLGFDP 130 140 150 160 170 180 YPGTLNVRVL FPKTIFDALC TARPIIIPGF TRNGRTFGDV RAYRVRIDGI EGAIVIPSRT 190 200 210 VHPPKIAEII APVKLRDALN LKDGDKVRIE VV

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248287 Genomic DNA. Translation: CAB50288.1.
PIR	C75049.
RefSeq	NP_127058.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9UYX4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002571; EBPYRP00000002502; EBPYRG00000002571.
GeneID	1496772.
GenomeReviews	Gene locus PYRAB13830 in contig AL096836_GR.
KEGG	pab:PAB0917.
NMPDR	fig|272844.1.peg.1465.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000023005.
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	CLSK253495.
Enzyme and pathway databases
BioCyc	PABY272844:PAB0917-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR001034. Tscrpt_reg_HTH_DeoR_N. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. PF08220. HTH_DeoR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_PYRAB

Accession

Primary (citable) accession number: Q9UYX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program